Regeneration-associated cells improve recovery from myocardial infarction through enhanced vasculogenesis, anti-inflammation, and cardiomyogenesis

Salybekov, Amankeldi A.; Kawaguchi, Akira T.; Masuda, Haruchika; Vorateera, Kosit; Okada, Chisa; Asahara, Takayuki

doi:10.1371/journal.pone.0203244

Cited by 26 publications

(60 citation statements)

References 56 publications

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“…Sigma-Aldrich, St. Louis, MO, USA), as previously reported 12,13 . Briefly, immediately after centrifugation (400 g for 30 minutes at 20 °C), the mononuclear cell layer was carefully transferred to a new tube and further centrifuged (960 g for 20 minutes at 12 °C).…”

Section: Specimen Collection and Pbmcs Isolationmentioning

confidence: 99%

“…Cell phenotype was evaluated by flow cytometry as reported elsewhere 12,13 , using the following antibodies…”

Section: Cell Phenotype Characterizationmentioning

confidence: 99%

“…The number of adherent colonies was counted between days 16-18, using a gridded scoring dish (STEMCELL Tech., Vancouver, Canada) under a phase-contrast light microscope (Eclipse TE3000; Nikon, Tokyo, Japan). Primitive EPC colony-forming units (PEPC-CFUs) and definitive EPC colonyforming units (DEPC-CFUs) were separately counted as reported elsewhere 12,13 .…”

Section: Epc Colony-forming Assaymentioning

confidence: 99%

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Peripheral Levels of EPCs, T Cells, and Macrophages’ as Possible Predictive Biomarkers of Acute Myocardial Infarction

Salybekov¹,

Sakai²,

Natsumeda³

et al. 2020

Preprint

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Acute myocardial infarction (AMI), with a very relevant global disease burden, remains the major mortality and morbidity cause among all cardiovascular diseases. Patient prognosis is strictly dependent on early diagnosis and the adoption of adequate interventions. AMI diagnosis requires constant optimization, particularly considering the individuals at higher risk (or more vulnerable to worse outcomes) such as patients with diabetes mellitus and atherosclerosis. Herein, we investigated the levels of peripheral blood EPCs and immune cell-subsets from myeloid and lymphoid lineages, as well as their temporal dynamics, in the quest for new prognostic biomarkers of AMI. We collected blood from 18 hospitalized patients (days 3 and 7 after AMI onset) and 16 healthy volunteers, and resolved their circulating PBMC populations via flow cytometry. Overall, our data demonstrate a significant decrease in peripheral EPCs and CD8+ T cells, three days following an AMI. EPCs appear to be functionally impaired in AMI patients, and their circulating numbers associate with cardiac vessel lesions. Furthermore, CD8+ T cells (and even M1-macrophages) in the periphery, in combination with the classical laboratory determinations, may serve as high accuracy biomarkers of AMI, potentially aiding to prevent worse AMI outcomes.

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Section: Specimen Collection and Pbmcs Isolationmentioning

confidence: 99%

“…Cell phenotype was evaluated by flow cytometry as reported elsewhere 12,13 , using the following antibodies…”

Section: Cell Phenotype Characterizationmentioning

confidence: 99%

Section: Epc Colony-forming Assaymentioning

confidence: 99%

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Peripheral Levels of EPCs, T Cells, and Macrophages’ as Possible Predictive Biomarkers of Acute Myocardial Infarction

Salybekov¹,

Sakai²,

Natsumeda³

et al. 2020

Preprint

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“…Sca-1 can promote adhesion and proliferation of EPCs, which play a key role in optimal hematopoietic activity 18 . Upregulated Sca-1 level in EPCs could improve cardiac function recovery from myocardial infarction in rats 19 , indicating to some extent that Sca-1 is directly or indirectly involved in the regulation of EPCs.…”

Section: Introductionmentioning

confidence: 97%

Cigarette smoke extract affects methylation status and attenuates Sca-1 expression of mouse endothelial progenitor cell <i>in vitro</i>

He¹,

Chen²,

Chen³

et al. 2021

Tob. Induc. Dis.

