Regeneração in vitro de Parapiptadenia rigida

Kielse, Paula; Franco, Elci Terezinha Henz; Paranhos, Juçara Terezinha; Lima, Ana Paula Santos de

doi:10.1590/s0103-84782009005000046

Cited by 16 publications

(18 citation statements)

References 6 publications

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“…Embora nem sempre sejam necessárias no meio de multiplicação, as auxinas são utilizadas com o intuito de estimular o crescimento da parte aérea por meio da expansão e do alongamento celular e induzir raízes adventícias. As citocininas são indispensáveis para a quebra da dominância apical e indução de proliferação de gemas axilares (GRATTAPAGLIA & MACHADO, 1998;KIELSE et al, 2009).…”

Section: Erythrina Velutinaunclassified

Propagação in vitro de Erythrina velutina

2010

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Section: Erythrina Velutinaunclassified

Propagação in vitro de Erythrina velutina

2010

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“…(CHERUVATHUR; ABRAHAM; THOMAS, 2013) and Parapiptadenia rigida (Benth.) Brenan (KIELSE et al, 2009), both woody, the concentration of 4.92 µM IBA provided the highest rooting frequency.…”

Section: Resultsmentioning

confidence: 93%

Indirect in vitro organogenesis of Hancornia speciosa Gomes

Prudente¹,

Paiva²,

Nery³

et al. 2016

Biosci. J.

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ABSTRACT:Hancornia speciosa Gomes is a fruit species belonging to the Apocynaceae family and holds social, cultural and economic potential mostly due to its use in composition of many food industry products and the consumption its fruits in natura. Several aspects regarding their propagation need further studies, since the species is undergoing a continuous process of domestication. The objective was to obtain an in vitro protocol for indirect organogenesis and rooting with subsequent acclimatization of H. speciosa plants. To obtain indirect organogenesis, internodal segments were inoculated in WPM culture medium gelled with 7 g L -1 agar, added with 30 g L -1 sucrose, 0.4 g L -1 PVP, and supplied with different concentrations of 2,4-D (0.0; 2.46; 7.38; 12.30; and 17.22 µM), BAP (0.0; 4.92; 9.84; 14.76; and 19.68 µM), and TDZ (0.0; 4.92; 9.84; 14.76; and 19.68 µM). For the in vitro rooting, shootings with approximately 6.0 cm diameter length kept for 15 days in WPM medium with no plant growth regulator and, afterwards, subjected to treatments with different auxins (Control; 9.84 µM IBA; 9.84 µM NAA; and 9.84 µM IAA) as well as the combination among them, in order to verify their effects on percentage of rooting (%), number of roots, and average length of the largest root (cm). The formation of calluses was observed in all explants subjected to the concentrations of the regulators tested. The highest shooting regeneration occurred with 7.38 µM 2,4-D (73%). The highest percentage of shoot rooting (80%) and roots with the largest length (1.3 cm) were found in the culture medium with the combination of 4.92 µM NAA and 4.92 µM IBA. The in vitro regeneration of H. speciosa is feasible. The acclimatization of rooted shoots in Trospstrato® was accomplished with successful and 100% survival of plant material was observed during this stage.

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“…Para o explante segmento cotiledonar, verifi couse modelo matemático linear decrescente, sendo a melhor competência do explante (96,0%) observada na ausência do BAP (Figura 1C). Esses resultados diferem daqueles obtidos por KIELSE et al (2009), uma vez que os autores obtiveram 98,75% de resposta do segmento cotiledonar cultivado em meio de cultura suplementado com BAP.…”

Section: Resultsunclassified

“…Estes resultados são semelhantes aos encontrados para outras espécies lenhosas como angico (Parapiptadenia rigida) (KIELSE et al, 2009) e barbatimão (Stryphnodendren adstringens) (NICIOLI et al, 2008), em que taxas de aclimatização abaixo de 50% também foram relatadas.…”

Section: Resultsunclassified