Regeneration effect of visible light‐curing furfuryl alginate compound by release of epidermal growth factor for wound healing application

Abstract

A visible light‐curable polysaccharide conjugate was prepared and was included with epidermal growth factor (EGF) for wound healing application. Alginate was modified with furfurylamine, and the furfuryl alginate (F‐alginate) was mixed with Rose Bengal for visible light reactivity. The introduction of furfuryl group was confirmed by 1H‐NMR and UV/vis spectroscopy. Visible light‐induced crosslinking was evaluated by micropatterning and weight measurement. Swelling ratio was also determined. To verify its applicability in wound healing applications, EGF was included in the system of F‐alginate and Rose Bengal. After the confirmation of no cytotoxicity of F‐alginate and murine EGF release from the system, cell proliferation, antibacterial activity, and animal test were performed on the system. In conclusion, it was demonstrated that the system was useful for wound healing. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40113.

Bio-sourced monomers and cationic photopolymerization–The green combination towards eco-friendly and non-toxic materials

“…Owing to the high reactivity of furan derivatives, the synthesis of high-added value polymers is expected. Some examples of bio-based polymers such as DNA [415], gelatin [416], chitosan [417,418], alginate [419,420], and hyaluronic acid [421] have been previously described and are mainly used for tissue engineering applications [422], wound healing dressings [418,419,423], bio-sealant [412], antiadhesion material [416,420], regenerative medicine [424,425] and as cell laden hydrogel for 3D bioprinting [422,426].…”

Photopolymerization of Bio-Based Polymers in a Biomedical Engineering Perspective

“…Alginate was also used to create biomaterials with the opposite effect, of anti-adhesiveness, as reported by several authors. ,, In this regard, alginate was coupled with furfurylamine and further gelation took place in the presence of Rose Bengal or riboflavin, under visible light. The curing systems can serve for the immobilization of epidermal growth factor or for fluorescein isothiocyanate–bovine serum albumin as model drug . Immobilization of the epidermal growth factor promotes cell growth, proliferation, and differentiation and facilitates wound healing .…”

“…The curing systems can serve for the immobilization of epidermal growth factor or for fluorescein isothiocyanate–bovine serum albumin as model drug . Immobilization of the epidermal growth factor promotes cell growth, proliferation, and differentiation and facilitates wound healing . The application of alginate as a physical barrier fits with its natural poor cell adhesion and film forming capacity.…”

In situ light‐activated materials for skin wound healing and repair: A narrative review

“…The authors found a slight acceleration in epithelialization mediated by the O ‐CMC/FGE base material, encapsulation with mEGF reduced foreign body reaction to the material and enhanced fibroblast activity in the healing wound. In a complementary study, Heo et al conjugated furfurylamine to alginate, resulting in furfuryl alginate (F‐alginate), which was combined with 0.5% wt/vol Rose Bengal for visible light reactivity 80 . Full‐thickness non‐splinted wounds were generated in Sprague Dawley rats, and F‐alginate with or without mEGF was photocured in situ with a dental curing light (420–490 nm) for 7 min.…”

“…In a complementary study, Heo et al conjugated furfurylamine to alginate, resulting in furfuryl alginate (F‐alginate), which was combined with 0.5% wt/vol Rose Bengal for visible light reactivity. 80 Full‐thickness non‐splinted wounds were generated in Sprague Dawley rats, and F‐alginate with or without mEGF was photocured in situ with a dental curing light (420–490 nm) for 7 min. The authors found enhanced epithelialization and fibroblast activity that was further augmented by the incorporation of mEGF, with the F‐alginate acting to limit foreign body responses.…”