Refreshable Nanobiosensor Based on Organosilica Encapsulation of Biorecognition Elements

Gupta, Rohit; Luan, Jingyi; Chakrabartty, Shantanu; Scheller, Erica L.; Morrissey, Jeremiah J.; Singamaneni, Srikanth

doi:10.1021/acsami.9b17506

Cited by 7 publications

(12 citation statements)

References 55 publications

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“…After functionalization, an organosiloxane polymer layer was coated on AuNR-IgG bionanoconjugates through in situ polymerization of trimethoxypropylsilane (TMPS) and (3-aminopropyl)trimethoxysilane (APTMS) in borate-buffered saline. The organosiloxane polymer protects IgG antibodies from degradation under harsh conditions. , The polymer-encapsulated AuNR-IgG bionanoconjugates solution is described as bioplasmonic ink. AuNRs were used as nanotransducers because of the high refractive index sensitivity and a tunable longitudinal LSPR wavelength (LLSPR) by changing the aspect ratio .…”

Section: Resultsmentioning

confidence: 99%

“…Plasmonic biosensors based on localized surface plasmon resonance (LSPR) can provide label-free, highly sensitive quantification of biomarkers in complex biofluids. − Recent advances in preserving the functionality of plasmonic biosensors have improved the stability of these biosensors and their reliable performance for PON diagnostics. − For example, metal-organic framework coating can retain the biorecognition capability of antibodies immobilized on the nanostructure surface in plasmonic biosensors after exposure to elevated temperatures. , However, this approach requires protective coating removal before introducing the sample to the sensors. Alternatively, organosiloxane polymer-encapsulated antibodies render plasmonic biosensors improved thermal, mechanical, chemical, and biological stability. , However, these sensors rely on rigid two-dimensional (2D) substrates and cannot handle small-volume biofluids for biomarker quantification. A plasmonic biosensing platform with stable sensing components and the capability of handling small-volume biofluids is needed for reliable and user-friendly diagnostics at the PON.…”

Section: Introductionmentioning

confidence: 99%

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Printed Ultrastable Bioplasmonic Microarrays for Point-of-Need Biosensing

Guo

Yin

Namkoong

et al. 2022

ACS Appl. Mater. Interfaces

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Paper-based point-of-need (PON) biosensors are attractive for various applications, including food safety, agriculture, disease diagnosis, and drug screening, owing to their low cost and ease of use. However, existing paper-based biosensors mainly rely on biolabels, colorimetric reagents, and biorecognition elements and exhibit limited stability under harsh environments. Here, we report a label-free paper-based biosensor composed of bioplasmonic microarrays for sensitive detection and quantification of protein targets in small volumes of biofluids. Bioplasmonic microarrays were printed using an ultrastable bioplasmonic ink, rendering the PON sensors excellent thermal, chemical, and biological stability for their reliable performance in resourcelimited settings. We fabricated silicone hydrophobic barriers and bioplasmonic microarrays with direct writing and droplet jetting approaches on a three-dimensional (3D) nanoporous paper. Direct writing hydrophobic barriers can define hydrophilic channels less than 100 μm wide. High-resolution patterning of hydrophilic test domains enables the handling and analysis of small fluid volumes. We show that the plasmonic sensors based on a vertical flow assay provide similar sensitivity and low limit of detection with a 60 μL sample volume compared to those with 500 μL samples based on an immersion approach and can shorten assay time from 90 to 20 min.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Printed Ultrastable Bioplasmonic Microarrays for Point-of-Need Biosensing

Guo

Yin

Namkoong

et al. 2022

ACS Appl. Mater. Interfaces

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“…SPR method has the advantages of real-time, fast, and high sensitivity for NGAL detection compared with the traditional ELSA method 130 - 132 . Recently, Gupta et al developed reusable SPR biosensor based on the NGAL antibody 133 ( Figure 10 A ). NGAL antibody was modified on gold nanorods, and then adopted silica shell to maintain the stability of the NGAL antibody by copolymerization of (3-aminopropyl)-trimethoxysilane and trimethoxy (propyl) silane around the NGAL antibody.…”

Section: Ngalmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Emerging early diagnostic methods for acute kidney injury

Xiao¹,

Huang²,

Yang³

et al. 2022

Theranostics

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Many factors such as trauma and COVID-19 cause acute kidney injury (AKI). Late AKI have a very high incidence and mortality rate. Early diagnosis of AKI provides a critical therapeutic time window for AKI treatment to prevent progression to chronic renal failure. However, the current clinical detection based on creatinine and urine output isn't effective in diagnosing early AKI. In recent years, the early diagnosis of AKI has made great progress with the advancement of information technology, nanotechnology, and biomedicine. These emerging methods are mainly divided into two aspects: First, predicting AKI through models construct by machine learning; Second, early diagnosis of AKI through detection of newly-discovered early biomarkers. Currently, these methods have shown great potential and become an attractive tool for the early diagnosis of AKI. Therefore, it is very important to discuss and summarize these methods for the early diagnosis of AKI. In this review, we first systematically summarize the application of machine learning in AKI prediction algorithms and specific scenarios. In addition, we introduce the key role of early biomarkers in the progress of AKI, and then comprehensively summarize the application of emerging detection technologies for early AKI. Finally, we discuss current challenges and prospects of machine learning and biomarker detection. The review is expected to provide new insights for early diagnosis of AKI, and provided important inspiration for the design of early diagnosis of other major diseases.

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“…Plasmonic-uors consists of plasmonically active core, gold nanorod synthesized by seed-mediated method, 56 a polymer spacer layer, uorophores and universal biorecognition element (biotin). Plasmonicuors were synthesized following the similar procedure described in our previous study.…”

Section: Synthesis Of Plasmonic-uorsmentioning

confidence: 99%

Plasmon-enhanced Quantitative Lateral Flow Assay for Femtomolar Detection of SARS-CoV-2 Antibodies and Antigens

Gupta

Wang

et al. 2022

Preprint

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Lateral flow assays (LFAs) are the cornerstone of point-of-care diagnostics. Although rapid and inexpensive, they are 1000-fold less sensitive than laboratory-based tests and cannot be used for definitive negative diagnosis. Here, we overcome this fundamental limitation by employing plasmonically-enhanced nanoscale colorimetric and fluorescent labels. Plasmonic LFAs (p-LFAs) enabled ultrasensitive detection and quantification of low abundance analytes, without compromising the direct visual detection of conventional LFAs. Dynamic ranges and limits of detection were up to 100-fold superior to “gold standard” ELISA (enzyme-linked immunosorbent assay). p-LFAs had sample-to-answer time of 20 min, compared to 4 hours for ELISA, while achieving over 95% analytical sensitivity and 100% analytical specificity for antibodies and antigens of SARS-CoV-2 in human specimens. We also demonstrate that the p-LFAs enable quantitative detection of the target analytes in a standard-free manner. p-LFAs offer potential as a broadly adaptable point-of-care diagnostic platform that outperforms standard laboratory tests in sensitivity, speed, dynamic range, ease of use, and cost.

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Refreshable Nanobiosensor Based on Organosilica Encapsulation of Biorecognition Elements

Cited by 7 publications

References 55 publications

Printed Ultrastable Bioplasmonic Microarrays for Point-of-Need Biosensing

Printed Ultrastable Bioplasmonic Microarrays for Point-of-Need Biosensing

Emerging early diagnostic methods for acute kidney injury

Plasmon-enhanced Quantitative Lateral Flow Assay for Femtomolar Detection of SARS-CoV-2 Antibodies and Antigens

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