Reforming the Barrier: The Role of Formins in Wound Repair

Ahangar, Parinaz; Cowin, Allison J.

doi:10.3390/cells11182779

Cited by 4 publications

(3 citation statements)

References 124 publications

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“…In the case of CR-mediated phagocytosis, actin polymerization is associated with RhoA. This GTPase recruits and stimulates mDia formins [19]; they also activate the Arp2/3 complex.…”

Section: Recognition Of the Target Molecule By The Macrophagementioning

confidence: 99%

Macrophage: From Recognition of Foreign Agents to Late Phagocytosis

Ortiz¹,

Rodal²

2023

Phagocytosis - Main Key of Immune System

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The main line of defense that exists to eliminate foreign agents falls on phagocytic cells (neutrophils, dendritic cell, and macrophages), and it does so through phagocytosis, a complex cellular mechanism that occurs after the recognition and binding of the ligand by cellular receptors. Macrophages are part of a diverse lineage of innate immune cells. Once a macrophage receptor binds its ligand, a coordinated intracellular signaling cascade is activated to the clearance or otherwise of the foreign agent. Objects removed by macrophage phagocytosis include dead or dying host cells, cells opsonized with antibodies, and specific pathogens such as bacteria, fungi, parasites, and viruses. Currently, phagocytic macrophages have been shown to contribute to the killing of cancer cells, inflammatory bowel disease, atherosclerosis, Alzheimer’s disease, and schizophrenia. For this reason, phagocytic macrophages are important in critical participation for health and disease.

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“…In the case of CR-mediated phagocytosis, actin polymerization is associated with RhoA. This GTPase recruits and stimulates mDia formins [19]; they also activate the Arp2/3 complex.…”

Section: Recognition Of the Target Molecule By The Macrophagementioning

confidence: 99%

Macrophage: From Recognition of Foreign Agents to Late Phagocytosis

Ortiz¹,

Rodal²

2023

Phagocytosis - Main Key of Immune System

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“…Formins promote the initiation and elongation of actin filaments to participate in a variety of biological processes, such as cell signaling, organ development, microtubule stabilization, cell division, and endocytosis. [12][13][14][15] Forminmediated actin assembly is regulated by a protein family consisting of formin-binding proteins (FNBPs/FBPs). Previous studies showed that the high expression of FNBP1 is closely related to the highly aggressive characteristics of tumor cells within gastric and breast cancers.…”

Section: Introductionmentioning

confidence: 99%

FNBP4 is a Potential Biomarker Associated with Cuproptosis and Promotes Tumor Progression in Hepatocellular Carcinoma

Zheng¹,

Zhang²,

Wu³

et al. 2023

IJGM

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Background Hepatocellular carcinoma (HCC) is one of the most common malignant tumors that lacks an efficient therapeutic approach because of its elusive molecular mechanisms. This study aimed to investigate the biological function and potential mechanism of formin-binding protein 4 (FNBP4) in HCC. Methods FNBP4 expression in tissues and cells were detected by quantitative real-time PCR (qRT‒PCR), Western blot, and immunohistochemistry (IHC). The Kaplan-Meier method was used to explore the correlation between the FNBP4 expression and clinical survival. MTT, EdU incorporation, colony formation, and Transwell assays were performed to evaluate the function of FNBP4 in cell proliferation and migration in vitro. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to explore the potential mechanism of FNBP4. The prognostic risk signature and nomogram were constructed to demonstrate the prognostic value of FNBP4. Results We found that FNBP4 was upregulated in patients with HCC and associated with poor overall survival (OS). Furthermore, knockdown of FNBP4 inhibited the proliferation and migration in HCC cells. Then, we performed a KEGG pathway analysis of the coexpressed genes associated with FNBP4 and found that FNBP4 may be associated with tumor-related signaling pathways and cuproptosis. We verified that FNBP4 could cause cell cycle progression and inactivation of the hippo signaling pathway. A prognostic risk signature containing three FNBP4-related differentially expressed cuproptosis regulators (DECRs) was established and can be used as an independent risk factor to evaluate the prognosis of patients with HCC. In addition, a nomogram including a risk score and clinicopathological factors was used to predict patient survival probabilities. Conclusion FNBP4, as a potential biomarker associated with cuproptosis, promotes HCC cell proliferation and metastasis. We provide a new potential strategy for HCC treatment by targeting FNBP4.

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“…The RF-EMF is non-ionizing energy at the short-wave radiofrequency band of the electromagnetic spectrum that may be used as a non-invasive therapy delivered through a wound's dressing using small and portable devices that can be self-applied [6]. Researchers found that it may affect nitric Biosensors 2023, 13, 763 2 of 15 oxide signaling, the modulation of cytokine profiles, the expression of growth factors and the regulation of mitogen-activated protein kinase/extracellular signal-regulated kinase, resulting in the wound healing [7].…”

Section: Introductionmentioning

confidence: 99%

A Novel and Versatile Microfluidic Device for Cell Assays under Radio Frequency Exposure

et al. 2023

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Wound healing is a complex process composed of different stages, which involves extensive communication between the different cellular factors of the extracellular matrix (ECM). The radio frequency electromagnetic field (RF-EMF) has been used to accelerate the wound-healing process and it has been found to enhance cell alignment and mobility. The conventional methods for cell mobility analysis in an electromagnetic field generated by a radiation source are not advisable due to the low-precision, nonuniform distribution of the field, low efficiency of the analysis in batch and the lack of system integration for autonomous on-body operation. Here, a novel and versatile electromagnetic exposure system integrated with a microfluidic chip was fabricated to explore the EMF-induced response. A gradient electromagnetic field in a two-dimensional plane has been successfully established in the microchambers placed along the field line. In this work, by deploying our radiation experiments in vitro, we validated the on-chip monitoring of cell response to exposure. This electromagnetic field was simulated and human amniotic epithelial cells (HAECs) were cultured in different microchambers for continuous exposure to the electromagnetic field excited by a monopole RF antenna (1.8 GHz). New protrusions were generated and an obvious increase in filopodia with the increased field intensity was investigated. Meanwhile, the variation in intracellular Ca2+ concentration under the electromagnetic field was examined. The inhibitory effect of the Ca2+ circulation was further inspected to reveal the potential downstream signaling pathway in the RF-EMF-related bioassay, suggesting that cytoskeletal dynamics of cells under exposure are highly associated with the EGF receptor (EGFR)-cytoskeleton downstream signaling pathway. Finally, the field-induced cell elongation and alignment parallel to the field direction were observed. Additionally, the subsequent recovery (field withdrawal) and re-establishment (field re-exposure) were explored. These results indicated that this reliable and versatile exposure system for bioassay could achieve precise and high-throughput detection of the RF-EMF-induced cytoskeletal reorganization in vitro and evaluate the possible health risk from RF-EMF exposure.

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Reforming the Barrier: The Role of Formins in Wound Repair

Cited by 4 publications

References 124 publications

Macrophage: From Recognition of Foreign Agents to Late Phagocytosis

Macrophage: From Recognition of Foreign Agents to Late Phagocytosis

FNBP4 is a Potential Biomarker Associated with Cuproptosis and Promotes Tumor Progression in Hepatocellular Carcinoma

A Novel and Versatile Microfluidic Device for Cell Assays under Radio Frequency Exposure

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