Regeneration and &lt;i&gt;In Vitro&lt;/i&gt; Flowering in &lt;i&gt;Brassica Campestris&lt;/i&gt; (L.) Var. &lt;i&gt;Bhavani&lt;/i&gt;

Verma, Rinki; Singh, RR

doi:10.3126/on.v5i1.793

Cited by 12 publications

(5 citation statements)

References 10 publications

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“…This result revealed that regenerated shoots may synthesize flower inducing hormone in their body and induced flowering hormones spontaneously. Similar results on in vitro flowering of B. campestris and cauliflower was also reported by Verma andSing (2007), Vandana et al (1995) and Mollika et al 2011. Plantlets of Tori-7 were successfully transplanted into small plastic pots after adequate development of roots and the mean percentage of survival rate of the transplanted plantlets was 38.40 (Fig.…”

Section: Resultssupporting

confidence: 85%

Establishment of an Efficient in vitro Regeneration Protocol for Brassica campestris L. Var. Tori-7

Toma¹,

Biswas²,

Akhter³

et al. 2022

Plant Tissue Cult. & Biotech.

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The aim of the present investigation was to develop an efficient and reproducible in vitro regeneration protocol for Brassica campestris L. var. Tori-7. Brassica is one of the most popular and major oil yielding crop plants around the world including Bangladesh. For in vitro regeneration of this crop plant, MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) and naphthalene acetic acid (NAA) were used. The highest frequency of callus and shoot regeneration was obtained on MS medium supplemented with 3.0 mg/l BAP and 0.2 mg/l NAA from both cotyledonary leaf with petiole and hypocotyl explants. Elongation of shoots was also optimized on the same media composition. For inducing roots from the excised in vitro derived shoots, half strength of MS medium supplemented with 0.3 mg/l indole-3-butyric acid (IBA) was found to be more effective than other hormonal supplements applied. Following the development of effective roots, in vitro raised plantlets were successfully transplanted into soil where they produced flowers and fertile seeds. The plants of R1 generation was successfully established in natural environment. In vitro regeneration protocol of Brassica campestris var. Tori-7 established in this study could be used for future plant genetic transformation experiments. Plant Tissue Cult. & Biotech. 32(2): 237-246, 2022 (December)

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Section: Resultssupporting

confidence: 85%

Establishment of an Efficient in vitro Regeneration Protocol for Brassica campestris L. Var. Tori-7

Toma¹,

Biswas²,

Akhter³

et al. 2022

Plant Tissue Cult. & Biotech.

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“…It revealed that regenerated shoots synthesized flower inducing hormone in their body and induced flowering spontaneously without any external flowering hormonal supplement. The in vitro flowering of B. campestris was also reported by Verma and Singh (2007). Sarker et al (2012) reported the formation of in vitro flowers and seeds in case of lentil.…”

Section: Resultsmentioning

confidence: 54%

“…The second highest multiple shoot regenerating medium was found MS medium supplemented with1.0 mg/l BAP + 1.0 mg/l Kn +1.0 mg/l IAA using hypocotyl explants (Table I) for all three varieties of B. campestris. (Verma and Singh, 2007;Singh et al, 2011) also reported similar findings. Banu et al (2017) also observed better response in Gynura procumbens on MS with BAP and Kn supplemented medium.…”

Section: Resultsmentioning

confidence: 67%

In vitro regeneration of three varieties of <i>Brassica campestris L.</i> grown in Bangladesh

Goswami

Hoque

Khan

et al. 2020

Bangladesh J. Sci. Ind. Res.

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A reproducible in vitro regeneration system was developed for three varieties of Brassica campestris L. namely Agrani, BINA Sarisha-10 and BINA Sarisha-6 using hypocotyl and cotyledonary leaf with petiole as explants. MS medium supplemented with 2.0 mg/l BAP and 0.2 mg/l IAA was found to be the best for the multiple shoot formation for all the three varieties. Among three varieties, BINA Sarisha-6 showed best response in terms of shoot regeneration as well as number of shoots per explant (9.0) using hypocotyls as explants. In case of Agrani and BINA Sarisha-10 highest number of shoot per explants were found 8.2 and 7.0, respectively. Interestingly in vitro regenerated shoots of BINA Sarisha-6 and BINA Sarisha-10 were produced in vitro flowers on shoot regeneration media. Best root induction in BINA Sarisha-6, Agrani and BINA Sarisha-10 was achieved on MS media supplemented with 0.5 mg/l IBA. After proper hardening, the in vitro regenerated plantlets were successfully transplanted into soil. Bangladesh J. Sci. Ind. Res.55(3), 181-188, 2020

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“…Both strongly affected the flower bud induction. Maximum (Verma and Singh 2007). Exogenous hormonal supply might have been added up to the endogenous contents, raising the hormonal level required for triggering the flowering.…”

Section: Resultsmentioning

confidence: 99%

Plant growth regulators, adenine sulfate and carbohydrates regulate organogenesis and in vitro flowering of Anethum graveolens

Jana

Shekhawat

2010

Acta Physiol Plant

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In vitro regeneration protocol for Anethum graveolens (Apiaceae) was developed using leaf explants. MS basal medium used in experiments was augmented with various hormones for caulogenic and rhizogenic response. The optimum callus induction (100%) was obtained by leaf explants on MS media fortified with BA (0.5 mg l -1 ) singly and in combination with NAA (0.1 and 0.2 mg l -1 ). BA at 0.5 mg l -1 , KN at 1.0 mg l -1 and NAA at 0.1 mg l -1 induced highest number of multiple shoots (10.0 ± 0.25) per explant and they also showed in vitro flowering within 3 weeks of culture. Influence of adenine sulfate on regeneration frequency of callus was evaluated. The highest frequency of rooting (100%) with 6.0 ± 0.25 roots per explants was obtained in one-fourth strength MS medium supplemented with 1/4 MS ? IBA 0.5 mg l -1 within 4 weeks of transfer to the rooting medium. In vitro flowering (35%) was obtained with MS fortified with BA alone and also in combination with KN and NAA (5.3 ± 0.42 flowers per explants). In vitro flowering response was tested with different carbohydrates (fructose, glucose, mannose and sorbitol) and optimized. Hardening was successfully attained under controlled conditions inside the plant tissue culture room. The proposed method could effectively be applied for the conservation and clonal propagation to meet the pharmaceutical demands of this medicinally important species.

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Regeneration and <i>In Vitro</i> Flowering in <i>Brassica Campestris</i> (L.) Var. <i>Bhavani</i>

Cited by 12 publications

References 10 publications

Establishment of an Efficient in vitro Regeneration Protocol for Brassica campestris L. Var. Tori-7

Establishment of an Efficient in vitro Regeneration Protocol for Brassica campestris L. Var. Tori-7

In vitro regeneration of three varieties of <i>Brassica campestris L.</i> grown in Bangladesh

Plant growth regulators, adenine sulfate and carbohydrates regulate organogenesis and in vitro flowering of Anethum graveolens

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