Refinement of the Listeria monocytogenes σB regulon through quantitative proteomic analysis

Mujahid, Sana; Orsi, Renato H.; Vangay, Pajau; Boor, Kathryn J.; Wiedmann, Martin

doi:10.1099/mic.0.066001-0

Cited by 27 publications

(23 citation statements)

References 42 publications

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“…In addition, 12 of the 29 proteins that were found to be positively regulated in the parent strain, were also found to be positively regulated by σ B in a recent proteomics study, which compared L . monocytogenes parent strain 10403S and Δ sigB mutant grown to stationary phase under the same conditions as used here [23]. While these 12 proteins likely represent proteins that are positively regulated by σ B , the other 17 proteins that showed higher levels in the parent strain as compared to the Δ BCHL strain, but were not identified as positively regulated by any of the alternative σ factors, represent candidate proteins for redundant co-regulation by multiple alternative σ factors.…”

Section: Resultsmentioning

confidence: 99%

“…Twelve of the 29 proteins that were found to be positively regulated in the parent strain were also found to be positively regulated by σ B in a recent proteomics study, which compared L . monocytogenes parent strain 10403S and a Δ sigB mutant [23]; these proteins include Lmo2748, Lmo2213, Lmo2158, Lmo2047, Lmo1830, Lmo0913, Lmo0796, Lmo0794, Lmo0722, Lmo0654, Lmo0539, and Lmo0265. The 17 proteins that show higher levels in the parent strain as compared to the Δ BCHL strain, but were not identified as positively regulated by any of the alternative σ factors include Lmo1540, Lmo2610, Lmo1422, Lmo1421, Lmo1602, Lmo1426, Lmo1428, Lmo2205, Lmo2398, Lmo1601, Lmo0554, Lmo1634, Lmo0110, Lmo2558, Lmo0783, Lmo0134, and Lmo0098.…”

Section: Resultsmentioning

confidence: 99%

“…[23]; this study compared protein levels between the 10403S parent strain and an isogenic Δ sigB strain.…”

Section: Resultsmentioning

confidence: 99%

“…We particularly focused on exploring the contributions of these alternative σ factors to regulating protein production as, despite availability of a number of proteomics data sets on the σ B regulon [15,16], only a single proteomics study on the σ L regulon is available [22]. While alternative σ factors directly regulate transcription of genes, it is increasingly clear that alternative σ factors also make important indirect contributions to protein production via mechanisms other than transcriptional activation of a σ factor dependent promoter upstream of a protein encoding gene, including through regulation of non-coding RNAs or through direct transcriptional up-regulation of a protein that in turn, directly or indirectly, affects production of other proteins [23]. The goal of this proteomics study was thus to specifically identify additional post-transcriptional regulatory pathways that are linked to the action of alternative σ factors in L .…”

Section: Introductionmentioning

confidence: 99%

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Protein level identification of the Listeria monocytogenes Sigma H, Sigma L, and Sigma C regulons

et al. 2013

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BackgroundTranscriptional regulation by alternative sigma (σ) factors represents an important mechanism that allows bacteria to rapidly regulate transcript and protein levels in response to changing environmental conditions. While the role of the alternative σ factor σB has been comparatively well characterized in L. monocytogenes, our understanding of the roles of the three other L. monocytogenes alternative σ factors is still limited. In this study, we employed a quantitative proteomics approach using Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) to characterize the L. monocytogenes σL, σH, and σC protein regulons. Proteomic comparisons used a quadruple alternative σ factor mutant strain (ΔBCHL) and strains expressing a single alternative σ factor (i.e., σL, σH, and σC; strains ΔBCH, ΔBCL, and ΔBHL) to eliminate potential redundancies between σ factors.ResultsAmong the three alternative σ factors studied here, σH provides positive regulation for the largest number of proteins, consistent with previous transcriptomic studies, while σL appears to contribute to negative regulation of a number of proteins. σC was found to regulate a small number of proteins in L. monocytogenes grown to stationary phase at 37°C. Proteins identified as being regulated by multiple alternative σ factors include MptA, which is a component of a PTS system with a potential role in regulation of PrfA activity.ConclusionsThis study provides initial insights into global regulation of protein production by the L. monocytogenes alternative σ factors σL, σH, and σC. While, among these σ factors, σH appears to positively regulate the largest number of proteins, we also identified PTS systems that appear to be co-regulated by multiple alternative σ factors. Future studies should not only explore potential roles of alternative σ factors in activating a “cascade” of PTS systems that potentially regulate PrfA, but also may want to explore the σL and σC regulons under different environmental conditions to identify conditions where these σ factors may regulate larger numbers of proteins or genes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…[23]; this study compared protein levels between the 10403S parent strain and an isogenic Δ sigB strain.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Protein level identification of the Listeria monocytogenes Sigma H, Sigma L, and Sigma C regulons

et al. 2013

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“…Expression of 17 proteins involved in regulation of metabolism and stress-related functions was found to be upregulated by σ B (Abram et al, 2008). This study indicated that σ B positively regulated pathways pertaining to uptake and biosynthesis of glycine and also energy metabolism (Mujahid et al, 2013). Another proteomic study compared proteomes of the parent strain 10403S and an isogenic ΔsigB mutant in combination with meta-analysis of earlier published microarray (Oliver et al, 2010;Ollinger et al, 2009;Raengpradub et al, 2008) and RNASeq (Oliver et al, 2009) data.…”

Section: Proteome Of Listeriamentioning

confidence: 85%