Reference genes for accurate evaluation of expression levels in Trichophyton interdigitale grown under different carbon sources, pH levels and phosphate levels

Ciesielska, Anita; Oleksak, Beata; Stączek, Paweł

doi:10.1038/s41598-019-42065-5

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…The transcription profiles of the PKS gene, related to the biosynthesis TeA in A. alternata TEF-1A, were analysed by qRT-PCR. EF1-α gene was used as a reference gene (Ciesielska et al, 2019). Primer 3 was used to amplify the PKS gene, and the reference A. alternata genome can be found at http://alter naria.vbi.vt.edu.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Evaluating the potential of Pediococcus pentosaceus as a biocontrol agent against tenuazonic acid–producing Alternaria alternata on livestock feeds

Oliveira

Mendonça

Veríssimo

et al. 2022

Journal of Applied Microbiology

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Aims: This study aims to demonstrate the potential of the lactic acid bacteria (LAB)Pediococcus pentosaceus LBM18 against the mycotoxin-producing Alternaria alternata TEF-1A and highlight its application as an effective grain silage inoculant to control mycotoxin contamination. Methods and Results:The antifungal properties of Ped. pentosaceus lyophilized (PPL) were assessed by evaluating its effect on A. alternata TEF-1A grown in a corn silage-based medium, which included morphological changes by Scanning Electron Microscopy (SEM) observations, growth rate, conidia production assays, and inhibition of Tenuazonic acid (TeA) production by high-performance liquid chromatography (HPLC-MS/MS) analyses. Furthermore, TeA biosynthesis was monitored for changes at the molecular level by PKS gene expression. The growth and sporulation processes of A. alternata TEF-1A were affected by Ped. pentosaceus LBM18 in a concentration-dependent manner. Moreover, a significant inhibition of TeA production (74.3%) and the transcription level of the PKS gene (42.9%) was observed. Conclusions: Ped. pentosaceus is one of the promising LAB to be applied as an inoculant for corn silage preservation, aiming to inhibit mycotoxigenic fungi growth and their mycotoxin production.Significance and impact of the study: Ped. pentosaceus could be used as an inoculant to reduce fungal and mycotoxins contamination in grain silage production.

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Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Evaluating the potential of Pediococcus pentosaceus as a biocontrol agent against tenuazonic acid–producing Alternaria alternata on livestock feeds

Oliveira

Mendonça

Veríssimo

et al. 2022

Journal of Applied Microbiology

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“…These organisms have the capacity to breakdown keratin, such as feathers, hairs, nails, horns, and claws 14 – 16 . Dermatophytes have been divided into three ecological groups: geophilic and zoophilic (i.e., Trichophyton verrucosum , Microsporum canis ), which can spread the infection to humans, and anthropophilic dermatophytes such as T. rubrum and T. interdigitale, causing dermatophytosis in humans 17 – 19 . The Chrysosporium group comprises over 60 species (i.e., A. keratinophilus , A. fulvescens , Ch.…”

Section: Introductionmentioning

confidence: 99%

Dispersal of Aphanoascus keratinophilus by the rook Corvus frugilegus during breeding in East Poland

Kitowski

Korniłłowicz-Kowalska

Bohacz

et al. 2022

Sci Rep

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The process of dispersal of the potentially disease-causing, geophilic and keratinolytic fungal strain Aphanoascus keratinophilus (the perfect, sexual stage of Chrysosporium keratinophilum) by the rook Corvus frugilegus was studied. The source of A. keratinophilus strains was pellets of the rook, thus far not considered a carrier of this particular opportunistic pathogen. Pellets collected from breeding colonies of rooks were analysed in terms of the occurrence of keratinolytic fungi with the application of the native keratin bait method. Among the 83 rook pellets analysed, 24 (29%) were infected by keratinophilic fungi. Pure cultures of the fungi were identified to species based on traditional morphological features. Traditional mycological identification was verified by the PCR–RFLP molecular identification method as well as DNA sequencing. The obtained results showed the presence of 90 Aphanoascus keratinophilus strains, 6 Chrysosporium tropicum strains, and 3 Chrysosporium pannicola strains. The PCR melting profile (PCR-MP) method was used to identify intraspecies variations of the 90 analysed A. keratinophilus strains. The dispersal of genotypes and possible pathways of A. keratinophilus dispersal and infection via rook pellets were analysed.

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Metabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation

Ciesielska

Kawa

Kanarek

et al. 2021

Sci Rep

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Keratin is important and needed for the growth of dermatophytes in the host tissue. In turn, the ability to invade keratinised tissues is defined as a pivotal virulence attribute of this group of medically important fungi. The host–dermatophyte interaction is accompanied by an adaptation of fungal metabolism that allows them to adhere to the host tissue as well as utilize the available nutrients necessary for their survival and growth. Dermatophyte infections pose a significant epidemiological and clinical problem. Trichophyton rubrum is the most common anthropophilic dermatophyte worldwide and its typical infection areas include skin of hands or feet and nail plate. In turn, Microsporum canis is a zoophilic pathogen, and mostly well known for ringworm in pets, it is also known to infect humans. The aim of the study was to compare the intracellular metabolite content in the T. rubrum and M. canis during keratin degradation using liquid chromatography system coupled with tandem mass spectrometer (LC-MS/MS). The metabolite “fingerprints” revealed compounds associated with amino acids metabolism, carbohydrate metabolism related to the glycolysis and the tricarboxylic acid cycle (TCA), as well as nucleotide and energy metabolism. The metabolites such as kynurenic acid, l-alanine and cysteine in case of T. rubrum as well as cysteine and riboflavin in case of M. canis were detected only during keratin degradation what may suggest that these compounds may play a key role in the interactions of T. rubrum and M. canis with the host tissue. The metabolomic results were completed by qPCR gene expression assay. Our findings suggest that metabolomic analysis of T. rubrum and M. canis growing in culture media that mimic the dermatophyte infection could allow the understanding of processes involved in the pathogenesis of dermatophytes.

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Reference genes for accurate evaluation of expression levels in Trichophyton interdigitale grown under different carbon sources, pH levels and phosphate levels

Cited by 4 publications

References 42 publications

Evaluating the potential of Pediococcus pentosaceus as a biocontrol agent against tenuazonic acid–producing Alternaria alternata on livestock feeds

Evaluating the potential of Pediococcus pentosaceus as a biocontrol agent against tenuazonic acid–producing Alternaria alternata on livestock feeds

Dispersal of Aphanoascus keratinophilus by the rook Corvus frugilegus during breeding in East Poland

Metabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation

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