“…This could be due to the sample size or due to the difficulty of estimating cervical volume. Since one of the methodological difficulties when estimating cervical volume and vascularity using 3D ultrasound is defining landmarks when drawing the contours of the cervix [53,[56][57][58][59][64][65][66].The delineation between the cervix and the lower uterine segment is particularly difficult, especially early in pregnancy and at mid-gestation when the lower uterine segment is thick and the cervix often curved. It may also be difficult to clearly separate the cervix from the surrounding vaginal tissue [53, 56-59, 64, 66].…”