Referenzbereiche von Insulin, insulinähnlichem Wachstumsfaktor 1 (IGF-1) und adrenokortikotropem Hormon bei Ponys

Köller, G.; Bassewitz, K.; Schusser, G. F.

doi:10.15653/tpg-150428

Cited by 7 publications

(5 citation statements)

References 31 publications

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“…Resting insulin can also be measured under forage‐fed conditions, although the effects of different forages and rates of consumption will influence results. In 112 ponies with free access to hay, a reference interval of 2.0‐21.1 μIU/mL was established with the Immulite 2000 chemiluminescent assay . In another study, 6 normal ponies fed 0.25% BM forage as dry matter intake (DMI) had peak insulin values of <3.9 μIU/mL when fed soaked hay and <19.8 μIU/mL when fed dry hay using the same assay (Immulite 2000) .…”

Section: Laboratory Diagnosismentioning

confidence: 99%

ECEIM consensus statement on equine metabolic syndrome

Durham¹,

Frank

McGowan

et al. 2019

Veterinary Internal Medicne

184

227

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Equine metabolic syndrome (EMS) is a widely recognized collection of risk factors for endocrinopathic laminitis. The most important of these risk factors is insulin dysregulation (ID). Clinicians and horse owners must recognize the presence of these risk factors so that they can be targeted and controlled to reduce the risk of laminitis attacks. Diagnosis of EMS is based partly on the horse's history and clinical examination findings, and partly on laboratory testing. Several choices of test exist which examine different facets of ID and other related metabolic disturbances. EMS is controlled mainly by dietary strategies and exercise programs that aim to improve insulin regulation and decrease obesity where present. In some cases, pharmacologic aids might be useful. Management of an EMS case is a long‐term strategy requiring diligence and discipline by the horse's carer and support and guidance from their veterinarians.

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Section: Laboratory Diagnosismentioning

confidence: 99%

ECEIM consensus statement on equine metabolic syndrome

Durham¹,

Frank

McGowan

et al. 2019

Veterinary Internal Medicne

184

227

View full text Add to dashboard Cite

show abstract

“…The diagnosis or exclusion of PPID was based on a combination of clinical signs (hypertrichosis and polydipsia/polyuria) and basal ACTH concentrations using seasonally-adjusted cut-off values of > 27.8 pg/ml in non-autumn months and > 77.4 pg/ml in autumn months [ 33 ]. Adrenocorticotropic hormone levels were determined using the previously validated Immulite 2000 chemiluminescence method [ 34 ] by VetPath Laboratories (Ascot, Western Australia, Australia).…”

Section: Methodsmentioning

confidence: 99%

Metabolic profile distinguishes laminitis-susceptible and -resistant ponies before and after feeding a high sugar diet

Delarocque

Reiche²,

Meier³

et al. 2021

BMC Vet Res

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Background Insulin dysregulation (ID) is a key risk factor for equine endocrinopathic laminitis, but in many cases ID can only be assessed accurately using dynamic tests. The identification of other biomarkers could provide an alternative or adjunct diagnostic method, to allow early intervention before laminitis develops. The present study characterised the metabolome of ponies with varying degrees of ID using basal and postprandial plasma samples obtained during a previous study, which examined the predictive power of blood insulin levels for the development of laminitis, in ponies fed a high-sugar diet. Samples from 10 pre-laminitic (PL – subsequently developed laminitis) and 10 non-laminitic (NL – did not develop laminitis) ponies were used in a targeted metabolomic assay. Differential concentration and pathway analysis were performed using linear models and global tests. Results Significant changes in the concentration of six glycerophospholipids (adj. P ≤ 0.024) and a global enrichment of the glucose-alanine cycle (adj. P = 0.048) were found to characterise the response of PL ponies to the high-sugar diet. In contrast, the metabolites showed no significant association with the presence or absence of pituitary pars intermedia dysfunction in all ponies. Conclusions The present results suggest that ID and laminitis risk are associated with alterations in the glycerophospholipid and glucose metabolism, which may help understand and explain some molecular processes causing or resulting from these conditions. The prognostic value of the identified biomarkers for laminitis remains to be investigated in further metabolomic trials in horses and ponies.

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“…Some studies relied on diagnostic tests, such as the euglycemic hyperinsulinaemic clamp (EHC) [35] or combined glucose-insulin test (CGIT) [36], to detect insulin-resistant horses, while others were conducted prospectively, with laminitis as an outcome [37][38][39]. Another study reported a confidence interval for basal insulin in healthy ponies [41]. It is interesting that the intravenous tests provided lower cut-offs than those obtained from a healthy population, or when subsequent laminitis was considered.…”

Section: Comparison Of Cut-off Valuesmentioning

confidence: 99%

“…Despite all these differences, the published cut-offs for basal insulin values with laminitis as an outcome averaged at around 30 µIU/mL once converted to the Mercodia Equine assay [37][38][39]. The cut-offs targeting insulin resistance or ID were lower at around 10 to 20 µIU/mL (once converted) [34][35][36]41]. This is unsurprising, as the likelihood of laminitis incidence is correlated with the degree of hyperinsulinaemia, meaning that slightly insulin-resistant horses may not develop laminitis at all, even though they remain at risk.…”

Section: Comparison Of Cut-off Valuesmentioning

confidence: 99%

Development of a Web App to Convert Blood Insulin Concentrations among Various Immunoassays Used in Horses

Delarocque,

Feige,

Carslake

et al. 2023

Animals

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The measurement of the blood insulin concentration, and comparison to cut-offs, is essential in diagnosing insulin dysregulation, a common equine endocrinopathy. However, different insulin assays provide disparate results. We aimed to ease comparison between assays by compiling original and published data into a web app to convert insulin measurements from one assay to another. Data were available for ADVIA Centaur insulin chemiluminescent immunoassay (CLIA), Beckman Coulter insulin radioimmunoassay (RIA), Immulite 1000 CLIA, Immulite 2000 CLIA, Immulite 2000 XPi CLIA, Mercodia equine insulin enzyme-linked immunosorbent assay (ELISA), and Millipore porcine insulin RIA. Linear models were fitted for 13 assay pairs using non-decreasing splines, and integrated into this app. Assay comparisons including data from several studies showed a lower performance. This indicates technical variation between laboratories, which has not been described before, but is relevant when diagnostic measurements and cut-offs are provided by different laboratories. Nevertheless, the models’ overall high performance (median r2 = 0.94; range 0.57–1.00) supports their use to interpret results from diagnostic insulin measurements when the reference assay is unavailable, and to compare values obtained from different assays.

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Referenzbereiche von Insulin, insulinähnlichem Wachstumsfaktor 1 (IGF-1) und adrenokortikotropem Hormon bei Ponys

Abstract: The calculated reference ranges of insulin and ACTH are helpful for the diagnosis and clinical monitoring of equine metabolic syndrome and pituitary pars intermedia dysfunction (PPID).

Cited by 7 publications

References 31 publications

ECEIM consensus statement on equine metabolic syndrome

ECEIM consensus statement on equine metabolic syndrome

Metabolic profile distinguishes laminitis-susceptible and -resistant ponies before and after feeding a high sugar diet

Development of a Web App to Convert Blood Insulin Concentrations among Various Immunoassays Used in Horses

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