Reflecting on Mechanical Functionalities in Bioreactors for Tissue Engineering Purposes

Nadhif, Muhammad Hanif; Assyarify, Hanif; Waafi, Affan Kaysa; Whulanza, Yudan

doi:10.14716/ijtech.v11i5.4314

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…The influence of UCBS and PRP coatings was examined on the PCL scaffold's mechanical properties. The mechanical properties of the scaffold are a critical factor in tissue engineering as they govern the cell's behavior (Mubarok, Elvitigala, and Sakai 2022;Mubarok, Qu, and Sakai, 2021;Nadhif et al, 2020). Herein, the mechanical properties were evaluated by measuring Young's modulus based on the stress-strain curve (Figure 2a), and the result was depicted in Figure 2b.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

The Effect of Umbilical Cord Blood Serum and Platelet-Rich Plasma Coatings on the Characteristics of Poly(?-caprolactone) Scaffolds for Skin Tissue Engineering Applications

Nurhayati,

Laksono,

Salwa

et al. 2023

IJTech

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The need for effective artificial skin as a substitute for damaged skin in chronic wound therapy is recently growing. Poly(ε-caprolactone) (PCL) has been identified as a potential material for artificial skin scaffolds due to its exceptional mechanical properties and biocompatibility. However, PCL lacks sufficient bioactivity, necessitating the introduction of bioactive molecules to scaffolds. Human umbilical cord blood serum (UCBS) and platelet-rich plasma (PRP), rich in bioactive molecules, are promising coating materials for PCL-based scaffolds. Therefore, this research aimed to investigate the effect of UCBS and PRP coatings on the mechanical properties, cytotoxicity, and cell attachment ability of PCL scaffolds. Scaffolds prepared through glutaraldehyde-mediated cross-linking of 20% (w/v) PCL followed by freeze-drying were immersed with UCBS or PRP overnight. Coating scaffolds with UCBS generated a significantly lower Young's modulus (0.20 MPa) compared to non-coated counterparts (0.27 MPa), while PRP-coated scaffolds showed no substantial change (0.24 MPa). Both UCBS and PRP coatings significantly increased (p < 0.05) the viability and attachment of primary human fibroblast cells on scaffolds, showing the potential to enhance PCL cytocompatibility for artificial skin.

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Section: Mechanical Propertiesmentioning

confidence: 99%

The Effect of Umbilical Cord Blood Serum and Platelet-Rich Plasma Coatings on the Characteristics of Poly(?-caprolactone) Scaffolds for Skin Tissue Engineering Applications

Nurhayati,

Laksono,

Salwa

et al. 2023

IJTech

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“…The MTDR could also be simulated with biaxial and multiaxial motions to determine the area that is the most affected by physiological stresses [ 106 , 111 ]. Apart from the in silico approach, the MTDR should be tested in vitro using a bioreactor, which could provide mechanical stimulations of the material [ 112 ]. The mechanical stimulations could be applied with uniaxial, biaxial, and multiaxial motions.…”

Section: Future Perspectivementioning

confidence: 99%

Anatomically and Biomechanically Relevant Monolithic Total Disc Replacement Made of 3D-Printed Thermoplastic Polyurethane

Nadhif

Ghiffary²,

Irsyad

et al. 2022

Polymers

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Various implant treatments, including total disc replacements, have been tried to treat lumbar intervertebral disc (IVD) degeneration, which is claimed to be the main contributor of lower back pain. The treatments, however, come with peripheral issues. This study proposes a novel approach that complies with the anatomical features of IVD, the so-called monolithic total disc replacement (MTDR). As the name suggests, the MTDR is a one-part device that consists of lattice and rigid structures to mimic the nucleus pulposus and annulus fibrosus, respectively. The MTDR can be made of two types of thermoplastic polyurethane (TPU 87A and TPU 95A) and fabricated using a 3D printing approach: fused filament fabrication. The MTDR design involves two configurations—the full lattice (FLC) and anatomy-based (ABC) configurations. The MTDR is evaluated in terms of its physical, mechanical, and cytotoxicity properties. The physical characterization includes the geometrical evaluations, wettability measurements, degradability tests, and swelling tests. The mechanical characterization comprises compressive tests of the materials, an analytical approach using the Voigt model of composite, and a finite element analysis. The cytotoxicity assays include the direct assay using hemocytometry and the indirect assay using a tetrazolium-based colorimetric (MTS) assay. The geometrical evaluation shows that the fabrication results are tolerable, and the two materials have good wettability and low degradation rates. The mechanical characterization shows that the ABC-MTDR has more similar mechanical properties to an IVD than the FLC-MTDR. The cytotoxicity assays prove that the materials are non-cytotoxic, allowing cells to grow on the surfaces of the materials.

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“…The 3D cell culture which is the main feature of this bioreactor has become new standard (Khafaga et al, 2022;Pichler et al, 2022). Moreover, the substrate and cell interaction are being optimized in current setting such as using the perfusion of fludic movement for benefit of cell viability (Yu et al 2022;Nadhif et al, 2020). The observation of single cell has also put important milestone in the trend of bioreactor (Czosseck et al, 2022;Clement et al 2022;Whulanza et al, 2014).…”

Section: Trial Testmentioning

confidence: 99%

Development of Auto-PIVOT: Automated Platform In Vitro for Cell Tissue Culture

Irsyad¹,

Whulanza²,

Katili³

et al. 2022

IJTech

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Bioreactors are growing in popularity among network engineers. Bioreactors are generally chambers used for cell culture processes with predetermined parameters. Pivot is a bioreactor system for cell culture that is controlled automatically and remotely using multichambers that allow inflow of fresh liquid or output for sample collection. This system facilitates gas exchange between the culture medium and ambient gas. The system provides a storage bag for fresh media and a peristaltic pump is used to move the media in a closed loop during perfusion and stirring in the cell culture chamber. The flow rates used is 2 mL/min. The system has a container for direct sample measurement to obtain pH, oxygen, and carbon dioxide parameters. The bioreactor system is also equipped with a mixing vessel which allows the addition of desired nutrients or additives to the system. The results of a computational fluid dynamics (CFD) and shear stress-based flow analysis have been conducted. The simulation results demonstrate that the applied Pivot parameters correspond to an ideal environment to hepatocyte cell viability and growth. This research is expected to increase the number of cells produced without reducing the quality of each chamber and be carried out simultaneously, automatically, and remotely controlled.

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Reflecting on Mechanical Functionalities in Bioreactors for Tissue Engineering Purposes

Cited by 6 publications

References 29 publications

The Effect of Umbilical Cord Blood Serum and Platelet-Rich Plasma Coatings on the Characteristics of Poly(?-caprolactone) Scaffolds for Skin Tissue Engineering Applications

The Effect of Umbilical Cord Blood Serum and Platelet-Rich Plasma Coatings on the Characteristics of Poly(?-caprolactone) Scaffolds for Skin Tissue Engineering Applications

Anatomically and Biomechanically Relevant Monolithic Total Disc Replacement Made of 3D-Printed Thermoplastic Polyurethane

Development of Auto-PIVOT: Automated Platform In Vitro for Cell Tissue Culture

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