Reference interval for immature platelet fraction on Sysmex XN haematology analyser in adult population

Abstract

IntroductionThe Sysmex XN-series haematology analyser has newly adopted a fluorescent channel to measure immature platelet fraction (IPF). To promote the clinical utility of this promising parameter, establishing a reliable reference interval is mandatory. According to previous studies, IPF values may be affected by the employed analyser and the ethnic background of the individual, but no differences seem to be found between individuals’ genders. Therefore, this study aimed to define the reference interval for IPF in a Spanish population following Clinical and Laboratory Standard Institute (CLSI) guidelines.Materials and methodsA total of 153 healthy Caucasian adults from Spain met the inclusion criteria. IPF measurement was performed by means of a Sysmex XN-2000 haematology analyser. A non-parametric percentile method was used to calculate the reference intervals in accordance with CLSI guidelines.ResultsThe obtained reference interval for IPF on the Sysmex XN-2000 was 1.6–9.6% (90% confidence intervals (CIs) were 1.5–1.8 and 9.3–11.5, respectively). No significant gender difference in IPF reference intervals was observed (P = 0.101).ConclusionsThis study provides, for the first time, a reference interval for IPF using a Sysmex XN-2000 in a Spanish population, ranging from 1.6 to 9.6%. These data are needed to evaluate platelet production in several conditions such as thrombocytopenia, inflammatory states and cardiovascular diseases, as well as for future research.

Evaluation of the delta of immature platelet fraction as a predictive biomarker of inflammatory response after cardiac surgery

“…Additionally, the %IPF mean value obtained in both groups was in accordance to a reference interval we reported previously (1.6%–9.6%) 18. In contrast, significant differences were found between ΔIPF calculated in the noIR and IR groups.…”

Establishment of the reference intervals of whole blood neutrophil phagocytosis by flow cytometry

“…The normal distribution was expressed as mean ±standard deviation ( x ± s ), x ± 1.96 s was used as the reference interval, and t test was used for comparison. Those that do not conform to the normal distribution were represented by the median (quartile) [M (P25‐P75)], 2.5–97.5 percentile nonparametric range was used as the reference interval, 12 and the comparison between the two groups was performed by the Mann‐Whitney U test. The enumeration data were compared with the chi‐square with Fisher's exact two‐tailed p ‐value.…”

Platelet Counting: Ugly Traps and Good Advice. Proposals from the French-Speaking Cellular Hematology Group (GFHC)

“…The fluorescent dye is CD4K530 for Abbott analyzers and oxazine-based for Sysmex analyzers. Despite the poor standardization and a lack of correlation between the young platelet fraction measurements among the different instruments (absence of reference method and the existence of different reference ranges for different instruments), these parameters are good indicators of thrombopoietic activity [20][21][22]. IPF absolute count has been proven as an indicator of platelet recovery after chemotherapy in pediatrics [23] and, thus, has been proposed as a tool to categorize neonatal thrombocytopenia [24] or to predict peripheral immune thrombocytopenia [25,26].…”