Referenzwerte für die Bestimmungen der Transaminasen GOT und GPT sowie der alkalischen Phosphatase im Serum mit optimierten Standardmethoden

Thefeld, Wolfgang; Hoffmeister, H. E.; Busch, Eskild; Koller, P. U.; Vollmar, Jens

doi:10.1055/s-0028-1107760

Cited by 78 publications

(25 citation statements)

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“…Serum alanine transaminase (ALT) and alkaline phosphatase (ALP) were estimated spectrophotometrically according to the method of Thefeld et al [27] and Schlebusch et al [28], respectively, using Human Kit (Germany) according to the instruction of the supplier. Serum aspartate aminotransferase (AST) was estimated spectrophotometrically according to the method of Thefeld et al [27] using Swemed Diagnostics kit (India).…”

Section: Methodsmentioning

confidence: 99%

Comparison between the Hypolipidemic Activity of Parsley and Carob in Hypercholesterolemic Male Rats

Rabey

Al‐Seeni

Al-Ghamdi

2017

BioMed Research International

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Hypercholesterolemia is commonly associated with obesity that leads to heart diseases and diabetes. The hepatocardioprotective activity of parsley and carob methanol extract was tested in hypercholesterolemic male rats. Twenty-four male albino rats were divided into four groups (n = 6). Group 1 was the negative control group fed with fat rich diet, group 2 (G2) was hypercholesterolemic rats fed with fat rich diet with 2% cholesterol, and group 3 and group 4 (G3 and G4) were hypercholesterolemic rats supplemented with 2% cholesterol and cotreated with 20% w/w parsley seed methanol extract and 20% w/w carob legume methanol extract, respectively. The experiment was conducted for eight weeks. The positive hypercholesterolemic rats showed significant increase in serum levels of total cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), lactate dehydrogenase (LDH), creatine kinase-mb, liver function enzymes, and decrease in the high density lipoproteins (HDL). Moreover, heart and liver tissues were ameliorated and nearly restored their normal appearance. It could be concluded that both parsley and carob extracts supplementations have a protective effect against hyperlipidemia and improved the histological alteration in heart and liver tissues. The methanol extract of parsley appeared to be more efficient than that of carob in lowering hypercholesterolemia.

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Section: Methodsmentioning

confidence: 99%

Comparison between the Hypolipidemic Activity of Parsley and Carob in Hypercholesterolemic Male Rats

Rabey

Al‐Seeni

Al-Ghamdi

2017

BioMed Research International

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“…Serum creatinine was estimated by the alkaline picrate method (Toro and Ackerman 1975) whereas BUN was estimated by a modified Berthelot method using auto analyzer slim (SEAC; Labnovation, China). SEAC was used to estimate SGPT and SGOT concentrations in blood (Thefeld et al 1974). Serum calcium was estimated by the Arsenazo III method (Bauer 1981) while the blood serum albumin was estimated by bromocresol green (BCG) method using auto analyzer slim (SEAC).…”

Section: Methodsmentioning

confidence: 99%

Influence of mercury from fly ash on cattle reared nearby thermal power plant

Mahajan¹,

Yadav

Dakshinkar³

et al. 2012

Environ Monit Assess

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Cattle grazing nearby coal-fired power stations are exposed to fly ash. The present investigation aims to assess the environmental and health impacts of fly ash containing mercury emitted from thermal power plant. The health effect of fly ash were studied using 20 lactating cattle reared within a 5-km radius of s thermal power plant for the possible effect of fly ash such as the alterations in hematological and biochemical parameters of blood, milk, and urine. Results indicated that the hemoglobin levels (6.65 ± 0.40 g/dl) were significantly reduced in all the exposed animals. Biochemical parameters viz., blood urea nitrogen (27.35 ± 1.19 mg/dl), serum glutamate oxaloacetate transaminase (43.39 ± 3.08 IU/l), albumin, and creatinine were found to be increased, whereas serum glutamate pyruvic transaminase (29.26 ± 2.02) and Ca(2+) were observed to be statistically insignificant in exposed animals. Mercury concentrations estimated in the blood, milk, and urine of exposed (n = 20) and control (n = 20) animals were 7.41 ± 0.86, 4.75 ± 0.57, 2.08 ± 0.18, and 1.05 ± 0.07, 0.54 ± 0.03, 0.20 ± 0.02 μg/kg, respectively. The significant increase (P < 0.01) in the levels of mercury in blood, milk, and urine of exposed animals in comparison to control indicated that the alterations of biochemical parameters in exposed cattle could be due to their long term exposure to fly ash mercury which may have direct or indirect impact on human populations via food chain.

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“…Einmal ist die Auswahl der Personen relativ klein und zum anderen konnte bei der Auswahl der Individuen keine so große Sorgfalt angewandt werden, wie sie für die Aufstellung von Normalbereichen notwendig gewesen wäre. Vergleicht man unsere Werte mit denen, die kürzlich in der Deutschen Medizinischen Wochenschrift (14) veröffentlicht wurden, so können wir aber dennoch sägen, daß das Ergebnis als Vergleichsuntersuchung durchaus zu akzeptieren ist.…”

Section: Blutentnahmeunclassified

Enzymaktivitäten im Serum und Plasma von Mensch, Hund und Ratte sowie deren Veränderungen beim Aufbewahren des Blutes

Breuer¹,

Stucky²

1975

Clinical Chemistry and Laboratory Medicine

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Enzyme-activities in serum and plasma of man, dog and rat, and the variation of these enzyme activities during storage of the bloodSummary: The following enzymes were determined in the serum and plasma of man, dog and rat: alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase and -hydroxybutyrate dehydrogenase. The enzyme assays were performed on an Eppendorf-Enzymautomat 5010 using optimised conditions at 25 °C. The enzyme-activities changed by variable amounts during standing of the blood. This concerned mainly lactate dehydrogenase, -hydroxybutyrate dehydrogenase and aspartate aminotransferase in the serum of the rat. In human serum and in dog serum, and in the plasma of man, dog and rat this effect was only less pronounced.The following laboratory reference ranges were determined: alanine aminotransferase and alkaline phosphatase in the serum of dog and rat, and lactate dehydrogenase and aspartate aminotransferase in the serum of dog according to age and sex. In comparison these ranges were determined in a group of healthy volunteers. The distribution of the data was examined.

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Referenzwerte für die Bestimmungen der Transaminasen GOT und GPT sowie der alkalischen Phosphatase im Serum mit optimierten Standardmethoden

Cited by 78 publications

References 0 publications

Comparison between the Hypolipidemic Activity of Parsley and Carob in Hypercholesterolemic Male Rats

Comparison between the Hypolipidemic Activity of Parsley and Carob in Hypercholesterolemic Male Rats

Influence of mercury from fly ash on cattle reared nearby thermal power plant

Enzymaktivitäten im Serum und Plasma von Mensch, Hund und Ratte sowie deren Veränderungen beim Aufbewahren des Blutes

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