Regeneration in the central nervous system of a pulmonate mollusc, Melampus

Price, Christopher H.

doi:10.1007/bf00220173

Cited by 23 publications

(15 citation statements)

References 8 publications

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“…Previous authors have taken this latter view because the tentacle ganglion and the conventional CNS have a common embryological origin in the ectodermal cephalic plate (Samassa, 1894;Hanstrom, 1925;ChQtail, 1963). It has recently been demonstrated that the molluscan cerebral ganglia also share with the tentacle ganglion the capability of regeneration (Price, 1977;Moffett, 1980). The evaluation of functional competence involved a test of odor detection in a procedure demanding olfactory orientation behavior.…”

Section: Discuss I0 Nmentioning

confidence: 99%

Morphology and odor sensitivity of regenerated snail tentacles

Chase

Kamil

1983

J. Neurobiol.

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This study examined certain structural and functional aspects of the olfactory system in regenerated posterior tentacles of the terrestrial snail Achatina fulica. Regeneration of the epithelial sensory pad occurs with accurate size regulation. All five neuronal cell types which are normally revealed by horseradish peroxidase backfilling are also regenerated. The sensory cells attain normal numbers at about 20 weeks postlesion. The organization of neuronal elements within the tentacle is chaotic, however, at early stages of regeneration. Even later, the digitlike extensions of the ganglion, which are characteristic of intact tentacles, fail to appear. The recovery of odor sensitivity was evaluated using a tentacular olfactormeter and a behavioral assay that involved locomotor orientation towards the odor stimulus. Thresholds and concentration-dependent response rates were equivalent for regenerated and intact tentacles, tested in the same animals, at 10 weeks post-lesion.

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Section: Discuss I0 Nmentioning

confidence: 99%

Morphology and odor sensitivity of regenerated snail tentacles

Chase

Kamil

1983

J. Neurobiol.

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“…The gastropod mollusk Melampus bidentatus can regenerate functional neural connections (Moett 1992) and also is able to replace central neurons (Price 1977;Moett and Austin 1982;Moett and Ridgway 1988).…”

Section: Introductionmentioning

confidence: 99%

Neuronal and non-neuronal responses to nerve crush in a pulmonate snail, Melampus bidentatus

Bale

Howard

Moffett

2001

Invertebrate Neuroscience

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Immunohistochemical and ultrastructural techniques were used to study sequelae of nerve injury in the pulmonate snail Melampus bidentatus. Either pedal or tentacle nerves were crushed, severing all axons, and recovery was monitored over 15 days. The axons regenerated from the segment attached to the soma, with no evidence of fusion of proximal and distal segments. The medium to large axons of central neurons, including those monitored with serotonin immunohistochemistry, grow distally across the path of smaller axons extending centrally from peripheral somata. The regions into which the growing axons projected were a focus of phagocytic activity. Cells previously labeled by PKH-26PCL, a fluorescent marker for phagocytic activity, were attracted to the crushed nerve within 6 h and were a consistent feature in the vicinity of the injury for at least 9 days, gradually extending their range as repair progressed in both directions from the crush. Repair proceeded within an intact sheath, and many sheath cells survived the crush, although the nuclear dye Hoechst 33258 revealed an initial distortion of their nuclei. The concentration of cells in the sheath in the crushed region increases after the crush, with the packing of nuclei peaking at 3 days and gradually returning to control conditions; this probably reflects migration of resident sheath cells. Cell division is rare in the sheath of intact nerves, but labeling with bromodeoxyuridine increases at the crush site between 4 and 9 days, indicating that cell replacement also occurs at the site.

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“…The injured brain spontaneously regenerates its structure, and its function is restored without any exogenous treatment (Price, 1977;Moffett, 1995;Matsuo and Ito, 2010;Matsuo et al, 2010a). The most prominent example is the tentacle of terrestrial pulmonates (Stylommatophora).…”

Section: Introductionmentioning

confidence: 99%

Effects of tentacle amputation and regeneration on the morphology and activity of the olfactory center of the terrestrial slug Limax valentianus

Matsuo

Kobayashi

Tanaka

et al. 2010

Journal of Experimental Biology

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SUMMARYThe tentacles of pulmonates regenerate spontaneously following amputation. The regenerated tentacle is equipped with all the elements necessary for normal olfactory functioning, and the slugs can behave as well as they did before the tentacle amputation. However, it is not known what changes occur to the olfactory center procerebrum in the brain at the morphological and physiological levels. Here, we investigated the innervation of tentacular nerves into the procerebrum by examining the size of the terminal mass (input layer from tentacular nerves) of the procerebrum and also by staining afferent nerves immunohistochemically at 15, 58 and 75 days following unilateral amputation of the superior and inferior tentacles. The size of the terminal mass was significantly decreased, and the Phe-Met-Arg-Phe-NH 2 ergic (FMRFamidergic) afferent nerves disappeared by 15 days following the tentacle amputation. However, the size of the terminal mass had recovered substantially by 58 days, as the tentacle regenerated. The FMRFamidergic innervation into the cerebral ganglion was also restored by this time. An extended recovery (75 days), however, did not result in any further increase in the size of the terminal mass. We also recorded the local field potential (LFP) oscillation in the procerebrum. We found that the oscillatory frequency of the LFP had decreased at 15 days following the tentacle amputation but had recovered at 58 and 75 days. These results suggest that the amputation and regrowth of the tentacle are accompanied by the respective degeneration and re-innervation of olfactory nerves, and these changes in the innervation status affect the basal state of LFP oscillation.

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Regeneration in the central nervous system of a pulmonate mollusc, Melampus

Cited by 23 publications

References 8 publications

Morphology and odor sensitivity of regenerated snail tentacles

Morphology and odor sensitivity of regenerated snail tentacles

Neuronal and non-neuronal responses to nerve crush in a pulmonate snail, Melampus bidentatus

Effects of tentacle amputation and regeneration on the morphology and activity of the olfactory center of the terrestrial slug Limax valentianus

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