Reflectance Confocal Microscopic Features of Dermatophytes, Scabies, and &lt;emph type="ital"&gt;Demodex&lt;/emph&gt;

Slutsky, Jordan B.; Rabinovitz, Harold; Grichnik, James M.; Marghoob, Ashfaq A.

doi:10.1001/archdermatol.2011.193

Cited by 35 publications

(28 citation statements)

References 2 publications

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“…In the en-face mode, the mites present as prominent bright round dots within the otherwise dark follicle, comparable to the horizontal mode of imaging used in reflectance confocal microscopy (RCM), which we and others have studied for the visualization and quantification of demodex mites in vivo [25,26,27]. In this study, using HD-OCT, demodex mites were found to a far lesser extent in the controls; this is in contrast to our experience with RCM, where the mites were also present in the controls with a mean number of 22.4 in the 5 × 5 mm scanning area.…”

Section: Discussionmentioning

confidence: 93%

High-Definition Optical Coherence Tomography for the in vivo Detection of Demodex Mites

et al. 2012

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Background: Demodex mites are involved in different skin diseases and are commonly detected by skin scrape tests or superficial biopsies. A new high-definition optical coherence tomography (HD-OCT) with high lateral and axial resolution in a horizontal (en-face) and vertical (slice) imaging mode might offer the possibility of noninvasive and fast in vivo examination of demodex mites. Methods: Twenty patients with demodex-related skin diseases and 20 age- and gender-matched healthy controls were examined by HD-OCT. Mites per follicle and follicles per field of view were counted and compared to skin scrape tests. Results: HD-OCT images depicted mites in the en-face mode as bright round dots in groups of 3–5 mites per hair follicle. In the patients with demodex-related disease, a mean number of 3.4 mites per follicle were detected with a mean number of 2.9 infested follicles per area of view compared to a mean of 0.6 mites in 0.4 infested follicles in the controls. The skin scrape tests were negative in 21% of the patients. Conclusion: The innovative HD-OCT enables fast and noninvasive in vivo recognition of demodex mites and might become a useful tool in the diagnosis and treatment monitoring of demodex-related skin diseases.

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Section: Discussionmentioning

confidence: 93%

High-Definition Optical Coherence Tomography for the in vivo Detection of Demodex Mites

et al. 2012

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“…It has been proved to be useful in the assessment of skin tumors and inflammatory conditions [12,13]. This technique also offers the capability of characterizing cutaneous viral and fungal infections as well as parasitic infestations [14,15,16]. The presence and morphology of Demodex folliculorum [17], RCM images with histopathologic correlation of molluscum contagiosum [18], branching fungal hyphae, diagnosis of tinea corporis and Sarcoptes scabiei eggs with developing mites and feces [14], among others, have been described with this tool.…”

Section: Review Of the Literature And Discussionmentioning

confidence: 99%

“…This technique also offers the capability of characterizing cutaneous viral and fungal infections as well as parasitic infestations [14,15,16]. The presence and morphology of Demodex folliculorum [17], RCM images with histopathologic correlation of molluscum contagiosum [18], branching fungal hyphae, diagnosis of tinea corporis and Sarcoptes scabiei eggs with developing mites and feces [14], among others, have been described with this tool. Interestingly, RCM has proved to be a noninvasive, real-time tool for the in vivo imaging of the intracellular organism Treponema pallidum in skin lesions of secondary syphilis [16], disclosing small elongated bright particles with a spiral shape intermingled with the keratinocytes of the stratum granulosum, stratum spinosum and basal layers.…”

Section: Review Of the Literature And Discussionmentioning

confidence: 99%

In vivo Confocal Microscopy Features of Cutaneous Leishmaniasis

et al. 2014

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Background: Cutaneous leishmaniasis is widely distributed, Spain being a hypoendemic region. Noninvasive bedside detection of the histopathologic response to the intracellular organism that allows rapid diagnosis and prompt therapy could be the ideal tool to manage a commonly self-healing lesion. Confocal microscopy is a technique which allows in vivo examination of the skin at cellular resolution. Methods: We describe the in vivo confocal microscopic features of cutaneous leishmaniasis, finding a correlation with dermoscopy and histopathology. Conclusions and Relevance: This case illustrates the capability of confocal microscopy to characterize the cutaneous infection by Leishmania organisms and to perform a noninvasive diagnosis.

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“…CM offers sufficient penetration depth and better resolution compared to OCT, which means that the mite, specific structures of the mite and the surrounding skin are more clearly imaged [2, 10, 11, 12]. Furthermore, the technique offers the possibility of video recording [11].…”

Section: Discussionmentioning

confidence: 99%

In vivo Imaging of <b><i>Sarcoptes scabiei</i></b> Infestation Using Optical Coherence Tomography

et al. 2013

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Background:Sarcoptes scabiei can be visualized with different imaging tools. Optical coherence tomography (OCT) may have the potential to describe the changes in skin morphology due to scabies infestation and visualize the parasite. Methods: Five patients from the Departments of Dermatology, Augsburg, Germany, and Roskilde, Denmark, were OCT scanned (VivoSight®; Michelson Diagnostics Ltd., UK). Mites were identified by epiluminescence and light microscopy to confirm the diagnosis. Results: OCT identified S. scabiei mites in all patients in vivo. Mites and burrows were visualized, and some detail on burrow content was provided. Conclusion: OCT can visualize S. scabiei mites in vivo, suggesting that it may be used to study the biology of the mites in vivo and provide early assessment of scabicide therapy. OCT is able to visualize structures in the skin with an 8-µm resolution. Therefore, this technology could potentially allow rapid, non-invasive, in vivo diagnosis and analysis of infestations.

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Reflectance Confocal Microscopic Features of Dermatophytes, Scabies, and <emph type="ital">Demodex</emph>

Cited by 35 publications

References 2 publications

High-Definition Optical Coherence Tomography for the in vivo Detection of Demodex Mites

High-Definition Optical Coherence Tomography for the in vivo Detection of Demodex Mites

In vivo Confocal Microscopy Features of Cutaneous Leishmaniasis

In vivo Imaging of <b><i>Sarcoptes scabiei</i></b> Infestation Using Optical Coherence Tomography

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