Reference gene selection for quantitative real-time PCR in Solanum lycopersicum L. inoculated with the mycorrhizal fungus Rhizophagus irregularis

Validation of Suitable Reference Genes for RT-qPCR Data in Achyranthes bidentata Blume under Different Experimental Conditions

“…EF1-α was ranked first in both the drought and salt groups. The stability of EF1-α in A. bidentata was consistent with previous findings in Solanumly copersicum L. (Fuentes et al, 2016); however, EF1-α is unsuitable for normalization in Salicornia europaea under drought stress (Xiao et al, 2014). UBC was stable in organ group and different in development stages in root group in A. bidentata .…”

“…Therefore, it is need to select appropriate reference genes that has a consistent level of expression under specific experimental conditions. At present, several studies on the evaluation and validation of reference genes have been carried out for many plant species, such as Arabidopsis thaliana (Czechowski et al, 2005; Remans et al, 2008), potato (Nicot et al, 2005), coffee (Barsalobres-Cavallari et al, 2009; Cruz et al, 2009; de Carvalho et al, 2013), rice (Kim et al, 2003; Jain et al, 2006), tomato (Expósito-Rodríguez et al, 2008; Mascia et al, 2010), wheat (Paolacci et al, 2009), pearl millet (Shivhare and Lata, 2016), barley (Burton et al, 2004), Brassica napus (Wang et al, 2014), radish (Xu et al, 2012), apple (Perini et al, 2014), rose (Meng et al, 2013), soybean (Jian et al, 2008; Hu et al, 2009), sugarcane (Iskandar et al, 2004), peanut (Jiang et al, 2011), tobacco (Schmidt and Delaney, 2010), banana (Chen et al, 2011), Buchloe dactyloides (Li W. et al, 2015), Salicornia europaea (Xiao et al, 2014), flixweed (Xu et al, 2016), kiwifruit (Ferradás et al, 2016), pear (Xu et al, 2015), and Solanum lycopersicum L. (Fuentes et al, 2016). However, systematic evaluation of the selection of suitable reference genes for RT-qPCR data normalization in Achyranthes bidentata under various experiments has not been reported.…”

Evaluation of duplicated reference genes for quantitative real-time PCR analysis in genome unknown hexaploid oat (Avena sativa L.)

“…Single-copy genes are usually used as RGs, although they only account for a small proportion in the genomes [30,31] of polyploids. In fact, it is worth noting that most researches on RG selection in polyploids neither display nor discuss the copy number of candidate RGs [32][33][34][35][36]. However, with the widespread of omics technology, some of these "single-copy"…”

Selection of reference genes for tissue/organ samples on day 3 fifth‐instar larvae in silkworm, Bombyx mori

“…To obtain accurate expression data, it is essential to validate the stability of the internal gene(s) before designing quantitative gene expression studies. In the past years, many reports have identified endogenous reference genes in different species (An, Hou, & Liu, ; Che Omar, Bentley, Morieri, Preston, & Gurr, ; Chen et al., ; Delporte, Legrand, Hilbert, & Gagneul, ; Etich, Bergmeier, Pitzler, & Brachvogel, ; Etschmann, Wilcken, Stoevesand, von der Schulenburg, & Sterner‐Kock, ; Exposito‐Rodriguez, Borges, Borges‐Perez, & Perez, ; Fuentes et al., ; Hamalainen et al., ; Huis, Hawkins, & Neutelings, ; Ishii et al., ; Jian et al., ; Koenig et al., ; Ma, Xu, Zhao, Xia, & Wang, ; Niu et al., ; Robinson, Sutherland, & Sutherland, ; Stern‐Straeter, Bonaterra, Hormann, Kinscherf, & Goessler, ; Tan, Zhou, & Pang, ; Yan & Liou, ).…”