Regeneração in vitro de melão, cv. 'Gaúcho'

Abstract

(0.5; 0.9; 1.5 and 2.0mg L -1

Regeneration of cotyledonary nodes from the recalcitrant melon cultivar Gacho

“…Furthermore, the percentage of regenerating explants ranged from 60 to 100% (average for all treatments, 94%) of the cotyledonary nodes inoculated in the regeneration media, determining a high efficiency in the final production process of in vitro plants and hence in acclimatization. These results are much higher than those obtained by Pinho et al (2010) who used the same cultivar and another explant (cotyledons from newly germinated seeds) and achieved only a regeneration rate of 29.66%, even when the seeds were germinated on MS medium containing BAP. The histological analyses confirmed the development of shoot apical meristems and leaf primordia from cotyledonary nodes (Figure 4), and the adventitious shoots were more developed when formed in germination media with a low BAP concentration (Figure 2).…”

“…The use of BAP during seed germination determined an impairment of growth and development of seedlings and roots, as observed in Arabidopsis thaliana (Cary et al, 1995;Muraro et al, 2013). The positive effect of BAP on the regeneration of shoots has been mostly reported in melons (Ntui et al, 2009;Pinho et al, 2010) and other species such as watermelon (Chaturvedi and Bhatnagar, 2001;Krug et al, 2005), and cucumber (Selvaraj et al, 2007). However, few studies have been performed on the positive effect of pre-treatments before the regeneration stage with growth regulators.…”

“…In Brazil, mostly in the southeast, cultivated melons belong to the saccharinus variety and have medium-to big-sized fruits that are round or oblong with white or yellow and very sweet flesh (Nunez-Palenius et al, 2008). The cultivar 'Gaúcho' is cultivated extensively, even though it produces perishable fruits with a shelf life of 4 to 6 days after harvest (Pinho et al, 2010). Traditional hybridization techniques have been used for melon breeding; however, there is an increasing demand for new cultivars with a wide variety of characteristics, such as high levels of disease resistance and enhanced flavor and sweetness (Chovelon et al, 2011).…”

“…Although several methods have been reported in the literature for plant regeneration with different explants (Chaturvedi and Bhatnagar, 2001;Curuk et al, 2003;Akasaka-Kennedy et al, 2004;Comlekcioglu et al, 2009), the efficiency of the techniques for melons has been usually low and highly dependent on the genotype (Galperin et al, 2003;Nunez-Palenius et al, 2008;Chovelon et al, 2011) and the explant source (Guis et al, 2000;Pinho et al, 2010). Besides, it is very common that induced buds do not develop into normal shoots or plantlets (Stipp et al, 2001).…”

In vitro shoot regeneration of boldo from leaf explants

“…However, the first requirement for successful application of biotechnology is to have high-frequency shoot regeneration from cultured cells and tissues. It is well known that the regeneration process from differentiated tissues differs among plant species and tissue due to several internal and external factors including the type and hormonal concentration in the explants (PINHO et al, 2010). The auxin: cytokin ratio is one important factor affecting in vitro development and leaves are common explants in plant regeneration studies (GOLEGAONKAR & KANTHARAJAH, 2006).…”