Regeneration of irradiated salivary glands with stem cell marker expressing cells

Nanduri, Lalitha S.Y.; Maimets, Martti; Pringle, Sarah; Zwaag, Marianne van der; Os, Ronald P. van; Coppes, Robert P.

doi:10.1016/j.radonc.2011.05.085

Cited by 156 publications

(162 citation statements)

References 25 publications

Supporting

Mentioning

150

Contrasting

Unclassified

Order By: Relevance

“…Clinically, obstructive functional disorder of the salivary gland is a pathology that develops over a long period of time, due to increases in salivary calculi or other substances. To date, reports on submandibular gland main excretory duct ligation models have mostly been short ligation periods of around 1 week [19][20][21][22][23] , and the longest have been approximately 1 month 8,11) . Thus, there is a need for studies investigating the tendency toward atrophy and residual regeneration function in salivary glands in conditions closer to actual clinical pathology.…”

Section: Discussionmentioning

confidence: 99%

“…Studies of residual salivary gland function and regeneration function in atrophied salivary glands expression of the acinar cell markers -amylase [24][25][26] and AQP5 23,[26][27][28] , and stem-cell markers such as c-kit [19][20][21][22][23] are investigated. However, all of these results are from short-term ligation.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, duct-like structures were examined from the perspective of evaluating the regenerative capacity of atrophied tissue, using the stem cell markers c-kit and Sca-1. Cells that are positive for c-kit and Sca-1 have been reported in salivary gland ducts and their surroundings [19][20][21][22] . In the present study, localization of cells positive for stem cell markers was observed in duct-like structures and their surroundings.…”

Section: Discussionmentioning

confidence: 99%

“…Changes in salivary gland tissue that have been found as a result of duct ligation include atrophy and disappearance of acinar cells above the ligation, an increase in duct-like structures, and an increase in fibrous connective tissue [8][9][10][11][12][13][14][15][16][17][18] . There are also reports noting residual salivary gland function, restorative function, and regenerative ability in atrophied salivary glands [19][20][21][22][23] . However, most of these studies had a short ligation period, and there have been no studies observing progress over the long term.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

Murayama

Kawakami

Tanaka

2017

J. Hard Tissue Biology.

View full text Add to dashboard Cite

To examine the pathology of salivary glands that have undergone atrophy or hypofunction due to old age or disease, the duct ligation model is used. This model has been used in studies on the course of glandular parenchyma atrophy and the potential for repair and regeneration. However, the period of ligation was short in most of these studies, and none have examined long-term progress. Therefore, we investigated long-term ligation of the submandibular gland in mice and examined the chronic changes in atrophied salivary glands. The ligation periods were 1, 2 and 3 months, and atrophied salivary glands were resected after each period. Resected glands were examined by histology, immunohistochemistry, RT-PCR, and transmission electron microscopy. Histologically, disappearance of acinar cells and increases in duct-like structures were observed over time in atrophied salivary glands, resulting from long-term submandibular gland main excretory duct ligation. Acinar cell markers (-amylase, aquaporin 5) showed marked weakness in expression after ligation, but expression was still observed after 3 months of ligation. Stem cell markers (c-kit, Sca-1) showed greater expression at 1 month of ligation, compared with controls, but expression subsequently decreased with time. Expression of the precursor cell marker cytokeratin 5 was retained throughout long-term ligation. Atrophied salivary gland tissue resulting from long-term ligation showed increases in specific duct-like structures over time, and the expression of stem cell markers and progenitor cell markers in the area of these structures suggests that the repair capability remained intact.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

Murayama

Kawakami

Tanaka

2017

J. Hard Tissue Biology.

View full text Add to dashboard Cite

show abstract

“…[55] was able to show immune-histochemical staining of several stem cell markers in the ductal compartment of mouse submandibular glands including c-Kit, CD133, CD24, CD29, and CD49f. Interestingly, culturing gland cells as salispheres, enriches a c-Kit+ population after several days [56]. As reported by Lombaert et.…”

Section: Stem Cellsmentioning

confidence: 60%

A systems biology approach identifies a gene regulatory network in parotid acinar cell differentiation.

Metzler¹

View full text Add to dashboard Cite

ACKNOWLEDGEMENTSThis study was undertaken with the help and support of many individuals. I would first like to thank my advisor Dr. Doug Darling who was always available for questions and advise, and whose continued support of myself and this project through difficult periods was essential and much appreciated. His guidance has helped me to develop as a scientist.I also worked closely with Dr. Srirangapatnam Venkatesh when I first started. His friendly and helpful attitude could always be counted on, and was wonderful to have during my initial training. He is greatly missed by all who knew him.

show abstract

ΔNp63 is upregulated during salivary gland regeneration following duct ligation and irradiation in mice

et al. 2020

View full text Add to dashboard Cite

The transcription factor p63, a component of the p53 family, has important functions in development, homeostasis, and regeneration of epithelial tissues. However, the role of p63 in the regeneration of exocrine glands, including the salivary glands (SGs), has not been fully investigated. We investigated p63 expression in SG regeneration induced by duct ligation and irradiation. The expression of DNp63, a p63 isoform, increased and was colocalized with keratin 5 positive cells were myoepithelial cells. Furthermore, DNp63 expression was regulated by FGF7 stimulation via p38 MAPK phosphorylation and affected SG morphogenesis. These results suggest that DNp63 is essential for SG regeneration and may be a new target for regenerative treatment.

show abstract

Regeneration of irradiated salivary glands with stem cell marker expressing cells

Abstract: Different stem cell-associated markers are expressed in mouse salivary gland cells, which upon transplantation are able to regenerate the irradiation damaged salivary gland.

Cited by 156 publications

References 25 publications

Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

A systems biology approach identifies a gene regulatory network in parotid acinar cell differentiation.

ΔNp63 is upregulated during salivary gland regeneration following duct ligation and irradiation in mice

Contact Info

Product

Resources

About