1980
DOI: 10.1111/j.1399-3054.1980.tb03243.x
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Regenerated cell wall of carrot protoplasts isolated from suspension‐cultured cells

Abstract: Protoplasts of Daucus carota L. cultured in a synthetic liquid medium resumed cell division after about 4 days of cultivation. During this lag period, nucleic acid and protein showed only slight increases but the protoplasts commenced cell‐wall regeneration soon after the removal of lytic enzymes. The originally spherical protoplasts became ellipsoidal before they underwent division. Radioactive glucose and myo‐inositol were readily utilized by the protoplasts. Most of the radioactivity, however, appeared in e… Show more

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Cited by 27 publications
(10 citation statements)
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“…Regenerated cell walls of protoplasts are usually much different in composition from the cell walls of the original tissue and one of the major differences is a lower cellulose content [2,7,20,34]. The reasons for this are not known; however, wall regeneration by protoplasts does not reflect the normal synthesis of cell wall.…”
Section: Permethylation Analysis Of Cell Wall Glucansmentioning
confidence: 92%
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“…Regenerated cell walls of protoplasts are usually much different in composition from the cell walls of the original tissue and one of the major differences is a lower cellulose content [2,7,20,34]. The reasons for this are not known; however, wall regeneration by protoplasts does not reflect the normal synthesis of cell wall.…”
Section: Permethylation Analysis Of Cell Wall Glucansmentioning
confidence: 92%
“…In an intact plant, a process that is analogus to cell wall regeneration by protoplasts is the formation of the phragmoplast which forms the cell plate. A similar process in which a wall is formed de novo is also encountered in cellular response to injury [9] and many protoplast systems regenerate cell walls containing increased levels of/3.1,3-1inked glucans similar to callose [2,7,20,28,34], or in the case of tomato fruit protoplasts, the wound response is the production of subrin [31 ].…”
Section: Permethylation Analysis Of Cell Wall Glucansmentioning
confidence: 99%
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“…W hen the glycosidase activities are com pared, agalactosidase activity was seen to be about ten times higher than any of the other activities. This high level of a-galactosidase activity may be characteristic of Catharanthus cells, because lower level of activity of this enzyme are usually found in other plant sources [7,9,16].…”
Section: Resultsmentioning
confidence: 99%
“…We reported some dynamic aspects of cell wall metabolism during the cell cycle including changes in cell wall constituents [1], in synthetic activities [2], in UDP-sugar levels [3,4], and in glucan synthase activities [5] in a synchro nous culture system of Catharanthus cells [6 ], Relationships between cell elongation and wallbound glycosidases have been suggested by many w orkers (e.g. [7][8][9][10][11][12]). Indeed wall-bound glycosid ases are responsible for the hydrolysis of the structur al polysaccharides of plant cell walls.…”
Section: Introductionmentioning
confidence: 99%