Regeneration in Jatropha curcas: Factors affecting the efficiency of in vitro regeneration

Sharma, Sweta; Kumar, Nitish; Reddy, M. Venkat Ram

doi:10.1016/j.indcrop.2011.02.017

Cited by 43 publications

(30 citation statements)

References 49 publications

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“…In the present study, TDZ plays an important role in the callus induction as well as regeneration. These results were in accordance with Sharma et al, 2011(Sharma et al, 2011. The callus induction was also promoted by the combination of cytokinin and auxins (Capuana et al, 2007).…”

Section: Discussionsupporting

confidence: 86%

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

Elangomathavan¹,

S²,

Hariharan³

et al. 2017

Int. J. Adv. Res. Biol. Sci

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In this study an effective plant regeneration protocol for multiple shoot bud induction was optimized for the micropropagation of plantlets through indirect organogenesis from leaf explants of C. collinus. The young leaves were cultured on Murashige and Skoog (MS) medium with Thidiazuron (TDZ), 6-Benzylaminopurine (BAP), Kinetin (KIN), 2,4-Dichlorophenoxyacetic acid (2,4-D) and 1-Naphthaleneacetic acid (NAA) at 0.5-3.0 mg/l for callus induction. The induced calli was excised and subcultured on MS medium supplemented with BAP, KIN and TDZ (0.5-4.0 mg/l) in combination with NAA (0.5 mg/l) to find out the optimum hormone concentration for shoot bud induction. For rooting, half strength MS medium with various concentrations (0.5-3.0 mg/l) of Indole 3-acetic acid (IAA), Indole 3-butyric acid (IBA) and NAA was used. Initially, a creamy white callus was formed from the cut ends of leaf explants and gradually these mass of unorganised cells transformed to light green regenerable callus. The maximum callus induction (100%) was observed in MS medium with TDZ at 1.0 mg/l concentration. The highest frequency of organogenesis was observed in the combination of BAP (1.0 mg/l) and NAA (0.5 mg/l) with a mean number of 12.1±1.1 shoots followed by TDZ (1.0 mg/l) and NAA (0.5 mg/l) with 10.2±1.0 shoots per explants. The healthy shoots were rooted best on half strength MS medium supplemented with 0.5mg/l IAA. The well developed plantlets with healthy roots were successfully acclimatized. The development of thriving protocol for the callus induction and regeneration would make possible for the mass multiplication and in vitro production of plantlets with somaclonal variations.

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Section: Discussionsupporting

confidence: 86%

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

Elangomathavan¹,

S²,

Hariharan³

et al. 2017

Int. J. Adv. Res. Biol. Sci

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“…ere are many reports of in vitro regeneration of plants from di erent tissues of Jatropha, including leaves (Sujatha and Mukta 1996;Sujatha et al 2005;Jha et al 2007; Deore and Johnson 2008;Khurana-Kaul et al 2010), cotyledon (Khemkladngoen et al 2011b;Kumar et al 2010a;Varshney and Johnson 2010), petioles (Sujatha and Mukta 1996;Kumar and Reddy 2010;Kumar et al 2011), hypocotyls (Sujatha and Mukta 1996;Kaewpoo and Te-chato 2010;Sharma et al 2011), epicotyls (Wei et al 2004;Kaewpoo and Te-chato 2010), nodal segments ), axillary nodes (Sujatha et al 2005), shoot tips (Rajore and Batra 2005;Purkayastha et al 2010), and stems . Based on these regeneration protocols, stable transformation of Jatropha has been reported by several groups.…”

Section: Genetic Engineering Is a Practical Technique To Improvementioning

confidence: 99%

Establishment of bispyribac selection protocols for Agrobacterium tumefaciens- and Agrobacterium rhizogenes-mediated transformation of the oil seed plant Jatropha curcas L.

