Reflectance confocal‐laser‐scanning microscopy in vivo assessments of cigarette‐induced dynamic alterations of cutaneous microcirculation on histomorphological level

Altintas, M.A.; Altıntaş, Ahmet; Guggenheim, Merlin; Gohritz, Andreas; Meyer-Marcotty, Max; Vogt, Peter M.

doi:10.1002/jemt.20661

Cited by 12 publications

(12 citation statements)

References 18 publications

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“…However, comorbid factors especially a history of smoking, play an important role in relation with cutaneous microcirculation. We confirmed this in previous studies using in vivo RMCM (Altintas et al,2008a). Indeed, this could have been contributing to the contrary results of studies on cutaneous microcirculation, as a smoking history and other comorbidities were not fully considered as influencing factors (Goldberg et al,1990; Orenstein et al,1988; Park et al,1997).…”

Section: Discussionsupporting

confidence: 91%

“…Reflectance‐Mode‐Confocal‐Microscopy (RMCM) is a high‐resolution imaging technique to noninvasively investigate human skin in vivo and in real‐time. Using this technique, detailed evaluation and investigation of the histomorphology and microcirculation of the skin was carried out and published (Altintas et al,2008a; Gonzalez et al,2004; Rajadhyaksha et al,1999). In earlier studies, we could show disturbances of arterial and venous perfusion in free musculocutaneous latissimus dorsi flaps (Altintas et al,2008b).…”

Section: Introductionmentioning

confidence: 99%

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In vivo comparison of microcirculation and histomorphology of two different flap donor sites

Altıntaş

Stasch

Oezcelik

et al. 2011

Microscopy Res & Technique

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

In vivo comparison of microcirculation and histomorphology of two different flap donor sites

Altıntaş

Stasch

Oezcelik

et al. 2011

Microscopy Res & Technique

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“…This application is known as "confocal laser-scanning capillaroscopy" and it has been used to assess and quantify the morphological and dynamic changes of microcirculation occurring in normal skin under external stress conditions, such as cigarette smoking [26] and environmental temperature [27]. In addition, previous ex vivo [28] and in vivo [21] RCM studies have preliminarily described morphological changes of the epidermis, dermis and capillaries of the upper dermis in SSc patients.…”

Section: Discussionmentioning

confidence: 99%

In vivo reflectance confocal microscopy features of cutaneous microcirculation and epidermal and dermal changes in diffuse systemic sclerosis and correlation with histological and videocapillaroscopic findings

Venturini

Arisi

Zanca

et al. 2014

European Journal of Dermatology

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349 To cite this article: Venturini M, Arisi M, Zanca A, Cavazzana I, Gonzàlez S, Franceschini F, Calzavara-Pinton P. In vivo reflectance confocal microscopy features of cutaneous microcirculation and epidermal and dermal changes in diffuse systemic sclerosis and correlation with histological and videocapillaroscopic findings. Eur J Dermatol 2014; 24(3): 349-55 In vivo reflectance confocal microscopy features of cutaneous microcirculation and epidermal and dermal changes in diffuse systemic sclerosis and correlation with histological and videocapillaroscopic findingsBackground: Videocapillaroscopy of the nail fold is the current gold standard to assess progressive changes of the capillary network in patients with systemic sclerosis (SSc). Reflectance confocal microscopy (RCM) is a non-invasive optical imaging tool that allows in vivo visualization of the skin structures and cutaneous microcirculation. Objective: To investigate qualitative and quantitative changes of the cutaneous microcirculation and dermal-epidermal alterations of SSc patients by RCM and to correlate the images with findings of videocapillaroscopy and histology. Methods: Ten patients affected by diffuse-type SSc with skin involvement and 10 healthy controls were enrolled. All subjects underwent RCM of the dorsal and ventral surfaces of the middle third of the left forearm and nailfold videocapillaroscopy. Skin biopsies for histological and immunohistochemical investigations were taken from 5 patients and 2 healthy controls. Results: At RCM observation, the diameter, perimeter and area of cutaneous capillaries were significantly increased in comparison to healthy controls, as histologically confirmed, whereas blood flow speed was significantly slower. Videocapillaroscopy showed a pathologic pattern of disease activity in 8 SSc patients and was non-specific in the remaining 2. In addition, RCM showed epidermal atrophy, flattening of rete ridges and dermal fibrosis in 7 SSc patients with long-standing disease but not in 3 patients with a recent onset. Conclusion: RCM provides measurable morphological and functional findings of microcirculation in patients suffering from diffuse-type SSc. These findings can integrate with, but not substitute, those provided by standard videocapillaroscopy. In addition, unlike videocapillaroscopy, RCM allows the investigation of epidermal and dermal changes.

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“…It seems that the advent of non‐invasive imaging techniques has not yet reached its full potential and more approaches and applications are still being described. Very recently, a study of the dynamic properties of capillaries influenced by nicotine was published (10), which underlines the increased interest in capillary imaging. The use of CLSC will hopefully find its place in the increasing armamentarium of diagnostic procedures that are available to the dermatologist.…”

Section: Discussionmentioning

confidence: 99%

Confocal laser‐scanning capillaroscopy: a novel approach to the analysis of skin capillaries in vivo

Hegyi¹,

Hegyi²,

Messer³

et al. 2009

Skin Research and Technology

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This new approach offers a considerable advantage compared with nailfold capillaroscopy, which can only be performed at the proximal nail segment, and over histological analysis, which can be hampered by fixation artifacts resulting in altered size and shape of the vessels to be analyzed. CLSC could allow for precise analysis of in vivo skin vasculature in systemic and proliferative diseases of the skin.

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Reflectance confocal‐laser‐scanning microscopy in vivo assessments of cigarette‐induced dynamic alterations of cutaneous microcirculation on histomorphological level

Abstract: Reflectance CLSM enables qualitative and quantitative observation of dynamic processes of cutaneous microcirculation on histomorphological level.

Cited by 12 publications

References 18 publications

In vivo comparison of microcirculation and histomorphology of two different flap donor sites

In vivo comparison of microcirculation and histomorphology of two different flap donor sites

In vivo reflectance confocal microscopy features of cutaneous microcirculation and epidermal and dermal changes in diffuse systemic sclerosis and correlation with histological and videocapillaroscopic findings

Confocal laser‐scanning capillaroscopy: a novel approach to the analysis of skin capillaries in vivo

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