Accessibility determination has become one of the key issues to interpret the relationship between urban form and travel pattern in metropolitan areas. Although the use of urban planning and design and associated accessibility influence travel makes intuitive sense, researchers have found it difficult to provide clear evidence of the influence of urban form. At the same time, it is generally recognized that land use patterns and transportation patterns are closely related to each other through accessibility change. The spatial organization of human activities creates a pattern of personal travel and goods transport, thus influences the mobility behaviour of actors such as households and firms. Conversely the availability of infrastructure makes certain locations more or less accessible. This paper identifies spatially desegregated micro-macro configurations of structural flow derived from accessibility analysis and their relationships with urban block size, road and metro-line network design, metro stations and bus stop locations, commercial land use locations distribution and station usage in Shanghai, China. Using GIS and spatial Design Network Analysis (sDNA) software to perform multi-level accessibility analysis of each link in each network, this revealed that most of the metro stations, bus stops and commercial land use are co-located on parts of the road network with the highest level of micro to macro accessibility. This indicates a 'coupling multiplier effect' between metro stations, commercial land use and multi-level multi network structural flow derived from accessibility analysis. Moreover block size is revealed as an important variable. These findings suggest the possibility of strategically appraising the impact of block size and land use planning on micro-macro accessibility change due to transportation system change and thus further the potential for systematically guiding transport oriented development planning and design place making. commercial land use and multi-level multi network structural flow derived from accessibility analysis. Moreover block size is revealed as an important variable. These findings suggest the possibility of evaluating and appraising strategically the effectiveness of block size and land use planning in relation to micro-macro accessibility change due to transportation system change and thus further the potential for systematically guiding transport oriented development planning and design place making.