1998
DOI: 10.1159/000019080
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Reference Typing Report for Complement Factor B

Abstract: Factor B (BF) reference typing was carried out on the occasion of the VIIth Complement Genetics Workshop in Mainz, May 1998. Two different sets of samples were analysed by agarose electrophoresis and/or isoelectric focusing at the protein level, and by PCR-RFLP analysis at the DNA level. These results confirmed the reliability of the standard agarose electrophoresis technique for the identification of the major BF alleles as well as for the identification of cathodic and anodic variants. However, the exact alp… Show more

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Cited by 7 publications
(3 citation statements)
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“…To accommodate all sequence data and to make it available to the public, a mutation database may be created on the internet. Meanwhile, convenient DNA typing techniques for C4, C2 and Bf would be developed and applied in parallel with HLA typing (for C4 and Bf see reference typing reports in this issue [103,104]). This information will prove valuable for HLA haplotype matching, for forensic purposes and for anthropologic studies.…”
Section: Discussionmentioning
confidence: 99%
“…To accommodate all sequence data and to make it available to the public, a mutation database may be created on the internet. Meanwhile, convenient DNA typing techniques for C4, C2 and Bf would be developed and applied in parallel with HLA typing (for C4 and Bf see reference typing reports in this issue [103,104]). This information will prove valuable for HLA haplotype matching, for forensic purposes and for anthropologic studies.…”
Section: Discussionmentioning
confidence: 99%
“…Factor B allotyping by agarose gel electrophoresis has identified 3 common phenotypes (F, FS, and S) and ~15 rare variants. However, using isoelectric focusing or PCR-RFLP of codon 7 two subtypes (FA and FB) can be identified (39). The frequencies of BF alleles is highly variable; for instance, BF*S is very common in Europeans while BF*F is found in African derived populations (13).…”
Section: Factor B (Bf)mentioning
confidence: 99%
“…A number of technologies including amplified fragment length polymorphism (AFLP) (17, 19), sequencing (16) and isoelectric focusing of plasma proteins (19) are available to resolve BF variants. In this study, we have designed sequence‐specific primers (SSP) corresponding to the three BF alleles and used them in a single‐step polymerase chain reaction using SSP (PCR‐SSP) assay for BF allotyping.…”
Section: Introductionmentioning
confidence: 99%