Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells

Cao, Jun; Duan, Xin; McEIroy, David; Wü, Ray

doi:10.1007/bf00233098

Cited by 217 publications

(113 citation statements)

References 12 publications

Supporting

Mentioning

111

Contrasting

Order By: Relevance

“…Fine suspension culture cells were used as the transformation material and bombarded with tungsten particles coated with the pBY520 plasmid, essentially as described by Cao et al (1992). Resistant calli were selected in selection medium, containing 6 mg L-' ammonium glufosinate (Crescent Chemical Co., Hauppauge, NY) as the selective agent, for 5 to 7 weeks.…”

Section: Production Of Transgenic Rice Plantsmentioning

confidence: 99%

Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice

et al. 1996

View full text Add to dashboard Cite

A late embryogenesis abundant (LEA) protein gene, HVA7, from barley (Hordeum vulgare L.) was introduced into rice suspension cells using the Biolistic-mediated transformation method, and a large number of independent transgenic rice (Oryza sativa L.) plants were generated. Expression of the barley HVA 7 gene regulated by the rice actin 1 gene promoter led to high-level, constitutive accumulation of the HVAl protein in both leaves and roots of transgenic rice plants. Second-generation transgenic rice plants showed significantly increased tolerance to water deficit and salinity. Transgenic rice plants maintained higher growth rates than nontransformed control plants under stress conditions. The increased tolerance was also reflected by delayed development of damage symptoms caused by stress and by improved recovery upon the removal of stress conditions. We also found that the extent of increased stress tolerante correlated with the leve1 of the HVAl protein accumulated in the transgenic rice plants. Using a transgenic approach, this study provides direct evidence supporting the hypothesis that LEA proteins play an important role in the protection of plants under wateror salt-stress conditions. Thus, LEA genes hold considerable potentia1 for use as molecular tools for genetic crop improvement toward stress tolerance.

show abstract

Section: Production Of Transgenic Rice Plantsmentioning

confidence: 99%

Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice

et al. 1996

View full text Add to dashboard Cite

show abstract

“…The surviving plants further subjected to Basta spray revealed the presence of bar gene in transformed plants. Many researchers have reported the tolerance of crop plants to phosphinothricin after the successful transformation of the bar gene in crops like maize (Gordon-Kamm et al, 1990), rice (Cao et al, 1992), wheat (Weeks et al, 1993), sugarcane (Manickavasagam et al, 2004), and cowpea (Ilori et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Phosphinothricin N-acetyltransferase encodes phosphinothricin, the active ingredient of herbicides such as Basta®, by acetylation. The bar gene has been widely used as an effective selectable marker in many crop species like maize (Gordon-Kamm et al, 1990), rice (Cao et al, 1992), wheat (Weeks et al, 1993), sugarcane (Manickavasagam et al, 2004), and cowpea (Ilori et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Production of herbicide-resistant cowpea (Vigna unguiculata L.) transformed with the bar gene

Aasım¹,

Khawar²,

Özcan³

2013

Turk J Biol

View full text Add to dashboard Cite

Plant genetic engineering has opened new avenues to modify crops and has provided a powerful tool for crop improvement.\ud The present study reports the development of regeneration and genetic transformation protocol for the Turkish cowpea cultivar Akkiz.\ud The immature cotyledons were cultured on Murashige and Skoog (MS) medium containing 0.25–0.75 mg L–1 6-benzylaminopurine with\ud or without 0.25 mg L–1 α-naphthalene acetic acid. Shoot regeneration varied 44.4%–83.3% with 2.1–5.0 shoots per explant. Regenerated\ud shoots were rooted in MS medium containing 0.50 mg L–1 indole-3-butyric acid and acclimatized in the greenhouse, where they flowered\ud and set seeds. Immature cotyledons were transformed with Agrobacterium tumefaciens strain LBA4404 harboring the recombinant\ud binary vector pRGG containing an herbicide tolerance gene (bar) along with a uida (GUS) gene under 35S promoter. Phosphinothricin\ud was used as a selectable marker at a concentration of 2.5 mg L–1. Putative transformants were screened by the histochemical GUS assay.\ud Furthermore, molecular analysis revealed the presence of the introduced gene in the genome of cowpea cultivar Akkiz. The selected\ud transgenic plants showed a resistance to Basta® nonselective herbicide at up to 10 mL L–1 of water. Putative transgenic plants retained\ud their pigmentation and continued to grow in the greenhouse

show abstract

“…Plasmid pDM302 contains the promoter, first exon and first intron of the rice Actin1 gene, the bar coding sequence and nos terminator sequence (Cao et al 1992). The bar gene confers resistance to the herbicide phosphinothricin.…”

Section: Plasmid Vectors and Genetic Transformation Of Wheatmentioning

confidence: 99%

Increased tolerance to wheat powdery mildew by heterologous constitutive expression of the Solanum chacoense Snakin-1 gene

Faccio¹,

Rovere²,

Hopp³

et al. 2011

CZECH J GENET PLANT

View full text Add to dashboard Cite

Great efforts are currently being devoted to studying the use of transgenes to confer resistance to phytopathogenic fungi. Snakin-1 is a broad-spectrum antimicrobial peptide isolated from Solanum that is active in vitro against bacteria and fungi. Recently, it was reported that overexpression of the snakin-1 (SN1) gene in transgenic potato plants enhanced resistance to Rhizoctonia solani and Erwinia carotovora. In this work wheat transgenic plants that constitutively expressed the S. chacoense SN1 gene were challenged with Blumeria graminis f.sp. tritici. Enhanced resistance to the pathogen was observed in two transgenic lines in which the development of the disease was delayed and reduced compared with the wild type variety ProINTA Federal. An association between high resistance to the pathogen and a high level of snakin-1 transcripts in the plant was observed. This is the first report on SN1 gene expression in Gramineae and its effects on wheat powdery mildew development.

show abstract

Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells

Cited by 217 publications

References 12 publications

Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice

Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice

Production of herbicide-resistant cowpea (Vigna unguiculata L.) transformed with the bar gene

Increased tolerance to wheat powdery mildew by heterologous constitutive expression of the Solanum chacoense Snakin-1 gene

Contact Info

Product

Resources

About