Refeeding after caloric restriction reverses altered liver glucose release

Garcia, Renata Porto Alegre; Mariano, Isabela Ramos; Stolarz, Isabela C.; Pedrosa, Maria Montserrat Diaz

doi:10.1080/13813455.2017.1370000

Cited by 4 publications

(8 citation statements)

References 21 publications

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“…For the most part, results here match those of previous studies Vitoriano et al, 2011;Babata et al, 2014;Garcia et al, 2017): rats under caloric restriction, even after overnight fasting, showed high basal glucose output, responded markedly to the glycogenolytic action of adrenaline and did not increase glucose output in the presence of the gluconeogenic substrate alanine. This metabolic profile is similar to that found in rats in prandial or early post-prandial state -that is, subjected to perfusion soon after feeding (Babata et al, 2014).…”

Section: Discussionsupporting

confidence: 90%

“…Our research group has been reporting changes of liver glucose metabolism caused by caloric restriction since lactation Vitoriano et al, 2011;Babata et al, 2014;Garcia et al, 2017). In those studies, rats from 6-pups litters fed ad libitum after lactation had decreased glucose output and enhanced gluconeogenesis during in situ liver perfusion after overnight fasting, as expected, because fasting decreases liver glycogen stores and favors gluconeogenesis (Rui, 2014).…”

Section: Introductionmentioning

confidence: 73%

“…In the previous studies of the research group, the small (6 pups) litters were included for biometric comparison with the large ones (12 pups), but only the latter were subjected to caloric restriction and/or refeeding Vitoriano et al, 2011;Babata et al, 2014;Garcia et al, 2017). Therefore, the liver metabolic profile could be the result of some residual influence of the limited food supply during lactation and, as such, might not be present if animals from small litters were food restricted.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, rats under caloric restriction since birth (raised in 12-pups litters and food-restricted to 50% after lactation) had a completely different profile: high glucose output and almost undetected gluconeogenesis after overnight fasting Vitoriano et al, 2011), very similar to animals in post-prandial state (Babata et al, 2014). In another investigation, free feeding after caloric restriction (refeeding) decreased adrenaline-stimulated glycogenolysis after overnight fasting (Garcia et al, 2017), suggesting that the changes in glucose metabolism were not due to litter size (that is, lactational nutrition), but to the chronic feeding regimen and, as such, reversible.…”

Section: Introductionmentioning

confidence: 95%

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Differential Responses of Liver and Hypothalamus to the Nutritional Condition During Lactation and Adult Life

et al. 2020

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It was previously reported that liver glucose metabolism in rats under caloric restriction differs from that of freely-fed rats. This study hypothesized that these changes (1) were related to the expression of hypothalamic neuropeptides involved in metabolic control, and (2) were not a residual effect of litter size. To those purposes, liver glucose metabolism and hypothalamic expression of the orexigenic neuropeptides NPY (neuropeptide Y) and AgRP (agouti gene-related peptide); and of the anorexigenic neuropeptides POMC (pro-opiomelanocortin) and CART (cocaineand amphetamine-related transcripts) were investigated. Male Wistar rats from two different litter sizes (G6 and G12, with 6 or 12 pups, respectively) were subjected to free feeding (GL, ad libitum), 50% caloric restriction (GR) or caloric restriction+ad libitum refeeding (GRL) until the age of 90 days. Biometric values were lower in GR than in GL, while in GRL they were totally or partially recovered. Blood glucose variation during the pyruvate tolerance test (PTT) was small in GR. During in situ liver perfusion, total, basal, and adrenaline-stimulated liver glucose outputs were high in GR, but additional glucose output in the presence of alanine was negligible. Refeeding (GRL) yielded values close to those of GL. Litter size did not consistently influence any of these variables. The expression of transcripts of the hypothalamic neuropeptides was responsive to feeding regimen, litter size and/or their interaction and differed from G6 to G12, while the metabolic changes of the liver were qualitatively equal in both GR. Therefore, the changes in glucose metabolism in the liver of rats under caloric restriction were not determined by either litter size or hypothalamic neuropeptide expression and were linked only to the prevailing feeding regimen of the adult animal.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

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Differential Responses of Liver and Hypothalamus to the Nutritional Condition During Lactation and Adult Life

et al. 2020

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“…Glucose output during basal perfusion of the liver represents the release of glucose previously formed and/or stored in the hepatocytes. As the perfusion fluid is devoid of glucose, chemical gradient favors glucose exportation from the liver cells to the fluid [20,21]. After 6 hours at postprandial state, the endogenous stores of glycogen of the mice were, at least, decreased [21], so that a gluconeogenic contribution for glucose output should be considerable.…”

Section: Discussionmentioning

confidence: 99%

Resistance High-Intensity Interval Training (HIIT) Improves Acute Gluconeogenesis from Lactate in Mice

Müller¹

2019

AJSS

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High-intensity interval training (HIIT) markedly activates muscle anaerobic glycolysis and increases blood lactate.As the liver is a major organ for lactate clearance from the bloodstream, it might improve gluconeogenesis from lactate (NEO-lac) after a period of resistance HIIT. NEO-lac was evaluated by in situ liver perfusion in mice subjected to a resistance HIIT for 4 (T4) or 8 (T8) weeks, or not trained (T0). Perfusion was carried out immediately after an incremental exercise session to test the acute NEO-lac. Muscle strength (expressed as relative maximum load) and blood lactate were higher in T4 than in T0, but NEO-lac did not differ, possibly because of energy discharge of the liver and substrate overload. After 8 weeks of HIIT (T8), both muscle strength and liver NEO-lac increased, but blood lactate did not. The resistance HIIT for 8 weeks modulated liver gluconeogenic efficiency and capacity, which are important mechanisms for the improved clearance of blood lactate.

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Mitochondrial ATP concentration decreases immediately after glucose administration to glucose‐deprived hepatocytes

Tsuno,

Harada,

Horikoshi

et al. 2023

FEBS Open Bio

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Hepatocytes can switch their metabolic processes in response to nutrient availability. However, the dynamics of metabolites (such as lactate, pyruvate, and ATP) in hepatocytes during the metabolic switch remain unknown. In this study, we visualized metabolite dynamics in primary cultured hepatocytes during recovery from glucose‐deprivation. We observed a decrease in the mitochondrial ATP concentration when glucose was administered to hepatocytes under glucose‐deprivation conditions. In contrast, there was slight change in the cytoplasmic ATP concentration. A decrease in mitochondrial ATP concentration was associated with increased protein synthesis rather than glycogen synthesis, activation of urea cycle, and production of reactive oxygen species. These results suggest that mitochondrial ATP is important in switching metabolic processes in the hepatocytes.

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Refeeding after caloric restriction reverses altered liver glucose release

Abstract: Enhanced LGR under caloric restriction is not programmed by early feeding; instead, it is determined by the current nutritional conditions.

Cited by 4 publications

References 21 publications

Differential Responses of Liver and Hypothalamus to the Nutritional Condition During Lactation and Adult Life

Differential Responses of Liver and Hypothalamus to the Nutritional Condition During Lactation and Adult Life

Resistance High-Intensity Interval Training (HIIT) Improves Acute Gluconeogenesis from Lactate in Mice

Mitochondrial ATP concentration decreases immediately after glucose administration to glucose‐deprived hepatocytes

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