Reference Values for Neonates and Children for the UF-100 Urine Flow Cytometer

Lun, Andreas; Ziebig, Reinhard; Hammer, Hannes; Otting, U; Filler, Guido; Sinha, Pranav

doi:10.1093/clinchem/45.10.1879

Cited by 16 publications

(2 citation statements)

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“…A leucocytes count ≥35cells/μl corresponds to 10 leucocytes per high power field using microscopic examination of urine after centrifugation, the recommended limit value to document urethritis according to US guidelines. [ 9 , 15 ]…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Mycoplasma genitalium in men with urethritis in a large public hospital in Brussels, Belgium: An observational, cross-sectional study

Libois

Hallin²,

Crucitti

et al. 2018

PLoS ONE

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BackgroundMycoplasma genitalium (MG) is a cause of urethritis. While resistance to azithromycin is increasing, routine detection of MG is not performed in Belgium, where its prevalence is unknown. The aim of this study is to determine prevalence of MG in men with urethritis.Method and findingsAn “in-house” amplification assay detecting MG was performed on urine of men with complaints of urethritis who consulted the emergency unit or the Sexually Transmitted Infection clinic of our public hospital in Brussels. Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) were tested on the same sample. A total of 187 men were tested. Prevalence of MG was 9% (95% Confidence Interval: 5 to 13.2%). CT was detected in 20%, NG in 22% and 56% of samples were negative for these three pathogens. Neither age, ethnic origin, sexual orientation nor HIV infection were associated with MG urethritis.ConclusionM. genitalium was identified in 9% of men with complaints of urethritis indicating that amplification assay detecting MG should be implemented in routine testing for those patients.

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Section: Methodsmentioning

confidence: 99%

Prevalence of Mycoplasma genitalium in men with urethritis in a large public hospital in Brussels, Belgium: An observational, cross-sectional study

Libois

Hallin²,

Crucitti

et al. 2018

PLoS ONE

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“…Automated urinalysis flow cytometer such as Sysmex UF series has been adopted in clinical laboratory for almost two decades . When reporting the cell counts, the UF‐1000i system provides two different kinds of units, which are “cells per μL (non‐centrifugal urine)” and “cells per HPF (by calculated automatically).” The value of UF series is obviously higher than those obtained with microscopic examination, which brings confusion to clinicians when they are not used to the units of UF series …”

Section: Introductionmentioning

confidence: 98%

The standardization of the report for urine cell counting—A converting factor for Sysmex UF‐1000i

Wang

Zhao

et al. 2019

Clinical Laboratory Analysis

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BackgroundMulticenter laboratory may apply both automated flow cytometer and microscopy for urinalysis. Automated flow cytometer such as Sysmex UF‐1000i evaluates particles with native urine without centrifugation and reports as “counts per μL.” Microscopic examination recommended as the reference method for urine sediment analysis reports results as “counts per HPF (or μL).” Moreover, some results from flow cytometer are needed to be checked visually under microscopy. Therefore, it is worth to establish the consistency of the results from these two methods.MethodsUrine specimens from 412 patients were examined with Sysmex UF‐1000i and manual microscopy using FAST‐READ disposable counting chambers. White blood cell (WBC) and red blood cell (RBC) counting results from UF‐1000i after transferred with the converting factor (0.297) we estimated were compared with that from microscopic examination. Method comparison was performed using Passing‐Bablok analysis.ResultsAfter transferred with the converting factor (0.297), cell counting results from UF‐1000i showed a good correlation with that derived by the reference method (R 2 was 0.868 for RBCs (P < 0.001), 0.882 for WBCs (P < 0.001)). Passing‐Bablok analysis showed no systematic difference (intercept estimate, −1 [95%CI, −7 to 3] and slightly proportional (slope estimate, 1.2 [95%CI, 1.0 to 1.7]) bias between concentrations of cells measured by manual microscopy and Sysmex UF‐1000i using the converting factor.ConclusionThe converting factor (0.297) helps to transfer “counts per μL (non‐centrifugal urine)” to “counts per μL (equal to centrifugal urine),” and to keep the urine particle analysis results of Sysmex UF‐1000i consistent with the results from the reference method.

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L

Gressner¹,

Arndt²

2007

Lexikon Der Medizinischen Laboratoriumsdiagnostik

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Reference Values for Neonates and Children for the UF-100 Urine Flow Cytometer

Cited by 16 publications

References 1 publication

Prevalence of Mycoplasma genitalium in men with urethritis in a large public hospital in Brussels, Belgium: An observational, cross-sectional study

Prevalence of Mycoplasma genitalium in men with urethritis in a large public hospital in Brussels, Belgium: An observational, cross-sectional study

The standardization of the report for urine cell counting—A converting factor for Sysmex UF‐1000i

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