The recovery and survival of the Macaronesian laurel forest depends on its regeneration strategies. After years of long-term monitoring, both sexual and asexual regeneration appear to be equally important. However, the mechanisms for each are just beginning to be understood. In order to contribute to the understanding of the laurel forest sexual regeneration, we analyzed the species composition of the seedling bank every two weeks over three years in the laurel forest of Anaga (Tenerife, Canary Islands). We compared the species compositions of the seedling bank with the canopy, and analyzed changes in their diversity over this period in different forest stands. We found that species diversity (evenness) is different among plots regardless of the stand. In some cases, plot diversity remained constant over time, while others showed some variations, which were little related to climatic conditions (temperature and precipitation). We also found no relationship between the seedling bank and canopy composition, with shade-intolerant species being more abundant in the former. Although climatic conditions remained constant during the period and other environmental conditions did not vary either, some changes were found in the seedling bank species composition. These were related to the increased degree of conservation of the laurel forest of Anaga (by closing unpaved roads, limiting access, and the abandonment of agriculture) that had negatively affected the density of shade-intolerant species. We suggest that such conservation measures should be maintained and extended to other areas where agriculture has been recently abandoned to allow the potential establishment of laurel forest and late successional species.