Reflectance confocal microscopy of tinea capitis: comparing images with results of dermoscopy and mycological exams

Veasey, John Verrinder; Meneses, Olivia Mercilene Silva; Silva, Fabiana O. da

doi:10.1111/ijd.14241

Cited by 12 publications

(8 citation statements)

References 9 publications

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“…RCM visualization of fungi in tinea capitis was shown to improve diagnosis and therapy. Tinea capitis may present as ectothrix (with Microsporum hyphae parasitizing on the outside of hair) or endothrix (with Trichophyton hyphae inside the hair shaft) [ 19 ]. Microsporum is treated with griseofulvin while trichophyton is treated with terbinafine.…”

Section: Resultsmentioning

confidence: 99%

Applications of Reflectance Confocal Microscopy in the Diagnosis of Fungal Infections: A Systematic Review

Khan

Farabi

Navarrete‐Dechent

et al. 2022

JoF

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Cutaneous and adnexal fungal infections are typically diagnosed with potassium hydroxide (KOH) skin scrapings, fungal cultures, and Periodic acid-Schiff (PAS) biopsy staining. All three current methods of fungal diagnosis require sample processing and turnover time which leads to a delay in diagnosis. Reflectance confocal microscopy (RCM) is a non-invasive, in vivo skin imaging technology that provides real-time dermatologic diagnoses. We present an updated systematic review of the applications of RCM in diagnosing fungal infections in an effort to explore the utility of RCM as an adjunct clinical tool in detecting cutaneous and adnexal fungi We systematically searched the MEDLINE (via PubMed) for studies published from January 2000 to October 2022 that described the utility of RCM in the setting of fungal infections. Of the 25 studies that met the inclusion criteria, 202 patients were included. The following information on the application of RCM in the setting of fungal infections was extracted from each study, if reported: study type, year published, number of patients included, diagnosis/diagnostic methods, and RCM description. Concordant within all included studies, fungal infections presented on RCM as bright, linear, branching, filamentous structures at the level of stratum corneum. A limitation of this review is that 11 of 25 studies were case reports (n = 1). Larger scale studies should be conducted to explore the utility of RCM in diagnosing fungal infections and to enrich the RCM descriptions of specific fungal conditions.

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Section: Resultsmentioning

confidence: 99%

Applications of Reflectance Confocal Microscopy in the Diagnosis of Fungal Infections: A Systematic Review

Khan

Farabi

Navarrete‐Dechent

et al. 2022

JoF

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“…Comparative dermatological analysis has produced important findings in infectious diseases in demonstrating that patterns of clinical and dermatoscopic parasitism repeat on histology and confocal microscopy [8][9][10]. A recent study showed that nail dermoscopy (onychoscopy) in onychomycosis reveals a statistically significant pattern of yellowed-longitudinal lines or striations (spiked or "fringe" pattern) for the diagnosis of nail fun-gus infection [11].…”

Section: Discussionmentioning

confidence: 99%

Nail Clipping with PAS Stain and Correlation with Fungi Isolated in Culture: A Valuable Exchange in the Diagnosis of Onychomycosis

Trevisan

Veasey

Werner

2022

Skin Appendage Disord

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Introduction: The etiological agents of onychomycosis are dermatophyte fungi, nondermatophyte filamentous fungi (NDFF), and yeasts. Nail clippings are an accurate tool for diagnosing onychomycosis. The objective of this study was to correlate the characteristics of fungi observed in nail clipping examinations with the results for agents isolated in culture, indicating whether the fungus found on microscopy is a dermatophyte, NDFF, or yeast. Methods: An observational descriptive study of onychomycosis nail clippings stained with PAS comparing culture results with microscopic criteria of fungi morphology, arrangement/orientation, and location of organisms in the nail and presence of neutrophils, serous lakes, and bacterial colonies was conducted. Results: Thirty-three nail clippings from 29 participants were analyzed. A statistically significant correlation (p < 0.05) was found between smooth/thin/long hyphae with a “combed” pattern and dermatophyte agents (sensitivity 89.4%, specificity 91.5%), and between irregular/thick/short anarchic distributed hyphae and nondermatophytes (sensitivity 61.5%, specificity 89.4%). No statistical association with region where fungi were in the nail or with the presence of neutrophils, serous lakes or bacteria was found. Small, rounded, yeast-like organisms correlated with Candida infection. Conclusions: Although not a substitute for culture, microscopic analysis of fungal structures offers good accuracy for differentiating agents of onychomycosis.

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“…Dermatophyten wie bei Tinea corporis oder Tinea capitis weisen Hyphen und Sporen auf, die in der KLM aufgrund ihrer hohen Reflexivität als lineare, z. T. verzweigte Strukturen nachweisbar sind [13,14,23]. Dies ist auch in .…”

Section: Methoden Liefern Morphologische Informationen Von Hautläsionenunclassified

Nichtinvasive bildgebende Diagnostik bei Hauterkrankungen im Kindesalter

2021

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Reflectance confocal microscopy of tinea capitis: comparing images with results of dermoscopy and mycological exams

Cited by 12 publications

References 9 publications

Applications of Reflectance Confocal Microscopy in the Diagnosis of Fungal Infections: A Systematic Review

Applications of Reflectance Confocal Microscopy in the Diagnosis of Fungal Infections: A Systematic Review

Nail Clipping with PAS Stain and Correlation with Fungi Isolated in Culture: A Valuable Exchange in the Diagnosis of Onychomycosis

Nichtinvasive bildgebende Diagnostik bei Hauterkrankungen im Kindesalter

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