Regeneration of coconut ( Cocos nucifera L.) from plumule explants through somatic embryogenesis

Somatic Embryogenesis on Plumule and Radicle Explants Obtained from Warm Water Hydroprimed Wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

“…Although there are number of reports on the use of plumule explants in other plants like Cocos nucifera (Chan et al 1998;Hornung 1995) and sorghum (Aher ; there is no report on shoot regeneration from the plumule and radicle explants of wheat. A review of previous literature suggests variable success in tissue culture of different wheat species using callus of immature embryos (Becker et al 1994;Elena & Ginzo 1988;Maddock et al 1983;Weeks et al 1993) and mature embryos (Bi et al 2007;Patnaik et al 2006).…”

Regeneration of <i>in Vitro</i> Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos

“…In addition, they showed only 20% of browning (Table 1). Application of maltose of 1 M eliminated browning effectively compared to 0.5 M. Culturing of Primula vulgaris in half-strength MS with 0.1 M maltose was limited in browning and callus induction observed [6]. Previous reports have also found maltose to be a better carbon source for regeneration, which supplementation of 4% maltose showed best results for per cent callus induction (90.33%), embryogenic callus formation (57.66%) and shoot regeneration (82.66%) in Japonica Rice Cv.…”

Comparative gene expression profiling during in vitro regeneration in two coconut cultivars

“…Many explants have been tested, including immature infl orescence (Branton and Blake, 1984), immature embryos (Gupta et al, 1984), immature leaves (Raju et al, 1984;Buffard-Morel et al, 1992) and the results obtained indicate that regeneration can be achieved, but is rather diffi cult. Somatic embryos, developed from plumular explants, were capable of germination and subsequent development into plantlets, which could be successfully transferred to nursery (Chan et al, 1998;Rajesh et al, 2005Rajesh et al, , 2014a.…”