Regeneration of articular cartilage defects: Therapeutic strategies and perspectives

Guo, Xueqiang; Ling, Xi; Yu, Mengyuan; Fan, Zhenlin; Wang, Weiyun; Ju, Andong; Liang, Zhuo; Zhou, Guangdong; Ren, Wenjie

doi:10.1177/20417314231164765

Cited by 36 publications

(16 citation statements)

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“…Osteoarthritis is a chronic, degenerative joint disease characterized by the erosion of joint cartilage and inflammation, as well as degradation of the ECM [ 156 , 157 ]. Chondrocyte is the only type of cell found in cartilage, and the activity of chondrocyte is regulated by multiple inflammatory and metabolic factors [ 158 ]. Although METTL3 may help the chondrogenesis of BMSCs, the role of this m 6 A “writer” in the development of osteoarthritis was debatable [ 159 , 160 ].…”

Section: A Modification In Musculoskeletal Disordersmentioning

confidence: 99%

N6-Methyladenosine in Cell-Fate Determination of BMSCs: From Mechanism to Applications

Zhang,

Li,

Wang

et al. 2024

Research

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The methylation of adenosine base at the nitrogen-6 position is referred to as “N6-methyladenosine (m 6 A)” and is one of the most prevalent epigenetic modifications in eukaryotic mRNA and noncoding RNA (ncRNA). Various m 6 A complex components known as “writers,” “erasers,” and “readers” are involved in the function of m 6 A. Numerous studies have demonstrated that m 6 A plays a crucial role in facilitating communication between different cell types, hence influencing the progression of diverse physiological and pathological phenomena. In recent years, a multitude of functions and molecular pathways linked to m 6 A have been identified in the osteogenic, adipogenic, and chondrogenic differentiation of bone mesenchymal stem cells (BMSCs). Nevertheless, a comprehensive summary of these findings has yet to be provided. In this review, we primarily examined the m 6 A alteration of transcripts associated with transcription factors (TFs), as well as other crucial genes and pathways that are involved in the differentiation of BMSCs. Meanwhile, the mutual interactive network between m 6 A modification, miRNAs, and lncRNAs was intensively elucidated. In the last section, given the beneficial effect of m 6 A modification in osteogenesis and chondrogenesis of BMSCs, we expounded upon the potential utility of m 6 A-related therapeutic interventions in the identification and management of human musculoskeletal disorders manifesting bone and cartilage destruction, such as osteoporosis, osteomyelitis, osteoarthritis, and bone defect.

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Section: A Modification In Musculoskeletal Disordersmentioning

confidence: 99%

N6-Methyladenosine in Cell-Fate Determination of BMSCs: From Mechanism to Applications

Zhang,

Li,

Wang

et al. 2024

Research

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“…Hyaline cartilage, also called articular cartilage is a connective tissue in nature which mainly consists of chondrocytes and extracellular matrix (ECM) containing abundant collagen fibers and proteoglycans ( Ortiz-Arrabal et al, 2021 ; Wu et al, 2021 ). The self-healing capability of damaged cartilage tissue is notably bounded due to features such as avascularity, neural and lymphatic characteristics, the poor migration ability of chondrocytes, and the small number of progenitor cells ( Ortiz-Arrabal et al, 2021 ; Guo et al, 2023 ), which may cause structural anomalies and malfunction( Redondo et al, 2018 ). Common surgical methods contain abrasion arthroplasty, drilling, and micro-fracture, but these techniques do not yield ideal results( Mobasheri et al, 2014 ), and the repair of cartilage defects is still a challenge for clinical surgeons ( Kwon et al, 2019 ; Xue et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Natural based hydrogels promote chondrogenic differentiation of human mesenchymal stem cells

Zahedi Tehrani,

Irani,

Ardeshirylajimi

et al. 2024

Front. Bioeng. Biotechnol.

