1999
DOI: 10.1006/jmbi.1998.2378
|View full text |Cite
|
Sign up to set email alerts
|

Refined Crystal Structures of Native Human Angiogenin and Two Active Site Variants: Implications for the Unique Functional Properties of an Enzyme Involved in Neovascularisation During Tumour Growth

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

3
157
2
8

Year Published

2000
2000
2021
2021

Publication Types

Select...
9

Relationship

2
7

Authors

Journals

citations
Cited by 95 publications
(170 citation statements)
references
References 112 publications
3
157
2
8
Order By: Relevance
“…Angiogenin was claimed to specifically cleave tRNAs. But, neither a specific cleavage site nor a specific tRNA species as a substrate has been reported (28), which is in clear contrast to colicins D and E5.…”
Section: Discussionmentioning
confidence: 87%
“…Angiogenin was claimed to specifically cleave tRNAs. But, neither a specific cleavage site nor a specific tRNA species as a substrate has been reported (28), which is in clear contrast to colicins D and E5.…”
Section: Discussionmentioning
confidence: 87%
“…4 and 11). The importance of such selective amino acid residue mediated path for different mutants is supported by published crystal structure of ANG, which established Leu115 as a factor responsible for weak ribonucleolytic activity [21]. Mutants V113I, R121C, R121H, K54E, F100I, A98V and P123L, which do not show any conformational switching of His114, exhibited no hydrogen bond interaction path from the site of mutation to His114.…”
Section: Resultsmentioning
confidence: 94%
“…Crystal structure of human angiogenin (PDB: 1B1I) was used as the starting structure and desired residues were mutated in silico keeping its secondary structure intact [21]. Heteroatoms, cofactor CIT and crystallographic water were removed a priori.…”
Section: Generalized Born Solvent Molecular Simulationsmentioning
confidence: 99%
“…Crystal structures of ANG show that Gln12 forms part of the P 1 subsite where phosphodiester bond cleavage occurs; it makes hydrogen bonds with Lys40 (32) and, in the complex of ANG with phosphate, with ligand as well (46). However, previous studies on this residue (47) and on its counterpart Gln11 in RNase A (48) have shown that replacement by Ala does not cause any significant loss of activity, which therefore indicates that Gln12/Gln11 is not a critical residue.…”
Section: Discussionmentioning
confidence: 99%