Regeneration in free grafts of normal and denervated muscles in the rat: Morphology and histochemistry

Abstract

Intact soleus and extensor digitorum longus muscles in the rat were freely grafted to the contralateral leg after either no preliminary treatment or 14 days prior denervation. Normal muscle grafts during the first week were characterized by a central zone of degenerating original muscle fibers (disappearing by 7-9 days) and a peripheral zone, containing regenerating muscle as well as small numbers of surviving original muscle fibers. A radial gradient of regeneration was establihed, with more mature muscle at the periphery and less mature muscle toward the center. Denervated grafts were characterized by rapid degeneration (within 2-3 days) of original muscle fibers in the central area, rapid appearance of regenerating muscle fibers (e.g. cross striations by 5 days) with uniform levels of differentiation throughout the graft and larger numbers of surviving original muscle fibers at the periphery. During the first week, stages of muscle differentiation in denervated grafts were attained 1-2 days earlier than comparable stages in normal grafts. Later stages of muscle differentiation were similar in both types of grafts. Histochemical studies revealed a loss of enzyme activity (phosphorylase, ATPase and SDH) in the center of early (2-4-day) normal and denervated grafts. Denervated grafts, however, possessed a thicker peripheral rim of enzymatically active surviving muscle fibers than normal grafts. In both types of grafts the old muscle fibers in the center were replaced by enzymatically active regenerating muscle fibers which stained uniformaly (ATPase) until 30 days. By 60 days a mixed fiber pattern had developed. Muscle spindles were found within the grafts.

The physiology, pharmacology, and trophic effectiveness of synapses formed by autonomic preganglionic nerves on frog skeletal muscle.

“…However, the persistence of cholinesterase deposits at old end-plates, the ability of the muscle to contract to direct stimulation at all times after transplantation, and the histological finding that individual fibres continued to look normal throughout the period of transplantation indicates that the fibres that remained were the original fibres, not newly differentiated ones, as might occur in transplanted mammalian muscle (Carlson & Gutmann, 1975). The action potentials of long-term denervated muscle fibres, like those of control fibres, were quickly and completely blocked by 10-s M-tetrodotoxin.…”

Low-Molecular-Weight Fucoidan Promotes Therapeutic Revascularization in a Rat Model of Critical Hindlimb Ischemia

“…at ASPET Journals on May 12, 2018 jpet.aspetjournals.org stained with 1% lugol substrate to study phosphorylase activity (Carlson and Gutmann, 1975). Phosphorylase activity indicates muscle glycogenolysis, and metabolically active skeletal muscle fibers stain brown, whereas a yellow stain indicates absence of metabolic activity (Carlson and Gutmann, 1975).…”

Limitations of nlsβ‐galactosidase as a marker for studying myogenic lineage or the efficacy of myoblast transfer

“…Following whole-muscle transplantation, most of the myofibers (with the exception of peripheral myofibers of the graft) undergo necrosis (Carlson and Gutmann, 1975). Within a week after transplantation, the entire girth of the mouse EDL muscle is filled with regenerated myofibers (formed from the muscle's myosatellite cells, which become activated by the trauma of transplantation; Thomas et al, 1984).…”