Regeneration of Lonicera tatarica plants via adventitious organogenesis from cultured stem explants

Abstract

We describe an efficient process for the regeneration of Lonicera tatarica plants from cultured stem sections. Induction of multiple shoots was achieved directly from cultured stem cuttings. The highest regeneration rate was achieved an Gamborg's B5 medium supplemented with 4% sucrose and 0.8% Difco bacto-agar in the absence of hormones. Differentiated shoots were elongated for 5-7 days an induction medium supplemented with 0.5 mg/l GA3. Shoot induction and elongation experiments were carried out using original stem explants from either 2-, 6-, or 18-month-old donor plants. The age of the donor plant had no noticeable effect on either process. However, rooting of elongated shoots occurred only with shoots derived from 2-month-old donor plants. Rooting efficiency and proliferation were highest on half-strength WPM medium supplemented with 2 µM indole-3-butyric acid and 0.6% Keylis agar. The plants regenerated from stem explants were morphologically normal, and levels of loganin and secologanin were comparable to those detected in plants grown from seed and maintained through vegetative propagation

An Efficient Procedure for Regeneration from Leaf-derived Calluses of Lonicera macranthoides ‘Jincuilei’, an Important Medicinal Plant

“…In vitro culture has been used to manipulate several Lonicera species, and in some instances, whole plants have been regenerated. Shoot proliferation was achieved using single nodes of L. periclymenum L. (Boonnour et al, 1988), L. caerulea L. (Karhu, 1997), and L. tatarica (Palacios et al, 2002). Indirect shoot organogenesis was established using leaf explants of L. japonica (Georges et al, 1993) and L. nitida E. H. Wilson (Cambecedes et al, 1991) as well as leaf protoplasts of L. nitida (Ochatt, 1991).…”

“…Dried flowers and buds of honeysuckles are called ''Jinyinhua'' in Chinese or flos lonicerae, which is a famous herb of traditional Chinese medicine. Extracts of flos lonicerae have a wide range of benefits to human health, including anti-inflammation, bacteriostasis, antivirus, antioxidant, and hematischesis (Lee et al, 1998;Palacios et al, 2002;Peng et al, 2005). Flos lonicerae contains a series of water-soluble phenolic compounds with 3-O-ceffeoyl-D-quinic acid or chlorogenic acid (CGA) being a major ingredient.…”

“…CGA has been studied in L. confuse DC., L. dasystyla Rehder, L. hypoglauca Miq. (Guo and Wan, 2005;Li et al, 2003), L. japonica (Lee et al, 1998), and L. tatarica L. (Palacios et al, 2002).…”

‘Longhua’ Lonicera

“…Dried flowers and buds of honeysuckles are known as ''Jinyinhua'' in Chinese or flos lonicerae, which is one of the most popular herbs of traditional Chinese medicine (Shang et al, 2011). Flos lonicerae contains a series of compounds including essential oils, organic acids, flavones, saponins, and iridoids (Lee et al, 1998;Palacios et al, 2002). Because of the variation among species and even cultivars in the chemical compounds, the Chinese Pharmacopoeia (Committee for the Pharmacopoeia of PR China, 2010) has used the content of 5-O-caffeoylquinic acid or chlorogenic acid (CGA) as the indicator compound for the evaluation of the quality of flos lonicerae (Shang et al, 2011).…”

Screening existing black pepper (Piper nigrum L. Family Piperaceae) cultivars for superior spice value (high essential oil, oleoresin and piperine content) and their in vitro propagation

“…Meanwhile regeneration of Lonicera tatarica plants via adventitious organogenesis from stem explants has been carried out. (Palacios et al, 2001). This method is used to produce a large number of desirable plants.…”