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INTRODUCTION Endothelial dysfunction appears in many smoking-related diseases, it is also an important pathophysiological feature. Endothelial progenitor cells (EPCs) are precursors of endothelial cells and have a crucial effect on the repair and maintenance of endothelial integrity. Sca-1 is not only common in bone marrow-derived hematopoietic stem cells (HSCs), but it is also expressed in nonhematopoietic organs by tissue-resident stem and progenitor cells. The aim of this study is to investigate the impact of cigarette smoke extract (CSE) on the function of bone marrow-derived EPCs and the expression level of Sca-1 in EPCs, and also whether the methylation of Sca-1 is involved in EPC dysfunction. METHODS We measured EPC capacities including adhesion, secretion and proliferation, the concentration of endothelial nitric oxide synthase (eNOS) and apoptosis-inducing factor (AIF) in cell culture supernatant, and also Sca-1 expression and promoter methylation in EPCs induced by CSE. Decitabine (Dec) was applied to test whether it could alter the impact caused by CSE. RESULTS The adhesion, proliferation and secretion ability of EPCs can be induced to be decreased by CSE in vitro , accompanied by decreased concentrations of AIF and eNOS in cell culture supernatant and decreased Sca-1 expression in EPCs. In addition, Dec could partly attenuate the impact described above. There were no significant differences in the quantitative analysis of Sca-1 promoter methylation among different groups. CONCLUSIONS The decreased Sca-1 expression was related to EPC dysfunction induced by CSE. EPC dysfunction resulting from CSE may be related to methylation mechanism, but not the methylation of Sca-1 promoter.

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“…endothelial progenitor cells (ePcs) play a pivotal role in neoangiogenesis and mediate recovery and repair of damaged endothelium (1)(2)(3)(4). a meta-analysis of cell-based therapies in patients with refractory angina found improvements in cardiovascular outcome (5).…”

Section: Introductionmentioning

confidence: 99%

Differentiation potential and functional properties of a CD34‑CD133+ subpopulation of endothelial progenitor cells

2019

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endothelial progenitor cells (ePcs) promote angiogenesis and play an important role in myocardial and vascular repair after ischemia and infarction. ePcs consist of different subpopulations including cd34-cd133 + ePcs, which are precursors of more mature cd34 + cd133 + ePcs and functionally more active in terms of homing and endothelial regeneration. in the present study we analyzed the functional and differentiation abilities of cd34-cd133 + ePcs. isolation of ePc populations (cd34 + cd133 + , cd34-cd133 +) were performed by specific multi-step magnetic depletion. After specific stimulation a significant higher adhesive and migrative capacity of cd34-cd133 + cells could be detected compared to cd34 + cd133 + cells (P<0.001, respectively). Next to this finding, not only significantly higher rates of proliferation (P<0.005) were detected among cd34-cd133 + cells, but also a higher potential of cell-differentiation capacity into other cell types. next to a significant increase of CD34-cd133 + ePcs differentiating into a fibroblast cell-type (P<0.001), an enhancement into a hepatocytic cell-type (P=0.033) and a neural cell-type (P=0.016) could be measured in contrast to cd34 + cd133 + cells. On the other hand, there was no significant difference in differentiation into a cardiomyocyte cell-type between these ePc subpopulations (P= 0.053). These results demonstrate that ePc subpopulations vary in their functional abilities and, to different degrees, have the capacity to transdifferentiate into unrelated cell-types such as fibroblasts, hepatocytes, and neurocytes. This indicates that cd34-cd133 + cells are more pluripotent compared to the cd34 + cd133 + ePc subset, which may have important consequences for the therapy of vascular diseases.

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Regeneration-associated cells improve recovery from myocardial infarction through enhanced vasculogenesis, anti-inflammation, and cardiomyogenesis

Cited by 26 publications

References 56 publications

Peripheral Levels of EPCs, T Cells, and Macrophages’ as Possible Predictive Biomarkers of Acute Myocardial Infarction

Peripheral Levels of EPCs, T Cells, and Macrophages’ as Possible Predictive Biomarkers of Acute Myocardial Infarction

Cigarette smoke extract affects methylation status and attenuates Sca-1 expression of mouse endothelial progenitor cell <i>in vitro</i>

Differentiation potential and functional properties of a CD34‑CD133+ subpopulation of endothelial progenitor cells

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