Reference intervals established using indirect method for serum ferritin assayed on Abbott Architect i2000_SR analyzer in Chinese adults

Abstract

Background Serum ferritin (SF) test has been widely used in clinical practice. However, its reference intervals (RIs) vary depending on the analytical method and ethnic origin. This study was to establish the RIs using indirect method for SF in Chinese adults. Methods SF was assayed on Abbott i2000SR analyzer. The SF test results of all health examinees (8913 males aged 18‐93 years and 5397 females aged 18‐90 years) between December 2010 and April 2019 were obtained from our laboratory information system. After Box‐Cox transformation of raw data and exclusion of outliers, parametric and non‐parametric approaches were used to calculate 95% RIs. The correlation between SF levels and ages, and the differences in SF levels between subgroups were also analyzed. Results SF levels in females were significantly different from those in males (Z = 88.96, Z* = 23.17; Z > Z*) and showed a weak positive correlation with age (r = .466, P < .0001). The RIs based on parametric approach in males were 66.12‐561.58 µg/L, whereas in all females were 3.59‐269.59 µg/L, females aged <50 years 3.26‐148.02 µg/L and those aged ≥50 years 17.28‐303.27 µg/L. The RIs based on non‐parametric approach in males were 65.00‐571.37 µg/L whereas in all females were 4.00‐254.00 µg/L, females aged <50 years 4.00‐152.00 µg/L and those aged ≥50 years 16.00‐304.05 µg/L. Conclusions Our indirect RIs for SF were markedly different from the manufacturer's recommended RIs and might be more suitable for Chinese adults, which would be helpful in interpreting laboratory data and clinical decision‐making.

Gender-specific association between serum ferritin and neurodevelopment in infants aged 6 to 12 months

“…During our study period, to minimize the impact of potential batch effect on laboratory measurements, the ferritin and hemoglobin laboratory tests were performed according to the consistent platform and standard operating procedures. Serum ferritin was measured by a chemiluminescence assay using an Abbott i2000SR analyzer 12 , and hemoglobin was measured using an automated hematology analyzer (Siemens Advia 2120i) as described elsewhere 13 . Based on the World Health Organization criteria for using ferritin concentrations to assess iron status in infants and young children aged 0–23 months 14 , iron deficiency was defined as a serum ferritin level < 12 ng/mL and iron deficiency anemia (IDA) as a serum ferritin level < 12 ng/mL and hemoglobin < 110 g/L.…”

B12 deficiency‐related glossitis is highly associated with high gastrin‐17 and low pepsinogen I

“…Serum ferritin was measured by chemiluminescence microparticle immunoassay using ARCHITECT Ferritin Reagent Kit (Abbott Ireland Diagnostics Division, Longford, Ireland) with normal levels of 4.63–204 ng/mL in women and 21.8–274.66 ng/mL in men. However, Wang et al 14 proposed reference ranges suitable for the Chinese population, ranging from 66.12 to 561.58 μg/L for males, 3.26 to 148.02 μg/L for females <50 years, and 17.28 to 303.27 μg/L for females ≥50 years. Serum G17, PGI, and PGII were determined by enzyme‐linked immunosorbent assay (ELISA) using commercial kits (Biohit, Helsinki, Finland); the normal levels were 1–15 pmol/L for G17, 70–165 ng/mL for PGI, and 3–15 ng/mL for PGII, respectively; the normal range of I/II was 7–20.…”

The indirect method in the establishment of reference intervals for complement 3 and complement 4: A retrospective study

“…The Tukey method was used to remove outliers. 12 Briefly, Q1 (1st quartile) and Q3 (3rd quartile) were calculated, which involved the 25th (P25) and 75th percentiles (P75), respectively, followed by calculation of interquartile ranges (IQR: Q3 minus Q1). Thereafter, the lower and upper limits were calculated according to the formulas, P25–1.5 × IQR and P75 + 1.5 × IQR, respectively.…”

“…According to the IFCC's and the Clinical and Laboratory Standards Institute's (CLSI) recommendations, the so-called ‘direct method’ is preferred for establishing RIs, in which it is necessary to select healthy individuals' data for analysis. 12 , 13 Nevertheless, one of the difficulties in this method is that many measures are required to select healthy subjects. In addition, when establishing RIs for paediatric and geriatric groups, or those involving other uncommon sample types (e.g.…”