Reference Echovirus 7 and 19 Genomes from Nigeria

Abstract

We describe the genomes of two echovirus isolates from Nigeria as reference enterovirus species B genomes for the region. These echovirus 7 and 19 genomes have 7,411 nucleotides (nt) and 7,426 nt and were recovered from sewage-contaminated water (in 2010) and an acute flaccid paralysis case (in 2014), respectively.

Genomic Characterization of a Coxsackievirus A20 Strain Recovered from a Child with Acute Flaccid Paralysis in Nigeria

“…The genome of the isolate was amplified in overlapping fragments (2 to 3 kb each) as previously described (2). The amplicons generated were pooled and shipped to a commercial facility (MR, TX, USA), where library preparation and NextGen sequencing was done.…”

Genome Sequences of Two Dual-Serotype-Specific Echovirus 20 Strains from Nigeria

“…Both strains were isolated on a human rhabdomyosarcoma cell line (1) and subjected to a neutralization assay (1) and molecular identification (3). Both isolates were further subjected to RNA extraction, cDNA synthesis, and full-genome amplification by sequence-independent, single-primer amplification (SISPA) (7, 8) for isolate E20_2010 or to reverse transcription-PCR (RT-PCR) of overlapping fragments for isolate E20_2014 (9), followed by Illumina sequencing.…”

Genomic Characterization of an Echovirus 7 Isolate of Southeast Asian Ancestry Recovered from a Child in Nigeria with Acute Flaccid Paralysis

“…Subsequently, genomic RNA was extracted using a total RNA extraction kit (Jena Bioscience, Jena, Germany) and converted to cDNA using the Script cDNA synthesis kit (Jena Bioscience) in accordance with the manufacturer’s instructions. Amplification of the genome was done in overlapping fragments as previously described (4); fragments were pooled and shipped to a commercial sequencing facility (MR DNA, TX, USA), where library preparation (using the Nextera DNA sample preparation kit [Illumina]) and paired-end Illumina sequencing (300 cycles using the HiSeq system) were done.…”