Refractive Index Measurement of the Crystalline Lens in Vivo

Abstract

Significance This study provides a new method to measure the refractive index of crystalline lens in the human eye in vivo. Purpose Accessing the refractive index of crystalline lenses in the human eye in vivo has long been a challenge. This study aimed to measure the refractive index of a lens in vivo using an anterior segment optical coherence tomography (AS-OCT) system combined with a Scheimpflug imaging system. Method A ray-traceable Scheimpflug imaging was developed and integrated into an AS-OCT system. A theoretical study revealed that the combination of these two systems provides a unique solution for simultaneously measuring the refractive index and the thickness of the lens. The average lens refractive index along the ray pathway (Nav) and lens shape were measured for seven subjects. Results The lens Nav along the central ray varies from 1.383 to 1.419 between subjects. The lens refractive index function across the lens diameter varies from subject to subject. The lens Nav increases for accommodated eyes. The thicknesses and profiles of the lenses are also determined. Conclusions The lens refractive index varies substantially from individual eye to individual eye, not only along the central ray pathway but also for the lens refractive index function across the lens diameter. Ray-traceable Scheimpflug imaging-equipped AS-OCT is useful for testing the refractive index of lenses in the human eye in vivo.