Reflecting Microscope Objectives with Nonspherical Mirrors

Ultrasensitive optical imaging with lanthanide lumiphores

“…The trans-illumination configuration allows placement of a UV-reflecting coverglass between the sample and microscope objective, preventing the excitation of photoluminescent materials in the lenses. In principle, optics-derived background signals could be averted by other approaches such as reflective objectives 43 , 44 , darkfield microscopy 45 , and light sheet microscopy 46 . The planar illumination of light-sheet microscopy also suppresses sample autofluorescence and minimizes phototoxicity, and this method holds particular promise for time-resolved lanthanide imaging.…”

Microscopic photodensitometry and microspectrophotometry using a fixed double‐aperture optical system and an electronically controlled automatic scanning microscope stage*

“…These scans include a human cancer cell taken from an iliac crest biopsy, salivary gland chromosome preparation from Drosophilia melanogaster, and mouse skeletal muscle. The use of small apertures allows for the recording of protoplasmic features with improved fidelity (Miyata et al, 1952;Utsumi, 1959;andCaspersson et al, 1960, 1962). This point is demonstrated in the case of the striated-muscle scans showing increased reproducibility with the use of smaller equivalent apertures (Fig.…”

Chemical Microscopy

“…= 0.6 objective. Johnson (186) and others (280,292,293) also report special reflection objectives. Mellors (274) has published a discussion on reflecting microscopes.…”