Regeneration of Gossypium hirsutum and G. barbadense from shoot apex tissues for transformation

Gould, Jean H.; Banister, Sharon; Hasegawa, Osamu; Fahima, Michal; Smith, Roberta H.

doi:10.1007/bf00233024

Cited by 73 publications

(41 citation statements)

References 23 publications

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“…For any tissue culture study, the surface of explants must be fully sterilized. In previous research, different sterilization methods were used to sterilize delinted cotton seeds surface (Gould et al, 1991;Chen et al, 1987;Zhang, 1994). Delintedseeds were disinfected as describedabove and the disinfected seeds were then cultured on MS medium for 7 days.…”

Section: Discussionmentioning

confidence: 99%

“…Regeneration from the shoot apex was direct and simple. Theoretically, each excised apex should develop into a rooted plant; however, the yield of shoots in vitro from isolated apices depends on the incidence of contamination and rooting efficiency (Gould et al, 1991). In recent years, protocols involving proliferation of cotton shoots (Agrawal et al, 1997;Hemphill et al, 1998) have been published.…”

Section: Discussionmentioning

confidence: 99%

“…For shoot elongation and root development, isolated shoot apices from the three different cotton varieties: SAMCOT-9, 11 and 13, were placed on MS medium+0.1 mg/L Kinetin (Gould et al, 1991) for two weeks to induce shoot elongation. The shoots were then transferred to MS medium for rooting.…”

Section: Post-transformational Analysismentioning

confidence: 99%

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Expression of Mannose-Binding Insecticidal Lectin Gene in Transgenic Cotton (<i>Gossypium</i>) Plant

N.B.

H.M.²,

I.³

et al. 2011

cgg

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Cotton (Gossypium spp) is an important world crop. Despite the efforts made through traditional breeding methods, cotton breeders still faced with many problems, i.e., narrow genetic base, inability to use alien genes and difficulty in breaking gene linkages. Breeders attempted genetic transformations analyses tools to overcome these problems with very little success, hence the need for transgenic intervention. In this report, an optimized cotton regeneration system from shoot apices used to transform cotton wit insecticidal lectin gene from Allium sativum.Cotton regeneration system was observed to be genotype independent with a regeneration rate of 85% obtained within 16 weeks. The age of explants and the size of isolated tips have a significant effect on shoot tip elongation. The elongation rates of the three varieties were not significantly different from each other (p=0.157 3). It was observed that Samcot 9 had the highest rooting efficiency (47%), and Samcot 13 had the least rooting efficiency (36%). Though the difference in rooting efficiency was not significant in the three varieties (P=0.08). Transgenic cotton plants were obtained via Agrobacterium-mediated transformation using shoot apices as explants. Transformation rate was 1.3% using LBA 4404 with β-glucuronidase (GUS) gene. The mean number of GUS positive apices was 67% higher when acetosyringone was included in the medium. Agrobacterium concentration and co-cultivation time have a significant effect on transient GUS expression. The highest GUS positive number was observed at OD 600 0.6 and co-cultivation for 3 days. Putative transgenic plants were confirmed by leaf GUS assay, kanamycin leaf test and molecular analysis of putative young leaves.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Expression of Mannose-Binding Insecticidal Lectin Gene in Transgenic Cotton (<i>Gossypium</i>) Plant

N.B.

H.M.²,

I.³

et al. 2011

cgg

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“…The advantage of using the shoot apex for Agrobacterium-mediated gene transformation is a low incidence of tissue culture induced genetic changes, and a simple and direct development of transformed plants (Gould et al 1991). Recently the shoot apex method has been used successfully in Agrobacterium-mediated transformation of rice (Clement et al 2016), cucumber (Baskaran et al 2016), and broccoli (Ravanfar & Abdul Aziz 2015) (Table 1).…”

Section: Shoot Apex Methodsmentioning

confidence: 99%

Tissue culture-based Agrobacterium-mediated and in planta transformation methods

Niazian¹,

Noori²,

Galuszka³

et al. 2017

Czech J. Genet. Plant Breed.

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Niazian M., Sadat Noori S.A., Galuszka P., Mortazavian S.M.M. (2017): Tissue culture-based Agrobacterium-mediated and in planta transformation methods. Czech J. Genet. Plant Breed., 53: 133−143.Gene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is the Agrobacterium-mediated method. The main problem of this method is that some plant species and mutant lines are recalcitrant to regeneration. Requirements for sterile conditions for plant regeneration are another problem of Agrobacterium-mediated transformation. The development of a genotype-independent gene transformation method is of great interest in many plants. Some Agrobacteriummediated gene transformation methods independent of tissue culture are reported in individual plants and crops. Generally, these methods are called in planta gene transformation. In planta transformation methods are free from somaclonal variation and easier, quicker, and simpler than transformation methods based on tissue culture. Vacuum infiltration, injection of Agrobacterium culture into plant tissues, pollen-tube pathway, floral dip and floral spray are the main methods of in planta transformation. Each of these methods has its own advantages and disadvantages. Simplicity and reliability are the primary reasons for the popularity of the in planta methods. These methods are much faster than regular Agrobacterium-mediated gene transformation based on tissue culture and success can be achieved by non-experts. In the present review, we highlight all methods of in planta transformation comparing them with regular Agrobacterium-mediated transformation methods based on tissue culture. Finally, successful recent transformations using these methods are presented.

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“…Different scientists reported that MS basal medium directly regenerated the plants from inoculated shoots (Murashige and Skoog 1962 ;Shabde and Murashige 1977 ) . The same medium has been used to regenerate other crops such as Gossypium (Gould et al 1991a ) , maize (Gould et al 1991b ) and cereals (Hiei et al 1997 ) . Tissue culture is considered as an essential step for transformation of the majority of plant species, consequently higher priority is needed to establish research procedures that could reduce the somaclonal variation (Birch 1997 ) .…”

Section: Tissue Culturementioning

confidence: 99%

Plant Genetic Engineering: Problems and Applications

Rashid

Husnain

Riazuddin

2012

Crop Production for Agricultural Improvement

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Regeneration of Gossypium hirsutum and G. barbadense from shoot apex tissues for transformation

Cited by 73 publications

References 23 publications

Expression of Mannose-Binding Insecticidal Lectin Gene in Transgenic Cotton (<i>Gossypium</i>) Plant

Expression of Mannose-Binding Insecticidal Lectin Gene in Transgenic Cotton (<i>Gossypium</i>) Plant

Tissue culture-based Agrobacterium-mediated and in planta transformation methods

Plant Genetic Engineering: Problems and Applications

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