Regeneration and Agrobacterium-mediated genetic transformation of Terminalia bellerica Roxb.: a multipurpose tree species

Dangi, Bhawna; Kachhwaha, Sumita; Kothari, S. L.

doi:10.1007/s11627-012-9436-1

Cited by 9 publications

(12 citation statements)

References 37 publications

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“…At higher concentration of IBA the percentage of rooting decreased and callus formation occurred at basal cut end as also reported by Goyal et al (2012). A low survival frequency was observed when rooted plants were directly transferred from agar containing medium to soil (Dangi et al 2012). Yu et al (2000) suggested that aeration was an important factor in the formation of adventitious roots.…”

Section: Resultssupporting

confidence: 56%

“…1g), thus, confirming true-to-type nature of regenerated plants. RAPD has been widely used in genetic variation studies in tissue cultured derived plantlets as has been also reported earlier in various plants (Dangi et al 2012, Goyal et al 2012.…”

Section: Resultsmentioning

confidence: 85%

“…A maximum of 13 shoots were formed on medium with 0.55 CoCl 2 μM when nodal segments were used as an explant as compared to 9 shoots on medium with the basal concentration of cobalt (Figs 2a, 3). Increased CoCl 2 has been shown to greatly improve regeneration in other plant species also (Dangi et al 2012). Co 2+ inhibits ethylene production by blocking the conversion of l-aminocyclopropane-1-carboxylic acid to ethylene (Yang and Hoffman 1984).…”

Section: Resultsmentioning

confidence: 99%

“…Co 2+ inhibits ethylene production by blocking the conversion of l-aminocyclopropane-1-carboxylic acid to ethylene (Yang and Hoffman 1984). Thus, the enhancement of shoot regeneration with increased Co 2+ is hypothesized to be due to a decrease in the level of ethylene (Dangi et al 2012). Zinc is essential for protein synthesis, IAA synthesis and nitrogen metabolism but it is toxic at high levels and produces adverse effects on plant growth and development (Wang et al 2009).…”

Section: Resultsmentioning

confidence: 99%

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Nutrient optimization for improved in vitro plant regeneration in Eclipta alba (L.) Hassk. and assessment of genetic fidelity using RAPD analysis

Bardar

Kaul

Kachhwaha

et al. 2015

Plant Tissue Cult. & Biotech.

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This study highlights the effect of different inorganic micronutrients like copper, cobalt, molybdenum, zinc, boron, iodine, iron and manganese in accelerating and amplifying in vitro shoot bud induction and proliferation of a medicinally important plant, Eclipta alba (L.) Hassk. Direct shoot bud induction was observed on MS fortified with Kn (2 mg/l). However, maximum number of shoots was achieved when GA3, 0.5 mg/l was added to induction medium along with 1μM copper sulphate (ten times the normal MS level). Optimization of nutrient level in the basal medium promoted maximum regeneration response from both shoot tips and nodal explants. Elongated shoots were rooted in MS supplemented with IBA, 1.0 mg/l. Healthy, green plantlets with well developed roots, flowered normally in the field. Genetic stability of micropropagated plantlets was evaluated using RAPD markers. The amplification products were monomorphic in micropropagated plantlets and similar to those of mother plant revealing the genetic uniformity of plantlets. The regeneration protocol is highly efficient and reproducible so would be useful for mass multiplication, ex situ conservation and genetic transformation of E. alba (L.) Hassk.

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Section: Resultssupporting

confidence: 56%

Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Nutrient optimization for improved in vitro plant regeneration in Eclipta alba (L.) Hassk. and assessment of genetic fidelity using RAPD analysis

Bardar

Kaul

Kachhwaha

et al. 2015

Plant Tissue Cult. & Biotech.

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“…Tissue culture has proved to be a promising technique for conservation and large-scale multiplication of several woody species . Earlier reports on micropropagtion of T. bellerica used juvenile cotyledonary node as explant (Ramesh et al 2005;Rathore et al 2008;Dangi et al 2012b). Phulwaria et al (2012) reported a protocol by taking explants from 10 year old plants.…”

Section: Introductionmentioning

confidence: 99%

Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

Dangi

Khurana-Kaul

Kothari

et al. 2014

Physiol Mol Biol Plants

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The present study reports an efficient in vitro micropropagation protocol for a medicinally important tree, Terminalia bellerica Roxb. from nodal segments of a 30 years old tree. Nodal segments taken from the mature tree in MarchApril and cultured on half strength MS medium gave the best shoot bud proliferation response. )] in the culture medium aided to minimize browning and improve explant survival during shoot bud induction. Highest multiplication of shoots was achieved on medium supplemented with 6-benzyladenine (BA, 8.8 μM) and α-naphthalene acetic acid (NAA, 2.6 μM) in addition to antioxidants. Shoot elongation was obtained on MS medium containing BA (4.4 μM)+phloroglucinol (PG, 3.9 μM). Elongated shoots were transferred to half strength MS medium containing indole-3-butyric acid (IBA, 2.5 μM) for root development. The acclimatization of plantlets was carried out under greenhouse conditions. The genetic fidelity of the regenerated plants was checked using inter simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) analysis. Comparison of the bands among the regenerants and mother plant confirmed true-to-type clonal plants.

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Plant Tissue Culture: Applications in Plant Improvement and Conservation

Shahzad

Parveen

Sharma

et al. 2017

Plant Biotechnology: Principles and Applications

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Regeneration and Agrobacterium-mediated genetic transformation of Terminalia bellerica Roxb.: a multipurpose tree species

Cited by 9 publications

References 37 publications

Nutrient optimization for improved in vitro plant regeneration in Eclipta alba (L.) Hassk. and assessment of genetic fidelity using RAPD analysis

Nutrient optimization for improved in vitro plant regeneration in Eclipta alba (L.) Hassk. and assessment of genetic fidelity using RAPD analysis

Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

Plant Tissue Culture: Applications in Plant Improvement and Conservation

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