Regeneration of healthy plants from Cathranthus roseus infected with mycoplasma-like organisms through callus culture

Möllers, Christian; Sarkar, Sukanya

doi:10.1016/0168-9452(89)90047-2

Cited by 22 publications

(12 citation statements)

References 13 publications

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“…Roots were healthy and strong in appearance (Fig.1A). This result correlates with a previous study that found internodal tissues to be best suited for the regeneration of Catharanthus (Mollers andSarkar, 1989 andSwanberg andDai, 2008 ).…”

Section: Shoot Formationsupporting

confidence: 81%

In Vitro Regeneration and Somaclonal Variation of Catharanthus roseus Don. Using Leaf and Internodal Explants

2013

Alexandria Science Exchange Journal: An International Quarterly

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Section: Shoot Formationsupporting

confidence: 81%

In Vitro Regeneration and Somaclonal Variation of Catharanthus roseus Don. Using Leaf and Internodal Explants

2013

Alexandria Science Exchange Journal: An International Quarterly

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“…Mö llers and Sarkar (1989) infected the internodal stem sections from C. roseus plants with three different MLOs and cultured them on the MS solid nutrient medium. Callus tissue was formed on the infected explants, which then differentiated into plants after transfer onto the MS medium supplemented with 1-naphthyl acetic acid (NAA) 1.0 mg/l, 6-benzyl aminopurine (BAP) 0.25 mg/l and gentamycin 10.0 mg/l.…”

Section: Plant Regeneration Of Catharanthus Roseus (L) G Don In Vitromentioning

confidence: 99%

Catharanthus roseus: micropropagation and in vitro techniques

Pietrosiuk

Furmanowa

Łata³

2007

Phytochem Rev

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Different methods of in vitro culture of Catharanthus roseus provide new sources of plant material for the production of secondary metabolites such as indole alkaloids. Callus, cell suspension, plantlets, and transgenic roots cultured in the bioreactor are used in those experiments.The most promising outcomes include the production of the following indole alkaloids: ajmalicine in unorganised tissue, catharanthine in the leaf and cell culture in the shake flask and airlift bioreactor, and vinblastine in shoots and transformed roots. What is very important, enzymatic coupling of monomeric indole alkaloids, vindoline and catharanthine, is possible to form vinblastine in cell cultures. The method of catharanthine and ajmalicine production in the suspension culture in bioreactors has been successful. In this method, elicitation may be used acting on different metabolic pathways. Also of interest is the method of obtaining arbutin from the callus culture of C. roseus conducted with hydroquinone. The transformed root culture seems to be the most promising for alkaloid production. The genetically transformed roots, obtained by the infection with Agrobacterium rhizogenes, produce higher levels of secondary metabolites than intact plants. Also, whole plants can be regenerated from hairy roots. The content of indole alkaloids in the transformed roots was similar or even higher when compared to the amounts measured in studies of natural roots. The predominant alkaloids in transformed roots are ajmalicine, serpentine, vindoline and catharanthine, found in higher amounts than in untransformed roots. Transformed hairy roots have been also used for encapsulation in calcium alginate to form artificial seeds.

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“…Tip culture has been shown to decrease PP levels sharply (Sears & Klomparens, 1989), since there are few PP propagules in the growing point. Similarly few PPs survived in plantlets regenerated from callus (Moellers & Sarkar, 1989). However, neither of these measures can eliminate PPs from infected plants.…”

Section: Discussionmentioning

confidence: 99%

“…However, tissue culture has been successfully applied for the elimination of PPs. Through tip culture (Sears & Klomparens, 1989) or callus culture (Moellers & Sarkar, 1989), symptomless plants have been obtained and such plants proved phytoplasma free.…”

Section: Introductionmentioning

confidence: 99%

**Elimination of phytoplasma by stem culture from mulberry plants (Morus alba) with dwarf disease**

Dai

Liu³

1997

Plant Pathology

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Stem culture in vitro was attempted for the elimination of mulberry dwarf phytoplasma. Stem segments, from mulberry plants (Morus alba) seriously infected with the phytoplasma, were cultured on Murashige and Skoog (MS) solid nutrient medium without phytohormones for 2-3 months. The tissue cultures, and regenerated plants grown under greenhouse conditions for 3 years, were tested by polymerase chain reaction (PCR) and 4,6-diamidino-2-phenylindole (DAPI) staining for the presence of the phytoplasma, and also observed for mulberry dwarf symptoms. From 70% to 90% of regenerated plants were apparently freed from the phytoplasma since they remained PCR-negative, DAPI-negative and symptom-free for as long as tested (3 years).

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Regeneration of healthy plants from Cathranthus roseus infected with mycoplasma-like organisms through callus culture

Cited by 22 publications

References 13 publications

In Vitro Regeneration and Somaclonal Variation of Catharanthus roseus Don. Using Leaf and Internodal Explants

In Vitro Regeneration and Somaclonal Variation of Catharanthus roseus Don. Using Leaf and Internodal Explants

Catharanthus roseus: micropropagation and in vitro techniques

**Elimination of phytoplasma by stem culture from mulberry plants (Morus alba) with dwarf disease**

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