Reflector of the body photophore in lanternfish is mechanistically tuned to project the biochemical emission in photocytes for counterillumination

Paitio, José; Yano, Daichi; Muneyama, Etsuhiro; Takei, Shiro; Asada, Hironori; Iwasaka, Masakazu; Oba, Yuichi

doi:10.1016/j.bbrc.2019.10.197

Cited by 13 publications

(7 citation statements)

References 24 publications

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“…Their photophore reflector is similar to those found in other luminous fishes (e.g. lanternfishes) and is comprised of cells containing guanine crystals, a reflective material also found in fish skin (Herring, 2000; Levy‐Lior et al ., 2008; Paitio et al ., 2020). Given that other fishes with photophores use coelenterazine, do the cellular components of these photophores originate from similar progenitor cells or similar cell types?…”

Section: Reviewing the Multi‐level Convergent Evolution Of Bioluminesmentioning

confidence: 99%

Multi‐level convergence of complex traits and the evolution of bioluminescence

Lau

Oakley

2020

Biological Reviews

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Evolutionary convergence provides natural opportunities to investigate how, when, and why novel traits evolve. Many convergent traits are complex, highlighting the importance of explicitly considering convergence at different levels of biological organization, or ‘multi‐level convergent evolution’. To investigate multi‐level convergent evolution, we propose a holistic and hierarchical framework that emphasizes breaking down traits into several functional modules. We begin by identifying long‐standing questions on the origins of complexity and the diverse evolutionary processes underlying phenotypic convergence to discuss how they can be addressed by examining convergent systems. We argue that bioluminescence, a complex trait that evolved dozens of times through either novel mechanisms or conserved toolkits, is particularly well suited for these studies. We present an updated estimate of at least 94 independent origins of bioluminescence across the tree of life, which we calculated by reviewing and summarizing all estimates of independent origins. Then, we use our framework to review the biology, chemistry, and evolution of bioluminescence, and for each biological level identify questions that arise from our systematic review. We focus on luminous organisms that use the shared luciferin substrates coelenterazine or vargulin to produce light because these organisms convergently evolved bioluminescent proteins that use the same luciferins to produce bioluminescence. Evolutionary convergence does not necessarily extend across biological levels, as exemplified by cases of conservation and disparity in biological functions, organs, cells, and molecules associated with bioluminescence systems. Investigating differences across bioluminescent organisms will address fundamental questions on predictability and contingency in convergent evolution. Lastly, we highlight unexplored areas of bioluminescence research and advances in sequencing and chemical techniques useful for developing bioluminescence as a model system for studying multi‐level convergent evolution.

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Section: Reviewing the Multi‐level Convergent Evolution Of Bioluminesmentioning

confidence: 99%

Multi‐level convergence of complex traits and the evolution of bioluminescence

Lau

Oakley

2020

Biological Reviews

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“…The luminescence spectra maximum (454 nm) of D. watasei luciferase from head photophores is at the same wavelength as that of D. watasei luciferase ventral photophores (Fig. 1B) [5]. We did not measure luminescence spectra of these tissues because these activities may be due to non-specific proteins other than luciferase.…”

Section: The Properties Of Native Diaphus Luciferasementioning

confidence: 98%

“…The dotted line is the wavelength of D. watasei for coelenterazine luciferase in head photophore not smoothing. The dash lined of D. watasei luciferase for coelenterazine in ventral photophore smoothing [5] Luminescence activities (blue line) with the addition of coelenterazine were measured. The activity fractions (indicated as red bars) were pooled and applied for the next purification or SDS-PAGE.…”

Section: Nanolc-ms/ms Analysismentioning

confidence: 99%

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14-3-3 proteins are luciferases candidate proteins from lanternfish Diaphus watasei

Yano

Bessho-Uehara

Paitio

et al. 2022

Photochem Photobiol Sci

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The lanternfish is a deep-sea fish with ventral-lateral and head photophores. It uses its ventral-lateral photophores to camouflage its ventral silhouette, a strategy called counterillumination. The bioluminescent reaction of lanternfish involves coelenterazine as a substrate luciferin but the enzyme catalyzing the bioluminescent reaction has not been identified. We report a candidate enzyme of luciferase from lanternfish Diaphus watasei. We purified the luciferase and performed SDS-PAGE analysis resulted in two bands corresponding to the activity, and following mass spectrometry analysis detected three 14-3-3 proteins of which functions is known to exhibit protein-protein interactions. The molecular weights and isoelectric points of the 14-3-3 proteins were almost consistent with the luciferase properties. The addition of two 14-3-3 binding compounds, R18 peptide and fusicoccin, resulted in the inhibition of the luciferase activity. However, the two 14-3-3 recombinant proteins showed very slight luminescence activity. These results suggested that the 14-3-3 proteins are candidate luciferases of D. watasei.

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“…Susunan lamella yang berkepingkeping dan sejajar menjadikan reflektor tidak kaku atau fleksibel. Sebagian besar material yang terdapat pada reflektor cumi-cumi adalah kitin atau kolagen (Wang et al, 2019;Ling et al, 2020), sedang reflektor organ cahaya ikan dibentuk dari kristal guanin (Paitio et al, 2020). Bell et al, (2013) pada reflektor organ cahaya ikan tidak berbentuk kristal, sehingga reflektor cumi-cumi lebih fleksibel dalam pengaturan cahaya.…”

Section: Lapisan Pigmen Organ Cahayaunclassified

Reconstruction of Light Organ in Squid With The Histological Method of Electron Transmission Microscope

Pringgenies

Ariyanto²

2021

J. Molusk. Indones.

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The light organ is an electronic device that can emit light. However, there are light organs in animals that can produce light naturally. Loligo duvaucelii is a species whose biolumenesence comes from fluorescent bacteria that live in symbiosis in its ink sacs. This study aims to determine in detail the construction of the squid light organ using the transmission electron microscopy (TEM) method. The results showed that this type of squid has a pair of light organs attached to the dorso-lateral ink sac. The light organ is spherical, some are found on the surface and some are embedded on the wall of the ink sac. It consists of a lens that is located on the outer surface of the ink sac, and a sac of light organs (embedded on the wall of the ink sac) with channels connecting the pocket to the mantle cavity. The wall of the sac of the light organ consists of three layers, namely the innermost layer which is multi-fold with microvilli on the cell surface and between the folds of the sac populated with bacteria, the dense layer that acts as a reflector, and the pigment layer. Cilia are observed on the surface of the duct connecting the sac with the mantle cavity. This study concluded that the construction of the squid light organ has a convex-shaped lens structure and is muscular. In the pockets of light organs, a dense population of bacteria is found. The reflector consists of many layers, and the pigment layer contains many granules.

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Reflector of the body photophore in lanternfish is mechanistically tuned to project the biochemical emission in photocytes for counterillumination

Cited by 13 publications

References 24 publications

Multi‐level convergence of complex traits and the evolution of bioluminescence

Multi‐level convergence of complex traits and the evolution of bioluminescence

14-3-3 proteins are luciferases candidate proteins from lanternfish Diaphus watasei

Reconstruction of Light Organ in Squid With The Histological Method of Electron Transmission Microscope

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