Regeneration of Andrographis paniculata Nees: Analysis of genetic fidelity and andrographolide content in micropropagated plants

Abstract

Andrographis paniculata Nees is a valuable medicinal plant which yields the therapeutic compound andrographolide. The objective of the present study was to develop reliable in vitro propagation techniques in this plant species. The efficiency of shoot regeneration in A. paniculata was tested on the Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP), thidiazuron (TDZ), kinetin (Kn) and 2-isopentenyl adenine (2-iP) at concentrations of 0.5, 1.0, 2.0, 5.0 and 10.0 μM and BAP (1.0 μM) in combination with other cytokinins like TDZ, Kn and 2-iP (0.5, 1.0, 2.0, 5.0 and 10.0 μM) by using nodal explants. Maximum number of 39 shoots per explant was recorded on MS medium supplemented with BAP (1.0 μM) and Kn (5.0 μM). An anatomical study confirmed shoot regeneration via direct organogenesis. Regenerated shoots were cultured on MS medium supplemented with 1naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) at concentrations of 0.5, 1.0, 2.0 and 5.0 μM for the induction of roots. Cent percent shoots developed roots after transfer to half strength MS medium supplemented with IBA (2.0 μM). The rooted plantlets were successfully acclimatized and established in soil. Randomly amplified polymorphic deoxyribonucleic acid (DNA) (RAPD) analysis was carried out to check for possible genetic alterations in regenerated plants and the results revealed that the recovered plants did not exhibit any type of polymorphism. The andrographolide content was determined in regenerated plants using high performance liquid chromatography (HPLC) and regenerated plants had considerable amount of andrographolide, so regenerated plants could be used as raw material for andrographolide extraction.

Efficient In vitro Micro Propagation of Andrographis paniculata and Evaluation of Antibacterial Activity from Its Crude Protein Extract

“…1. The results from the current study were in agreement with the findings reported by Vijayalaxmi and Hosakatte [39] which demonstrated that maximum rooting was on half strength MS medium supplemented with 2.0 μM IBA. The enhancing effect of NAA over IBA on rooting in microshoots were reported by a range of worker during in vitro micro-propagation of medicinal plant [40], which also contradictory with our present study.…”

Auxin increased adventitious root development in the medicinal plant Andrographis paniculata (Burm. f.) Wall. ex Nees

“…The performance of IBA and IAA in adventitious root development in A. paniculata was also evident in several in vitro studies. A higher auxin concentration was reported to be more effective for adventitious rooting in shoot cuttings (Dandin & Murthy, 2012; Purkayastha et al., 2008). Higher dosages of IBA were more efficient at inducing adventitious roots in A. paniculata than IAA.…”