Regenerating Critical Size Rat Segmental Bone Defects with a Self‐Healing Hybrid Nanocomposite Hydrogel: Effect of Bone Condition and BMP‐2 Incorporation

Houdt, Claire I. A. van; Koolen, M.K.E.; López-Pérez, Paula M.; Ulrich, Dietmar; Jansen, John A.; Leeuwenburgh, Sander C.G.; Weinans, Harrie; Beucken, Jeroen J.J.P. van den

doi:10.1002/mabi.202100088

Cited by 19 publications

(17 citation statements)

References 45 publications

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“…Severe trauma, bone tumors, congenital malformation, or extensive infection of bone tissue can be the causes of critical-size bone defects. A critical-size bone defect is defined as an orthotropic intraosseous wound that does not self-heal without additional surgical intervention ( Van Houdt et al, 2021 ). The repair of critical-size segmental bone defects is still a great challenge in clinic.…”

Section: Resultsmentioning

confidence: 99%

Engineering Multifunctional Hydrogel With Osteogenic Capacity for Critical-Size Segmental Bone Defect Repair

Zheng

Zhong

Cheng

et al. 2022

Front. Bioeng. Biotechnol.

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Treating critical-size segmental bone defects is an arduous challenge in clinical work. Preparation of bone graft substitutes with notable osteoinductive properties is a feasible strategy for critical-size bone defects. Herein, a biocompatible hydrogel was designed by dynamic supramolecular assembly of polyvinyl alcohol (PVA), sodium tetraborate (Na2B4O7), and tetraethyl orthosilicate (TEOS). The characteristics of the supramolecular hydrogel were evaluated by rheological analysis, swelling ratio, degradation experiments, and scanning electron microscopy (SEM). In in vitro experiments, this TEOS-hydrogel had self-healing property, low swelling rate, degradability, good biocompatibility, and induced osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by upregulating the expression of Runx-2, Col-1, OCN, and osteopontin (OPN). In segmental bone defect rabbit models, the TEOS-containing hydrogel accelerated bone regeneration, thus restoring the continuity of bone and recanalization of the medullary cavity. The abovementioned results demonstrated that this TEOS-hydrogel has the potential to realize bone healing in critical-size segmental bone defects.

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Section: Resultsmentioning

confidence: 99%

Engineering Multifunctional Hydrogel With Osteogenic Capacity for Critical-Size Segmental Bone Defect Repair

Zheng

Zhong

Cheng

et al. 2022

Front. Bioeng. Biotechnol.

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“…Because only approximately 20% of the newly formed tissue was mineralized, we intended to enhance the osteogenic potential of the ADSC/HUVEC coculture system with the application of growth factors. Vascular endothelial growth factor A isoform 165 (VEGFA165) and bone morphogenetic protein 2 (BMP2) are well known growth factors associated with bone formation and angiogenesis [17,[21][22][23]. Based on previous work and in order to translate our findings into the AV loop model, we used a concentration of 60 µg/mL BMP2 and 100 ng/mL VEGFA165 for our study [21,24,25].…”

Section: Discussionmentioning

confidence: 99%

The Adipose-Derived Stem Cell and Endothelial Cell Coculture System—Role of Growth Factors?

Steiner

Mutschall

Winkler

et al. 2021

Cells

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Adequate vascularization is a fundamental prerequisite for bone regeneration, formation and tissue engineering applications. Endothelialization of scaffold materials is a promising strategy to support neovascularization and bone tissue formation. Besides oxygen and nutrition supply, the endothelial network plays an important role concerning osteogenic differentiation of osteoprogenitor cells and consecutive bone formation. In this study we aimed to enhance the growth stimulating, proangiogenic and osteogenic features of the ADSC and HUVEC coculture system by means of VEGFA165 and BMP2 application. We were able to show that sprouting phenomena and osteogenic differentiation were enhanced in the ADSC/HUVEC coculture. Furthermore, apoptosis was unidirectionally decreased in HUVECs, but these effects were not further enhanced upon VEGFA165 or BMP2 application. In summary, the ADSC/HUVEC coculture system per se is a powerful tool for bone tissue engineering applications.

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“…The ions that are precipitated from the scaffold material itself [ 28 ], and the scaffold as a delivery carrier, transporting growth factors or drugs, can induce stem cell differentiation. Bone morphogenetic proteins and vascular endothelial growth factor have also been found to promote bone induction in scaffolds [ 29 , 30 ].…”

Section: Advances and Challenges Of Bone Defect Treatmentmentioning

confidence: 99%

Bone Tissue Engineering in the Treatment of Bone Defects

et al. 2022

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Bones play an important role in maintaining exercise and protecting organs. Bone defect, as a common orthopedic disease in clinics, can cause tremendous damage with long treatment cycles. Therefore, the treatment of bone defect remains as one of the main challenges in clinical practice. Today, with increased incidence of bone disease in the aging population, demand for bone repair material is high. At present, the method of clinical treatment for bone defects including non-invasive therapy and invasive therapy. Surgical treatment is the most effective way to treat bone defects, such as using bone grafts, Masquelet technique, Ilizarov technique etc. In recent years, the rapid development of tissue engineering technology provides a new treatment strategy for bone repair. This review paper introduces the current situation and challenges of clinical treatment of bone defect repair in detail. The advantages and disadvantages of bone tissue engineering scaffolds are comprehensively discussed from the aspect of material, preparation technology, and function of bone tissue engineering scaffolds. This paper also summarizes the 3D printing technology based on computer technology, aiming at designing personalized artificial scaffolds that can accurately fit bone defects.

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Regenerating Critical Size Rat Segmental Bone Defects with a Self‐Healing Hybrid Nanocomposite Hydrogel: Effect of Bone Condition and BMP‐2 Incorporation

Cited by 19 publications

References 45 publications

Engineering Multifunctional Hydrogel With Osteogenic Capacity for Critical-Size Segmental Bone Defect Repair

Engineering Multifunctional Hydrogel With Osteogenic Capacity for Critical-Size Segmental Bone Defect Repair

The Adipose-Derived Stem Cell and Endothelial Cell Coculture System—Role of Growth Factors?

Bone Tissue Engineering in the Treatment of Bone Defects

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