Regeneration and genetic engineering of a tropical tree, Azadirachta excelsa

Morimoto, Masayuki; Nakamura, Kentaro; Sano, Hiroshi

doi:10.5511/plantbiotechnology.23.123

Cited by 6 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Clonal forestry (CF) has enabled rapid genetic gain, thus opening the possibility for domestication and massive proliferation of elite individuals within a relatively short time (Campbell et al 2003;Merkle and Nairn 2005). CF has been used in breeding programs of the most important tropical forest genera, such as Eucalyptus (Pinto et al 2002;Ruiz et al 2005), Tectona (Hausen and Pal 2003), Cedrela (Nunes et al 2002;Peña-Ramírez et al 2010), Azadirachta (Morimoto et al 2006), Acacia (Vengadesan et al 2002), and Gmelina (Naik et al 2003), among others. For the Tabebuia genus, however, little research has focused on the breeding, domestication, or production of varietals.…”

Section: Introductionmentioning

confidence: 98%

Adventitious shoot induction from adult tissues of the tropical timber tree yellow Ipé primavera (Tabebuia donnell-smithii rose [bignoniaceae])

González-Rodríguez

Ramírez-Garduza

Robert

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Tabebuia donnell-smithii is a tropical tree species that is highly important as a forest crop due to both environmental and economic benefits. Wood from these trees is in high demand, achieving up to three times the price of wood from Pinus species. Commercial plantations of this species help to reduce the pressure on natural populations; however, plantations are few in number due to the lack of domesticated varieties and the relative unavailability of seeds. Therefore, there is an urgent need for a reliable source of plantlets suitable for commercial production. In the present work, we report the clonal propagation of T. donnell-smithii from twigs collected from elite trees. Emerging axillary shoots were used as a source of explants. Fungal contamination was a persistent problem but was partially overcome by brief exposure to low concentrations of chlorine. The stem fragments that were used as an explant source were cultured in woody plant medium containing 30 μM zeatin. This was the best condition for inducing adventitious shoot proliferation, producing 2.8 shoots per explant. Coconut water had an additional positive effect, increasing the number of shoots per explant by a factor of up to 3.6. The shoots were rooted in medium containing 20 μM indole-3-butyric acid. Ninety-four percent of the rooted plants survived the transfer to soil.

show abstract

Section: Introductionmentioning

confidence: 98%

Adventitious shoot induction from adult tissues of the tropical timber tree yellow Ipé primavera (Tabebuia donnell-smithii rose [bignoniaceae])

González-Rodríguez

Ramírez-Garduza

Robert

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

show abstract

“…A large amount of the pro-embryogenic mass was a prerequisite for the subsequent proliferation and high-frequency differentiation of somatic embryos (Parrott, 2002;Chaturvedi et al, 2003;Chaturdevi et al, 2004;Thomas and Chaturvedi, 2008). The primary somatic embryos were observed in the periphery mass after 30 days; those embryos were next to the mass or attached through a suspensor, which revealed the origin of the somatic embryos that can be multicellular or unicellular (Parrott, 2002;Morimoto et al, 2006;Singh and Chaturvedi, 2009). Therefore, A. indica developed primary somatic embryos with only BAP at 1 mg l -1 (Chaturvedi et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…at 0.1 mg l -1 TDZ (Daquinta et al, 2005) and Actinidia On the other hand, the direct somatic embryogenesis has been obtained from shoots with only cytokinins; however, there are differences between primary and secondary embryos using 1 mg l -1 BAP, or combined with auxins or other cytokinins (Te-Chato and Rungnoi, 2000;Giagnacovo et al, 2001;Chaturvedi et al, 2004;Morimoto et al, 2006). Moreover, other authors indicated that the use of low concentrations of auxins promoted regenerative organogenic callus and high concentrations induced the formation of non-regenerative calli in A. indica (Wen Su et al, 1997;Kota et al, 2006;Shekhawat et al, 2009;Das, 2011) and Melia azedarach (Deb, 2001;Sharry and Teixeira, 2006).…”

Section: Discussionmentioning

confidence: 99%

Caracterización morfo-anatómica de la embriogénesis somática secundaria en Azadirachta indica (Meliaceae)

Ramírez

Silva

2018

Acta Bot. Mex.

View full text Add to dashboard Cite

Background and Aims: Meliaceae species are extremely recalcitrant during germination and in vitro processes. Therefore, this research focuses on characterization and optimization of a highly efficient system by secondary somatic embryogenesis in Azadirachta indica, which is an important step for enhancing secondary metabolite production and regeneration in recalcitrant species.Material and Methods: Leaf and cotyledon sections were induced in MS medium supplemented with benzylaminopurine (BAP) alone, or combined with 1-naphthaleneacetic acid (NAA) and, abscisic acid (BA) with thidiazuron (TDZ).Key results: Azadirachta indica developed primary somatic embryos with BAP. Shoot and root formation occurred at low concentrations of BAP, while somatic embryogenesis was favored under high levels of BAP or TDZ. Primary and secondary somatic embryos were evidenced continuously and asynchronously. The highest amount of somatic embryos was obtained with cytokinins. However, the concentration might be significant to differentiate between primary and secondary embryos. Moreover, the auxins are key for inducing histodifferentiation in embryos. Shoot induction occurred after transfer of the embryos to hormone-free MS medium. The shoots were rooted in MS1/2.Conclusions: The secondary somatic embryos were distinguished and characterized during the whole process and the efficient system was established with cotyledon sections at short term, which offers several advantages such as the production of metabolites.