Kajikawa

Morikawa

Inoue

et al. 2012

Plant Biotechnology

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Cultivation of the biofuel plant Jatropha (Jatropha curcas L.) has spread around the world because of its drought resistance, high seed oil content, and adaptability to di erent environmental conditions. Because of these attributes, Jatropha has the potential to be one of the main resources for next-generation biodiesel fuel. To improve the productivity of Jatropha biomass, it is important to understand the molecular functions of key Jatropha genes, and to modify various agronomic traits of Jatropha via molecular breeding. A reliable and e cient protocol for genetic transformation of Jatropha is a prerequisite for molecular biology research and breeding on this plant. Here, we developed a system in which the herbicide bispyribac sodium salt, which inhibits acetolactate synthase, was used as the selection agent, and a two-point-mutated acetolactate synthase gene (mALS) was used to confer resistance upon transformants. Application of this system signi cantly improved the e ciency of Agrobacterium tumefaciens-mediated stable transformation of the high-yielding elite Jatropha population, IP-2P. e bispyribac-mALS system was also successfully applied in the Agrobacterium rhizogenes-mediated hairy roots system, which allowed integration of a foreign gene and expression in Jatropha root tissues within 2 weeks. e new protocols described here are powerful tools not only for functional studies on endogenous genes, but also for the molecular breeding of Jatropha to develop elite varieties.

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“…However, two aspects restrict their domestication, seed production is erratic due to heterozygous plants and the genetic variability in germplasm collections of the species is close (Singh et al, 2010;Sharmaa et al, 2011). Seeds with high lipid content have shown reduced lifespan under storage in genebanks when conventional conservation are adopted, -18ºC (Crane et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Methods to assess the viability of cryopreserved Jatropha curcas L. seed germplasm

Salomão

Santos

José

et al. 2016

Rev. bras. plantas med.

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Jatropha curcas L. is a plant species with many potential applications, especially medicinal uses (hypoglycemic, anti-inflammatory, haemostatic, healing, anti-tumor). The objective of this study was to test germination in moist paper rolls for whole seeds and in vitro for excised embryonic axes, in an attempt to identify the best method to assess the quality of J. curcas seed germplasm, cryopreserved with different water contents. The experimental sample with a 6.2% moisture content (MC) was divided in subsamples which were hydrated and dehydrated for 0 (control), 4, 8, 11 and 24h. The initial germination percentages were 63% for whole seeds and 81% for excised embryonic axes. After exposure to liquid nitrogen (LN), germination percentages were 48% (whole seeds) and 57% (excised embryonic axes). There was no significant difference between germination percentages in embryonic excised from seeds subjected or not subjected to freezing, with different MC. In contrast, there was a reduction of the whole seed germination percentage when exposed to LN (contrast = 0.17, standard error = 0.04, t = 4.09, p = 0.001) and not for the hydration and dehydration treatments. The methodology based on in vitro cultures of the embryonic axis isolated from seeds stored in LN with distinct MC values was more efficient than the standard germination test to evaluate the viability of J. curcas seeds before and after LN storage.Keywords: seed, embryonic axis, cryopreservation, Jatropha curcas RESUMO: Métodos para avaliar a viabilidade de germoplasma semente criopreservado de Jatropha curcas L. Jatropha curcas L., é uma espécie com várias aplicações potenciais, principalmente para usos medicinais (hipoglicemina, anti-inflamatório, homeostático, cicatrizante, antitumoral). O objetivo deste estudo foi testar a germinação em rolo de papel para sementes inteiras e in vitro para eixos embrionários excisados visando identificar o melhor método para avaliar a qualidade de germoplasma semente de J. curcas, criopreservado com diferentes teores de água. A amostra experimental com 6,2% de teor de água foi dividida em subamostras que foram hidratadas e desidratadas por 0 (controle), 4, 8, 11 e 24 h. Os percentuais de germinação inicial foram de 63% para sementes inteiras e de 81% para eixos embrionários excisados. Após exposição ao nitrogênio líquido (NL) os percentuais de germinação foram de 48% (sementes inteiras) e 57% (eixos embrionários). Não houve diferença significativa entre os percentuais de germinação de eixos embrionários excisados de sementes com diferentes umidades e submetidas ou não ao congelamento. Em contraste, houve redução de percentuais de germinação das sementes inteiras expostas ao NL (contraste = 0.17, erro padrão = 0.04, t = 4.09, p = 0.001), mas não aos tratamentos de hidratação e desidratação. A metodologia baseada em cultura de eixos embrionários in vitro isolados de sementes armazenadas em NL, com distintos teores de água foi mais eficiente que a germinação padrão para avaliar a viabilidade de sementes J. curcas ...

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Regeneration in Jatropha curcas: Factors affecting the efficiency of in vitro regeneration

Cited by 43 publications

References 49 publications

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

Establishment of bispyribac selection protocols for <i>Agrobacterium tumefaciens</i>- and <i>Agrobacterium rhizogenes</i>-mediated transformation of the oil seed plant <i>Jatropha curcas</i> L.

Methods to assess the viability of cryopreserved Jatropha curcas L. seed germplasm

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