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Kim, Miryung; Gee, M.; Loh, Alex; Rachatasumrit, Napol

doi:10.1145/1882291.1882353

Cited by 110 publications

(19 citation statements)

References 11 publications

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“…Each plate contained triplicates of nontemplate control and calibrator. Relative expression was determined using GenEx software using algorithms fromVandesompele et al rna18s and ef1a were validated as reference genes using RefFinder and used for data normalization.…”

Section: Methodsmentioning

confidence: 99%

Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels

Ciani

Fontaine

Maugars

et al. 2019

Journal of Pineal Research

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The hormone melatonin connects environmental cues, such as photoperiod and temperature, with a number of physiological and behavioural processes, including seasonal reproduction, through binding to their cognate receptors. This study reports the structural, functional and physiological characterization of five high‐affinity melatonin receptors (Mtnr1aaα, Mtnr1aaβ, Mtnr1ab, Mtnr1al, Mtnr1b) in Atlantic salmon. Phylogenetic analysis clustered salmon melatonin receptors into three monophyletic groups, Mtnr1A, Mtnr1Al and Mtnr1B, but no functional representative of the Mtnr1C group. Contrary to previous studies in vertebrates, pharmacological characterization of four receptors in COS‐7, CHO and SH‐SY5Y cell lines (Mtnr1Aaα, Mtnr1Aaβ, Mtnr1Ab, Mtnr1B) showed induction of intracellular cAMP levels following 2‐iodomelatonin or melatonin exposure. No consistent response was measured after N‐acetyl‐serotonin or serotonin exposure. Melatonin receptor genes were expressed at all levels of the hypothalamo‐pituitary‐gonad axis, with three genes (mtnr1aaβ, mtnr1ab and mtnr1b) detected in the pituitary. Pituitary receptors displayed daily fluctuations in mRNA levels during spring, prior to the onset of gonadal maturation, but not in autumn, strongly implying a direct involvement of melatonin in seasonal processes regulated by the pituitary. To the best of our knowledge, this is the first report of cAMP induction mediated via melatonin receptors in a teleost species.

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Section: Methodsmentioning

confidence: 99%

Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels

Ciani

Fontaine

Maugars

et al. 2019

Journal of Pineal Research

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“…• To achieve extensibility, WitchDoctor employs a declarative specification language for describing refactoring operations, akin to Kim's language [5]. In section II, we examine related work as it informs our solutions to the five challenges above.…”

Section: ) Extensibilitymentioning

confidence: 99%

“…The specifications are designed using a declarative rulebased model, derived from Kim's body of research on detecting refactorings in software repositories [21], [5]. Each refactoring is described by one or more "specifications".…”

Section: {Covers Coveredby}mentioning

confidence: 99%

WitchDoctor: IDE support for real-time auto-completion of refactorings

Foster

Griswold

Lerner

2012

2012 34th International Conference on Software Engineering (ICSE)

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Abstract-Integrated Development Environments (IDEs) have come to perform a wide variety of tasks on behalf of the programmer, refactoring being a classic example. These operations have undeniable benefits, yet their large (and growing) number poses a cognitive scalability problem. Our main contribution is WitchDoctor -a system that can detect, on the fly, when a programmer is hand-coding a refactoring. The system can then complete the refactoring in the background and propose it to the user long before the user can complete it. This implies a number of technical challenges. The algorithm must be 1) highly efficient, 2) handle unparseable programs, 3) tolerate the variety of ways programmers may perform a given refactoring, 4) use the IDE's proven and familiar refactoring engine to perform the refactoring, even though the the refactoring has already begun, and 5) support the wide range of refactorings present in modern IDEs. Our techniques for overcoming these challenges are the technical contributions of this paper.We evaluate WitchDoctor's design and implementation by simulating over 5,000 refactoring operations across three opensource projects. The simulated user is faster and more efficient than an average human user, yet WitchDoctor can detect more than 90% of refactoring operations as they are being performed -and can complete over a third of refactorings before the simulated user does. All the while, WitchDoctor remains robust in the face of non-parseable programs and unpredictable refactoring scenarios. We also show that WitchDoctor is efficient enough to perform computation on a keystroke-by-keystroke basis, adding an average overhead of only 15 milliseconds per keystroke.

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“…The reference genes used for data normalization were rna18s and ef1a . Stability of the reference genes was tested using the online tool RefFinder (Kim et al, 2010).…”

Section: Methodsmentioning

confidence: 99%

Sexual maturation in Atlantic salmon male parr may be triggered both in early spring and late summer under standard farming conditions

Ciani

Krogh

Nourizadeh-Lillabadi

et al. 2021

Preprint

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Male Atlantic salmon (Salmo salar) display different sexual strategies, maturing either as parr during the freshwater phase (as sneaky spawners), or as post smolts following one or several years at sea. First sexual maturation (puberty) occurs at different times depending on environmental and genetic factors. To improve our knowledge on the timing (age and season) of first sexual maturation in Atlantic salmon male parr, we investigated pubertal activation in second generation farmed salmon from the Norwegian river Figgjo, reared under natural conditions of photoperiod and water temperature. Histological analysis, in combination with morphometric measurements, plasma androgen levels and pituitary gonadotropin gene expression analysis revealed that, as previously reported, some male parr initiated early sexual maturation in spring at one year of age. Interestingly, some male parr were observed to initiate sexual maturation already in autumn, six months after hatching (under-yearlings), much earlier than reported in previous studies. One-year old maturing males showed a low induction in gonadotropin levels, while under-yearling maturing males displayed a significant increase in fshb transcripts as compared to immature fish. Plasma testosterone, detectable also in immature males, increased constantly during testes development, while 11-ketotestosterone, undetectable in immature and early maturing males, increased during more advanced stages of maturation. A mild feminization of the testes (ovotestes) was detected in a subset of samples. This study brings new knowledge on the little investigated field of sexually maturing under-yearlings in Atlantic salmon. This is also the first study comparing the physiology of under-yearling vs one-year old maturing male parr, thus bringing new insights to the remarkable plasticity of Atlantic salmon puberty.

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Ref-Finder

Cited by 110 publications

References 11 publications

Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels

Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels

WitchDoctor: IDE support for real-time auto-completion of refactorings

Sexual maturation in Atlantic salmon male parr may be triggered both in early spring and late summer under standard farming conditions

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