Reference intervals for rotational thromboelastometry measurements using the ROTEM® delta device in dogs

Pereira, Juscélia Cristina; Rohn, Karl; Mischke, R.

doi:10.1016/j.rvsc.2020.01.019

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…Parameter wise, we identified considerably higher variability and lower correlation for CT, CFT and ML compared to alpha angle, MCF, MCE and G. This is consistent with the findings of previous studies [ 7 , 29 ] but in contrast to other studies that report low variability <15% or good correlation in dogs [ 8 ] and cats [ 11 , 32 ]. The early phase of clot initiation reflected by CT is known to be mainly dependent on the activator and most sensitive to small differences regarding sample handling and choice of activator, which would explain the higher intrarater variability of CT and CFT.…”

Section: Discussionsupporting

confidence: 91%

“…In veterinary medicine, TEG/ROTEM have been validated for use in horses [ 6 , 7 ], dogs [ 8 , 9 ] and cats [ 10 , 11 ]. Although, theoretically, there are many advantages of viscoelastic tests compared to traditional coagulation testing in plasma, it is important to note that multiple preanalytical and analytical factors can influence test results [ 4 , 12 , 13 ], including sample collection technique (size of chosen vessel, amount of vessel occlusion, venipuncture technique, use of vacutainers, use of syringes), sample handling (storage time before initiation of the test, storage temperature), and the analysis itself (variability of ROTEM devices, operator variability).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Jugular vs. Saphenous Blood Samples, Intrarater and In-Between Device Reliability of Clinically Used ROTEM S Parameters in Dogs

Vuille-dit-Bille

Weingand

Schefer

et al. 2022

Animals

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Rotational Thromboelastometry (ROTEM) allows for the global assessment of hemostasis in whole blood samples. Preanalytical and analytical factors may influence test results, and data about the reliability and reproducibility of lyophilized ROTEM tests are scarce. Therefore, the objective of this study was to evaluate the influence of blood collection site on ROTEM S parameters and to assess intrarater and in-between device variability. A total of thirty, healthy, staff-owned dogs were included. Blood collection and ROTEM analysis were performed by trained staff according to a standardized protocol. Extrinsically activated (tissue factor; Ex-TEM S), with the addition of cytochalasin for platelet inhibition (Fib-TEM S), and intrinsically activated (In-TEM) analyses were performed. Analysis of our data showed significant variability for various Ex-TEM S and Fib-TEM S parameters from different collection sites and intrarater and in-between device measurements. We conclude that serial monitoring with ROTEM should be performed on the same device, with blood always taken from the same collection site using a standardized blood sampling technique. While In-TEM S, apart from maximum lysis, showed very stable and reliable results, we suggest interpreting especially clotting and clot formation parameters from Ex-TEM S and Fib-TEM S tests with caution and using duplicate measurements to detect outliers and to prevent initiation of incorrect therapies.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Comparison of Jugular vs. Saphenous Blood Samples, Intrarater and In-Between Device Reliability of Clinically Used ROTEM S Parameters in Dogs

Vuille-dit-Bille

Weingand

Schefer

et al. 2022

Animals

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“…35,38 Neuter status in female dogs has been shown to significantly influence viscoelastic analysis using the ROTEM delta analyzer. 34 However, given the small number of female dogs (n = 5; 2 spayed females) in our study, statistical analyses by sex or neuter status were not feasible.…”

Section: Discussionmentioning

confidence: 87%

Comparison of assessment of coagulation in healthy dogs by the TEG 6s and TEG 5000 viscoelastic analyzers

et al. 2022

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The TEG 6s (Haemonetics) point-of-care viscoelastic analyzer is portable, compact, simple to use, and has the potential for rapid viscoelastic analysis that can guide the treatment of veterinary patients at the site of care. Although approved for use in people, the TEG 6s has yet to be evaluated for hemostatic analysis in veterinary medicine. Citrated whole blood (CWB) was collected from 27 healthy dogs. An aliquot of CWB from each dog was diluted by 33% with an isotonic crystalloid, representing an in vitro model of hemodilution. Unaltered and diluted CWB samples were analyzed using 2 TEG 6s and 6 TEG 5000 (Haemonetics) analyzers. The 6 TEG 5000 analyzers ran duplicate analyses of either unaltered or diluted samples using 1 of 3 reagents (Haemonetics): Kaolin TEG, RapidTEG, or TEG Functional Fibrinogen. Duplicate TEG 5000 analyses were averaged and compared with a single TEG 6s analysis. Lin concordance correlation coefficient and Bland–Altman plots were used to evaluate agreement of reaction time, kinetic time, alpha angle, maximum amplitude (MA), and G value (G) for samples activated with Kaolin TEG, and agreement of MA for samples activated with RapidTEG between the 2 machines. Overall, agreement between the TEG 6s and TEG 5000 analyzers was poor. Viscoelastic measurements by the TEG 6s and TEG 5000 in healthy dogs were not all interchangeable. Agreement was satisfactory only for MA and G measurements of diluted blood samples activated with Kaolin TEG, and MA measurements for both unaltered and diluted blood samples activated with RapidTEG.

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Thrombin Generation Assay and Hemostatic Profile for Elucidating Hypercoagulability in Endogenous Canine Hyperadrenocorticism

Vargas-Mora

Romão

Santos

et al. 2021

Topics in Companion Animal Medicine

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Reference intervals for rotational thromboelastometry measurements using the ROTEM® delta device in dogs

Cited by 5 publications

References 39 publications

Comparison of Jugular vs. Saphenous Blood Samples, Intrarater and In-Between Device Reliability of Clinically Used ROTEM S Parameters in Dogs

Comparison of Jugular vs. Saphenous Blood Samples, Intrarater and In-Between Device Reliability of Clinically Used ROTEM S Parameters in Dogs

Comparison of assessment of coagulation in healthy dogs by the TEG 6s and TEG 5000 viscoelastic analyzers

Thrombin Generation Assay and Hemostatic Profile for Elucidating Hypercoagulability in Endogenous Canine Hyperadrenocorticism

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