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Background: The cartilage tissue lacks blood vessels, which is composed of chondrocytes and ECM. Due to this vessel-less structure, it is difficult to repair cartilage tissue damages. One of the new methods to repair cartilage damage is to use tissue engineering. In the present study, it was attempted to simulate a three-dimensional environment similar to the natural ECM of cartilage tissue by using hydrogels made of natural materials, including Chitosan and different ratios of Alginate.Material and methods: Chitosan, alginate and Chitosan/Alginate hydrogels were fabricated. Fourier Transform Infrared, XRD, swelling ratio, porosity measurement and degradation tests were applied to scaffolds characterization. After that, human adipose derived-mesenchymal stem cells (hADMSCs) were cultured on the hydrogels and then their viability and chondrogenic differentiation capacity were studied. Safranin O and Alcian blue staining, immunofluorescence staining and real time RT-PCR were used as analytical methods for chondrogenic differentiation potential evaluation of hADMSCs when cultured on the hydrogels.Results: The highest degradation rate was detected in Chitosan/Alginate (1:0.5) group The scaffold biocompatibility results revealed that the viability of the cells cultured on the hydrogels groups was not significantly different with the cells cultured in the control group. Safranin O staining, Alcian blue staining, immunofluorescence staining and real time PCR results revealed that the chondrogenic differentiation potential of the hADMSCs when grown on the Chitosan/Alginate hydrogel (1:0.5) was significantly higher than those cell grown on the other groups.Conclusion: Taken together, these results suggest that Chitosan/Alginate hydrogel (1:0.5) could be a promising candidate for cartilage tissue engineering applications.

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“…Today, conservative treatments, such as physical therapy and pain-alleviating OA drugs, are used as the first treatment for OA [ 10 , 11 ]. When the disease advances, orthopedic techniques are timely options [ 12 ]. Regenerative tissue-engineering approaches have also been investigated more and more frequently taking advantage of various types of synthetic or natural scaffolds and chondrocytes/stem cells, bioactive factors, exosomes and even gene therapies with, for instance, anabolic factors or non-coding RNAs [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Regulation of Oxygen Tension as a Strategy to Control Chondrocytic Phenotype for Cartilage Tissue Engineering and Regeneration

Lammi,

2024

Bioengineering

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Cartilage defects and osteoarthritis are health problems which are major burdens on health care systems globally, especially in aging populations. Cartilage is a vulnerable tissue, which generally faces a progressive degenerative process when injured. This makes it the 11th most common cause of global disability. Conservative methods are used to treat the initial phases of the illness, while orthopedic management is the method used for more progressed phases. These include, for instance, arthroscopic shaving, microfracturing and mosaicplasty, and joint replacement as the final treatment. Cell-based implantation methods have also been developed. Despite reports of successful treatments, they often suffer from the non-optimal nature of chondrocyte phenotype in the repair tissue. Thus, improved strategies to control the phenotype of the regenerating cells are needed. Avascular tissue cartilage relies on diffusion for nutrients acquisition and the removal of metabolic waste products. A low oxygen content is also present in cartilage, and the chondrocytes are, in fact, well adapted to it. Therefore, this raises an idea that the regulation of oxygen tension could be a strategy to control the chondrocyte phenotype expression, important in cartilage tissue for regenerative purposes. This narrative review discusses the aspects related to oxygen tension in the metabolism and regulation of articular and growth plate chondrocytes and progenitor cell phenotypes, and the role of some microenvironmental factors as regulators of chondrocytes.

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Regeneration of articular cartilage defects: Therapeutic strategies and perspectives

Cited by 36 publications

References 235 publications

N6-Methyladenosine in Cell-Fate Determination of BMSCs: From Mechanism to Applications

N6-Methyladenosine in Cell-Fate Determination of BMSCs: From Mechanism to Applications

Natural based hydrogels promote chondrogenic differentiation of human mesenchymal stem cells

Regulation of Oxygen Tension as a Strategy to Control Chondrocytic Phenotype for Cartilage Tissue Engineering and Regeneration

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