show abstract

“…Mientras que por embriogénesis somática se ha utilizado con hojas, solo citocininas, diferenciándose embriones somáticos primarios y secundarios empleando 1 mg.L -1 BAP, o combinándose con auxinas u otras citocininas en Azadirachta indica (Chaturvedi et al, 2004;Kota et al, 2006) y Azadirachta excelsa (Te-Chato et al, 2000;Morimoto et al, 2006). Por otra parte, Shekhawat et al (2009), en Azadirachta indica señalan que el desarrollo del callo embriogénico en hojas puede ser difícil cuando hay combinaciones de auxinas como 2,4-D o AIA con citocininas como el BAP, demostrando que al tener concentraciones altas de ambos reguladores de crecimiento, la frecuencia de formación de este tipo de callo se ve mermada, tal como se observó en nuestros resultados.…”

Section: En Melia Azedarachunclassified

ESTABLECIMIENTO DEL SISTEMA DE REGENERACION POR EMBRIOGÉNESIS SOMÁTICA DE Azadirachta indica A. Juss.

Ramírez¹,

Silva²

2014

Acta biol. Colomb.

View full text Add to dashboard Cite

Azadirachta indica, es una planta con múltiples aplicaciones tanto forestal como farmacológica. Por ende, el establecimiento del sistema de cultivo in vitro por embriogénesis somática ofrece diversas y variadas ventajas, tales como obtener plantas altamente productivas en metabolitos. En este estudio, se utilizaron secciones foliares y cotiledonares, inducidas en medios MS (1962) suplementados con: BAP sólo y combinado con ANA/2,4-D, TDZ sólo y con ABA. La regeneración fue con MS sólo o con K + AIA y BAP + AIA. Como resultado se estableció un sistema eficiente con secciones de cotiledones, observándose organogénesis a bajas concentraciones de BAP, mientras a altos niveles de BAP (2,5 mg.L-1), así como con TDZ + ABA (0,02 + 1mg.L-1) respectivamente favorecieron la embriogénesis somática primaria y secundaria en un 96 % y 71 % respectivamente. La regeneración fue 71 % con MS, mientras que el enraizamiento fue de 86,67 % con MS½, obteniéndose plantas completas a corto plazo. ABSTRACTAzadirachta indica, is a plant with multiple forest and pharmacological application. Therefore, the establishment of in vitro culture system for somatic embryogenesis offers several distinct advantages such as obtaining highly productive plant metabolites. In this study, were used sections cotyledon and leaf, induced on MS medium (1962) supplemented with: BAP alone and combined with NAA / 2,4-D, TDZ alone and ABA. Regeneration was with MS alone or with K + BAP + IAA and IAA. As a result was established an efficient system with cotyledon sections, being observed organogenesis at low concentrations of BAP, while high levels of BAP (2.5 mg.L-1) with 96 % and TDZ + ABA (0.02 + 1mg.L-1) with 71 %, favoring the primary and secondary somatic embryogenesis. Regeneration was 71 % with MS; rooting was 86.67 % with MS½, presenting whole plants obtained short term.RESUMOAzadirachta indica, é uma planta com várias aplicaçãos florestas e farmacológicas. Por conseguinte, a criação de sistema de cultura in vitro para a embriogénese somática apresenta várias vantagens, tais como a obtenção de plantas altamente produtivas de metabolitos. Neste estudo, as seções de cotilédones e folhas, induzidas em meios suplementado en MS (1962) con: BAP sozinho e combinado com ANA / 2,4-D, TDZ e ABA sozinho. A regeneração foi apenas com MS ou com K + BAP + IAA e IAA. Como resultado, foi eficiente con seções cotiledones, observados organogênese em baixas concentrações de BAP, en quanto altos níveis de BAP (2,5 mg L-1) e com TDZ + ABA (0,02 + 1mg.L-1) favoreceu a embriogênese somática primária e secundária até 96 % e 71%, respectivamente. Regeneração estava com MS 71 %, en quanto que o enraizamento foi de 86,67 %, com ½ MS produzindo plantas inteiras curto prazo.

show abstract

Regeneration and genetic engineering of a tropical tree, Azadirachta excelsa

Cited by 6 publications

References 20 publications

Adventitious shoot induction from adult tissues of the tropical timber tree yellow Ipé primavera (Tabebuia donnell-smithii rose [bignoniaceae])

Adventitious shoot induction from adult tissues of the tropical timber tree yellow Ipé primavera (Tabebuia donnell-smithii rose [bignoniaceae])

Caracterización morfo-anatómica de la embriogénesis somática secundaria en Azadirachta indica (Meliaceae)

ESTABLECIMIENTO DEL SISTEMA DE REGENERACION POR EMBRIOGÉNESIS SOMÁTICA DE Azadirachta indica A. Juss.

Contact Info

Product

Resources

About