Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells

Ono, Hisayo; Obana, Aya; Usami, Yu; Sakai, Manabu; Nohara, Kanji; Egusa, Hiroshi; Sakai, Takayoshi

doi:10.1155/2015/293570

Cited by 46 publications

(38 citation statements)

References 35 publications

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“…• In mice model, histological analysis of the produced teratoma, following transplantation of iPS cell showed the presence of glandular tissues similar to both the sub-mandibular salivary gland (SMG) and the sublingual salivary gland (SLG) (22). Though iPS cells demonstrate the potential ability to regenerate SMG and SLG cells; only limited tissues differentiated was observed.…”

Section: Applications Ips Cells In Dentistry (Fig 1)mentioning

confidence: 99%

“…Though iPS cells demonstrate the potential ability to regenerate SMG and SLG cells; only limited tissues differentiated was observed. Regenerated salivary glands from iPS cell showed acinar-like structures similar to embryonic salivary glands with water channel protein in the lumen of the acinar-like structures, indicating their ability to secrete saliva (22). Also salivary glands produced from iPS cells had more number of small acinar-like structures than the salivary glands differentiated from embryonic salivary gland cells.…”

Section: Applications Ips Cells In Dentistry (Fig 1)mentioning

confidence: 99%

“…Studies have shown the usage of iPS cells, in mouse model, for differentiation into ameloblasts (20) and odontogenic mesenchymal cells (21). Also iPS cell has the pluripotency for differentiation into all three primary germ layers (1, 22). Thus iPS cells generated from discarded oral tissues can be used for autologous cell-based oral tissues regeneration (periodontal regeneration), custom modulation of oral diseases for individual patients, and to design tailor-made diagnostic tools.…”

Section: Introductionmentioning

confidence: 99%

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Induced Pluripotent Stem (iPS) Cells in Dentistry: A Review

Malhotra

2016

IJSC

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iPS cells are derived from somatic cells via transduction and expression of selective transcription factors. Both viral-integrating (like retroviral) and non-integrating (like, mRNA or protein-based) techniques are available for the production of iPS cells. In the field of dentistry, iPS cells have been derived from stem cells of apical papilla, dental pulp stem cells, and stem cells from exfoliated deciduous teeth, gingival and periodontal ligament fibroblasts, and buccal mucosa fibroblasts. iPS cells have the potential to differentiate into all derivatives of the 3 primary germ layers i.e. ectoderm, endoderm, and mesoderm. They are autogeneically accessible, and can produce patient-specific or disease-specific cell lines without the issue of ethical controversy. They have been successfully tested to produce mesenchymal stem cells-like cells, neural crest-like cells, ameloblasts-like cells, odontoblasts-like cells, and osteoprogenitor cells. These cells can aid in regeneration of periodontal ligament, alveolar bone, cementum, dentin-pulp complex, as well as possible Biotooth formation. However certain key issues like, epigenetic memory of iPS cells, viral-transduction, tumorgenesis and teratoma formation need to be overcome, before they can be successfully used in clinical practice. The article discusses the sources, pros and cons, and current applications of iPS cells in dentistry with an emphasis on encountered challenges and their solutions.

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Section: Applications Ips Cells In Dentistry (Fig 1)mentioning

confidence: 99%

Section: Applications Ips Cells In Dentistry (Fig 1)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Induced Pluripotent Stem (iPS) Cells in Dentistry: A Review

Malhotra

2016

IJSC

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“…Ono H et al [88] attempt to regenerate salivary glands using induced pluripotent stem (iPS) cells. Glandular tissues that were similar to the adult submandi- …”

Section: Animal Studies Salivary Gland Regenerationmentioning

confidence: 99%

The Current Status of Stem Cell Regeneration in Intra Oral Applications—A Systematic Review

Kanwal¹,

D’souza²,

Muthiah³

et al. 2017

OJST

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Aim: 1) To review the literature of various applications of stem cell regeneration in dentistry from 2010 to 2016. 2) To review these studies and to summarize the current status of stem cell regeneration in intra oral applications.3) To present the available successful data with regard to stem cell regeneration in dentistry and to highlight the future trends. Materials and Methods: Search Protocol: A systematic search was made in the PubMed database with the key words gingiva, papillary reconstruction, periodontal ligament, dental pulp, salivary gland, enamel re-mineralization, dentin, cementum, bone, whole tooth, cleft palate, regeneration and stem cells. All articles from 2010 to 2016, relevant to the topic were included. After the search a total of 1826 articles were obtained which were screened and categorized by three independent reviewers as review papers, in vitro, animal and human studies, pertaining to stem cell regeneration in intra oral applications. On the basis of the extracted data and outcome measures, conclusions were drawn. Results: Maximum number of animal studies has been done to regenerate periodontal tissues, bone, dentin and pulp tissues. Few human studies have been done till date. Although clinically, researchers have been able to regenerate periodontal-like tissue, bone and pulp-like tissue, they still haven't been able to regenerate tissues that completely resemble tissues in their natural form. Conclusion: The future of stem cell therapy in dental applications looks promising. The predictability and efficacy of outcomes, as well as safety concerns of stem cell therapy is yet to be fully established. Ongoing research and development of newer scaffolds, understanding various signaling molecules and their cues, understanding gene expression and proteomics of stem cells are the future directions that will take us a step forward to achieving successful regeneration.

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“…These reports include several types of stem cells such as bone marrow- (BM-) derived cells [63, 64], BM-derived mesenchymal stem cells (MSCs) [14, 52], human adipose-derived MSCs [53, 54], SG-derived MSC-like cells [55], amniotic cells [56, 57], embryonic stem cells (ESC) [58], and induced-pluripotent stem cells (iPSC) [59]. …”

Section: Nonsalivary Gland Cells and Their Secretomementioning

confidence: 99%

Three‐Dimensional Bioprinting Nanotechnologies towards Clinical Application of Stem Cells and Their Secretome in Salivary Gland Regeneration

Ferreira

Rungarunlert

Urkasemsin

et al. 2016

Stem Cells International

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Salivary gland (SG) functional damage and severe dry mouth (or xerostomia) are commonly observed in a wide range of medical conditions from autoimmune to metabolic disorders as well as after radiotherapy to treat specific head and neck cancers. No effective therapy has been developed to completely restore the SG functional damage on the long-term and reverse the poor quality of life of xerostomia patients. Cell- and secretome-based strategies are currently being tested in vitro and in vivo for the repair and/or regeneration of the damaged SG using (1) epithelial SG stem/progenitor cells from salispheres or explant cultures as well as (2) nonepithelial stem cell types and/or their bioactive secretome. These strategies will be the focus of our review. Herein, innovative 3D bioprinting nanotechnologies for the generation of organotypic cultures and SG organoids/mini-glands will also be discussed. These bioprinting technologies will allow researchers to analyze the secretome components and extracellular matrix production, as well as their biofunctional effects in 3D mini-glands ex vivo. Improving our understanding of the SG secretome is critical to develop effective secretome-based therapies towards the regeneration and/or repair of all SG compartments for proper restoration of saliva secretion and flow into the oral cavity.

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Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells

Cited by 46 publications

References 35 publications

Induced Pluripotent Stem (iPS) Cells in Dentistry: A Review

Induced Pluripotent Stem (iPS) Cells in Dentistry: A Review

The Current Status of Stem Cell Regeneration in Intra Oral Applications—A Systematic Review

Three‐Dimensional Bioprinting Nanotechnologies towards Clinical Application of Stem Cells and Their Secretome in Salivary Gland Regeneration

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