Regeneration of Intervertebral Disc Using Poly(lactic-co-glycolic acid) Scaffolds Included Demineralized Bone Particle In Vivo

Abstract

Demineralized bone particle (DBP) is a biomaterial used widely in the field of tissue engineering. In this study, in order to study the effect of DBP/poly(lactic-co-glycolic acid) (PLGA) scaffold on disc regeneration in vivo environment, we prepared the porous DBP/PLGA hybrid scaffold. Disc defect was induced by removing the nucleus pulposus tissue after incision the annulus fibrosus tissue in half and scaffolds were transplanted. After 1, 2 and 3 months later, the extracted discs were confirmed by collagen synthesis and glycosaminoglycan (sGAG). We conducted histology (H&E, Safranin-O, Alcian blue, Type I Collagen, Type II Collagen). From the results, it was confirmed that collagen and sGAG content were high in DBP/PLGA scaffold, and the regeneration of intervertebral disc was possible.

Preparation, the Chain-extending Reaction Kinetics and Thermal Degradation of Poly(D-lactide) by Reactive Extrusion Process

“…A number of factors contribute to the success of polylactide (PLA) in these applications like in textiles, medical field, and also in the packaging industry 1,2 including its physical properties, favorable degradation characteristics, and benign degradation products. 3 However until now, PLA still has some disadvantages. For instance, slow crystallization rate, low crystalline degree and poor heat resistance of PLA have restricted its practical applications.…”

Performance and Structure Characterization of Poly(lactic acid)/Zinc Oxide Pillared Organic Saponite Nanocomposites by Solution Intercalation

“…1 PLA has low toxicity and good barrier properties, which served this polymer as a potential candidate into wide range of industrial applications, such as food packaging, textile industries and many other engineering fields. 2,3 On the other hand, the main disadvantage of PLA is its low crystallinity, which makes it highly brittle, consequently lowering its mechanical strength. [4][5][6] In order to overcome this problem, researchers synthesized polymer/clay nanocomposites, which can be a potential candidate because of their excellent mechanical properties, thermal stability, gas barrier, and optical properties with low clay content.…”