Refined polysaccharide from Dendrobium devonianum resists H1N1 influenza viral infection in mice by activating immunity through the TLR4/MyD88/NF-κB pathway

Abstract

Dendrobium polysaccharide exhibits multiple biological activities, such as immune regulation, antioxidation, and antitumor. However, its resistance to viral infection by stimulating immunity is rarely reported. In this study, we explored the effect and mechanism of DVP-1, a novel polysaccharide from Dendrobium devonianum, in the activation of immunity. After being activated by DVP-1, the ability of mice to prevent H1N1 influenza virus infection was investigated. Results of immune regulation showed that DVP-1 significantly improved the immune organ index, lymphocyte proliferation, and mRNA expression level of cytokines, such as IL-1β, IL-4, IL-6, and TNF-α in the spleen. Immunohistochemical results showed that DVP-1 obviously promoted the mucosal immunity in the jejunum tissue. In addition, the expression levels of TLR4, MyD88, and TRAF6 and the phosphorylation levels of TAK1, Erk, JNK, and NF-κB in the spleen were upregulated by DVP-1. The virus infection results showed that the weight loss of mice slowed down, the survival rate increased, the organ index of the lung reduced, and the virus content in the lung decreased after DVP-1 activated immunity. By activating immunity with DVP-1, the production of inflammatory cells and inflammatory factors in BALF, and alveolar as well as peribronchiolar inflammation could be prevented. The results manifested that DVP-1 could resist H1N1 influenza virus infection by activating immunity through the TLR4/MyD88/NF-κB pathway.

Intestinal microbiota analysis and network pharmacology reveal the mechanism by which Lianhua Qingwen capsule improves the immune function of mice infected with influenza A virus

“…Animal experiments have also confirmed that LHQW can reduce the viral load in the lungs of mice with viral pneumonia, reduce the expression of inflammatory factors in the lungs, and improve lung injury (Xia et al, 2020;Su et al, 2022). It is well known that viral clearance in the lung depends mainly on lymphocytes and macrophages (Bedi et al, 2022;Cammann et al, 2022;Harris and Borg, 2022;McGee et al, 2022;Verma et al, 2022;Wei et al, 2022;Zhang H. et al, 2022;Zhang M. et al, 2022); however, the present findings do not clarify how LHQW reduces the viral load in the lung and improves lung injury with viral pneumonia.…”

“…The blood on the surface was absorbed with filter paper, and the changes in lung organ index were weighed and recorded using an analytical balance. The formulae used in this study were A = B/C*100%, where A represents the lung organ index, B represents the lung weight (mg), and C represents the body weight (g); and D = (E − F)/G*100%, where D represents the inhibition rate of the lung index (%), E represents the average lung index of the infected group, F represents the average lung index of the administration group, and G represents the average lung index of the infected group (Han et al, 2021;Song et al, 2021;Li-Juan et al, 2022;Wei et al, 2022).…”

The Polysaccharide from Dendrobium officinale Can Improve Mice with Sjögren’s Syndrome by Regulating BAFF and Fas Expressions

“…DOP, the primary active component of D. ofcinale, is widely recognized as an immune-boosting medication due to its traditional tonic properties, supported by a plethora of literature. However, according to this experiment results, DOP not only has immune-enhancing efects [9,52] but also inhibits proinfammatory factors and apoptosis via diferent pathways in a mouse model of hyperimmune ESS, which provides a rationale for the role of DOP with bidirectional immunomodulatory functions. Additionally, our laboratory will further investigate the BAFF/ BAFFR and Fas/Fas-L signaling pathways, as well as the interaction between these two pathways, in the context of the ESS mice experiment in which DOP treatment reduces infammation and apoptosis.…”

“…Treatments are designed to enhance the gland secretion in the mouth, nose, and eyes, ultimately strengthening the body's ability to fght against illnesses [8]. Recent studies have indicated that natural medicines or active components exhibit positive outcomes in immunomodulation [9], reducing infammation [2] and anti-apoptosis [10] while posing minimal side efects.…”

Anti-inflammatory compounds in human peripheral blood mononuclear cells from dendrobium species

“…For instance, polysaccharides from Dendrobium officinale Kimura et Migo and water extracts from Dendrobium thyrsiflorum B.S.W illiam s showed im m une m odulatory effects in THP-1 and RAW 264.7 macrophage cells, respectively (Qiang et al, 2018;. Moreover, the potent immunomodulatory constituents such as polysaccharides from Dendrobium devonianum and D. officinale have been reported in the mice models (Sun et al, 2022;Wei et al, 2022;Xie et al, 2022). Particularly, a bibenzyl derivative, 4,5-dihydroxy-3,3´,4´-trimethoxybibenzyl, from Dendrobium lindleyi Steud.…”