Refined definition of the critical micelle concentration and application to alkyl maltosides used in membrane protein research

Bothe, Adrian; Zouni, Athina; Müh, Frank

doi:10.1039/d2ra07440k

Cited by 4 publications

(15 citation statements)

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“…More similar to detergents are n-alkyl alcohols with the number of carbon atoms between 1 and 5, which were investigated in aqueous biphasic systems composed of PEG and salts (Willauer et al 2002). The authors characterized the partitioning of the alcohols between the two phases by the transfer free energy of a methylene group, ΔG CH 2 , a ( ) measure of solution polarity, which is similar to the strategy employed in molecular thermodynamic modeling of g mic (Nagarajan andRuckenstein 1991h et al 2015;Bothe et al 2023). Willauer et al (2002) found a linear correlation between ΔG CH 2 and the difference in c OE between the two phases that is independent of the relative molecular weight (in this case, PEG1000, PEG2000, and PEG3400 were investigated).…”

Section: Discussionmentioning

confidence: 99%

“…( 27) is too complicated for practical use. However, Bothe et al (2023) found that for large m, x ≈ y at the breaking point corresponding to the traditional de nition of the CMC. This result also follows from Eq.…”

Section: Note Thatmentioning

confidence: 98%

“…For X P = 0, Eq. ( 16) is the same micellar size distribution as given by Bothe et al (2023). For X P > 0, Eq.…”

Section: Note Thatmentioning

confidence: 98%

“…Note that N ν is the number of micelles of size ν. Then, the total particle number is N tot = N W + N det + N P + N cos (5) and we can de ne the mole fractions for all species except detergent as…”

Section: Thermodynamics Of Micelle Formationmentioning

confidence: 99%

“…Above the CMC, the detergent molecules aggregate into micelles (Wennerström and Lindman 1979; Nagarajan and Ruckenstein 1991; Chakraborty and Ghosh 2008; Dey et al 2021) and also form belts surrounding the hydrophobic protein parts (Müh and Zouni 2008). The CMC is very sensitive to co-solutes in the buffer ( In the framework of our long-term research aiming at a better understanding of the physical chemistry underlying the crystallization process Zouni 2008h et al 2015;Bothe et al 2023), we investigated the in uence of PEG polymers of various molecular sizes on the micelle formation of alkyl maltosides, a class of detergents used in PSII research. In our contribution, we describe the results of these experiments alongside an analysis based on thermodynamic theory.…”

Section: Introductionmentioning

confidence: 99%

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Towards understanding the crystallization of photosystem II: Influence of poly(ethylene glycol) of various molecular sizes on the micelle formation of alkyl maltosides

Müh,

Bothe,

Zouni

2023

Preprint

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The influence of poly(ethylene glycol) (PEG) polymers H-(O-CH2-CH2)p-OH with different average molecular sizes p on the micelle formation of n-alkyl-β-D-maltoside detergents with the number of carbon atoms in the alkyl chain ranging from 10 to 12 is investigated with the aim to learn more about the detergent behavior under conditions suitable for the crystallization of the photosynthetic pigment-protein complex photosystem II. PEG is shown to increase the critical micelle concentration (CMC) of all three detergents in the crystallization buffer in a way that the free energy of micelle formation increases linearly with the concentration of oxyethylene units (O-CH2-CH2) irrespective of the actual molecular weight of the polymer. The CMC shift is modeled by assuming for simplicity that it is dominated by the interaction between PEG and detergent monomers and is interpreted in terms of an increase of the transfer free energy of a methylene group of the alkyl chain by 0.2 kJ mol–1 per 1 mol L–1 increase of the concentration of oxyethylene units at 298 K. Implications of this effect for the solubilization and crystallization of protein-detergent complexes as well as detergent extraction from crystals are discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Note Thatmentioning

confidence: 98%

“…For X P = 0, Eq. ( 16) is the same micellar size distribution as given by Bothe et al (2023). For X P > 0, Eq.…”

Section: Note Thatmentioning

confidence: 98%

Section: Thermodynamics Of Micelle Formationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Towards understanding the crystallization of photosystem II: Influence of poly(ethylene glycol) of various molecular sizes on the micelle formation of alkyl maltosides

Müh,

Bothe,

Zouni

2023

Preprint

Self Cite

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Towards understanding the crystallization of photosystem II: influence of poly(ethylene glycol) of various molecular sizes on the micelle formation of alkyl maltosides

Müh,

Bothe,

Zouni

2024

Photosynth Res

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The influence of poly(ethylene glycol) (PEG) polymers H–(O–CH2–CH2)p–OH with different average molecular sizes $$p$$ p on the micelle formation of n-alkyl-β-D-maltoside detergents with the number of carbon atoms in the alkyl chain ranging from $$10$$ 10 to $$12$$ 12 is investigated with the aim to learn more about the detergent behavior under conditions suitable for the crystallization of the photosynthetic pigment–protein complex photosystem II. PEG is shown to increase the critical micelle concentration (CMC) of all three detergents in the crystallization buffer in a way that the free energy of micelle formation increases linearly with the concentration of oxyethylene units (O–CH2–CH2) irrespective of the actual molecular weight of the polymer. The CMC shift is modeled by assuming for simplicity that it is dominated by the interaction between PEG and detergent monomers and is interpreted in terms of an increase of the transfer free energy of a methylene group of the alkyl chain by 0.2 kJ mol−1 per 1 mol L−1 increase of the concentration of oxyethylene units at 298 K. Implications of this effect for the solubilization and crystallization of protein–detergent complexes as well as detergent extraction from crystals are discussed.

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Recent advances in the epithelial barrier theory

Pat,

Yazici,

D’Avino

et al. 2024

International Immunology

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The epithelial barrier theory links the recent rise in chronic non-communicable diseases, notably autoimmune and allergic disorders, to environmental agents disrupting the epithelial barrier. Global pollution and environmental toxic agent exposure have worsened over six decades because of uncontrolled growth, modernisation, and industrialisation, affecting human health. Introducing new chemicals without any reasonable control of their health effects through these years has led to documented adverse effects, especially on the skin and mucosal epithelial barriers. These substances, such as particulate matter, detergents, surfactants, food emulsifiers, micro- and nano-plastics, diesel exhaust, cigarette smoke and ozone, have been shown to compromise the epithelial barrier integrity. This disruption is linked to the opening of the tight junction barriers, inflammation, cell death, oxidative stress and metabolic regulation. Consideration must be given to the interplay of toxic substances, underlying inflammatory diseases, and medications, especially in affected tissues. This review article discusses the detrimental effect of environmental barrier-damaging compounds on human health and involves cellular and molecular mechanisms.

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Refined definition of the critical micelle concentration and application to alkyl maltosides used in membrane protein research

Abstract: A refined definition of the critical micelle concentration is connected to fluorescence methods and applied to test a molecular thermodynamic model of nonionic detergents used for in vitro studies of membrane proteins.

Cited by 4 publications

References 76 publications

Towards understanding the crystallization of photosystem II: Influence of poly(ethylene glycol) of various molecular sizes on the micelle formation of alkyl maltosides

Towards understanding the crystallization of photosystem II: Influence of poly(ethylene glycol) of various molecular sizes on the micelle formation of alkyl maltosides

Towards understanding the crystallization of photosystem II: influence of poly(ethylene glycol) of various molecular sizes on the micelle formation of alkyl maltosides

Recent advances in the epithelial barrier theory

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